| Gene Symbol | L3MBTL1 |
|---|---|
| Entrez Gene | 26013 |
| Alt Symbol | H-L(3)MBT, L3MBTL, ZC2HC3, dJ138B7.3 |
| Species | Human |
| Gene Type | protein-coding |
| Description | l(3)mbt-like 1 (Drosophila) |
| Other Description | l(3)mbt protein homolog|lethal (3) malignant brain tumor l(3)|lethal(3)malignant brain tumor-like protein 1 |
| Swissprots | Q9UJB9 Q9H1E6 Q9H1G5 E1P5W7 Q5H8Y8 Q9Y4C9 B4DRC9 Q8IUV7 Q9UG06 Q5H8Y9 Q9Y468 |
| Accessions | EAW75957 EAW75958 EAW75959 EAW75960 EAW75961 EAW75962 Q9Y468 AB014581 BAA31656 AK123176 BAC85548 AK299199 BAG61241 AK308334 AK310345 AL110279 CAB53714 BC034398 BC039820 AAH39820 BG956397 BI040370 BU741824 DA204976 DR003568 EU446490 ABZ92019 R42592 NM_015478 NP_056293 NM_032107 NP_115479 |
| Function | Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post- translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis. {ECO:0000269|PubMed:17540172, ECO:0000269|PubMed:18408754, ECO:0000269|PubMed:20870719, ECO:0000269|PubMed:20870 |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:10445843}. Note=Excluded from the nucleolus. Does not colocalizes with the PcG protein BMI1, suggesting that these two proteins do not belong to the same complex. |
| Tissue Specificity | Widely expressed. Expression is reduced in colorectal cancer cell line SW480 and promyelocytic leukemia cell line HL-60. {ECO:0000269|PubMed:10445843}. |
L3MBTL CRISPR/Cas9 KO Plasmid (h) - sc-410575 from Santa Cruz Biotechnology
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L3MBTL HDR Plasmid (h) - sc-410575-HDR from Santa Cruz Biotechnology
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L3MBTL Double Nickase Plasmid (h) - sc-410575-NIC from Santa Cruz Biotechnology
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L3MBTL Double Nickase Plasmid (h2) - sc-410575-NIC-2 from Santa Cruz Biotechnology
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L3MBTL CRISPR Activation Plasmid (h) - sc-410575-ACT from Santa Cruz Biotechnology
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L3MBTL CRISPR Activation Plasmid (h2) - sc-410575-ACT-2 from Santa Cruz Biotechnology
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L3MBTL Lentiviral Activation Particles (h) - sc-410575-LAC from Santa Cruz Biotechnology
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L3MBTL Lentiviral Activation Particles (h2) - sc-410575-LAC-2 from Santa Cruz Biotechnology
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L3MBTL siRNA (h) - sc-60915 from Santa Cruz Biotechnology
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L3MBTL shRNA Plasmid (h) - sc-60915-SH from Santa Cruz Biotechnology
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L3MBTL shRNA (h) Lentiviral Particles - sc-60915-V from Santa Cruz Biotechnology
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L3MBTL1 RNAi - H00026013-R01 from Novus Biologicals
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MISSION® esiRNA - EHU144471 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN2408 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND2408 from Sigma-Aldrich
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L3MBTL1 siRNA (Human) - CRH8653 from Cohesion Biosciences
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L3MBTL1 RNAi - H00026013-R02 from Novus Biologicals
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shRNA set against Human L3MBTL1 (NM_015478.6) - SHH328039 from Creative biogene
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Human L3MBTL1 siRNA - orb269155 from Biorbyt
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L3MBTL1 shRNA Plasmid (Human) - CHH8653 from Cohesion Biosciences
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L3MBTL1 miRNA 3'UTR clone - MiUTR3H-10246 from Creative biogene
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L3MBTL1 miRNA 3'UTR clone - MiUTR3H-10247 from Creative biogene
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