PAAF1

Gene Symbol PAAF1
Entrez Gene 80227
Alt Symbol PAAF, Rpn14, WDR71
Species Human
Gene Type protein-coding
Description proteasomal ATPase-associated factor 1
Other Description WD repeat domain 71|WD repeat-containing protein 71
Swissprots Q7Z500 Q53HS9 B7ZAS9 Q8TBU6 Q4G165 B4DPB0 A6NDR5 Q9BRP4 Q9HAB6
Accessions EAW74917 EAW74918 EAW74919 Q9BRP4 AF087895 AAP97194 AK021910 BAB13933 AK222501 BAD96221 AK225520 AK298258 BAG60522 AK309133 AK316394 BAH14765 BC006142 AAH06142 BC021541 AAH21541 BC028628 AAH28628 BC028677 BC045774 CD243380 DC354686 HQ448172 ADQ32653 XM_005274308 XP_005274365 XM_005274309 XP_005274366 XM_005274310 XP_005274367 XM_011545272 XP_011543574 XM_011545273 XP_011543575 XR_950069 NM_001267803 NP_001254732 NM_001267804 NP_001254733 NM_001267805 NP_001254734 NM_001267806 NP_001254735 NM_025155 NP_079431
Function Inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency. Protects SUPT6H from proteasomal degradation. {ECO:0000269|PubMed:15831487, ECO:0000269|PubMed:17289585, ECO:0000269|PubMed:22316138}.
Tissue Specificity Ubiquitously expressed, with highest levels in kidney, brain and testis. {ECO:0000269|PubMed:15831487}.

PAAF1 (G-15) - sc-240765 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PAAF1 (N-16) - sc-240764 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PAAF1 antibody - ab103566 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PAAF1 antibody (ab103566) at 1/100 dilution + A549 cell lysate at 35 µg

Anti-PAAF1 antibody - ab118625 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAAF1 antibody (ab118625) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysate

Anti-PAAF1 Antibody (aa109-138, AP) - LS-C216585 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAAF1 Antibody (aa109-138, APC) - LS-C216588 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAAF1 Antibody (aa109-138, Biotin) - LS-C216589 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAAF1 Antibody (aa109-138, FITC) - LS-C216590 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAAF1 Antibody (aa109-138, PE) - LS-C216592 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAAF1 Antibody (aa109-138, Azide-free, HRP) - LS-C216591 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAAF1 Antibody (aa115-145) - LS-C323745 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAAF1 Antibody (aa57-106) - LS-C169831 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PAAF1 Antibody (clone 2G6) - LS-C338418 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G6
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAAF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAAF1.

Anti-PAAF1 Antibody (aa109-138) - LS-C163330 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PAAF1 Antibody IHC of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-PAAF1 Antibody (clone 1F11) - LS-C338416 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F11
Anti-PAAF1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PAAF1.

PAAF1 Antibody - NBP1-82310 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PAAF1 Antibody [NBP1-82310] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

Anti-PAAF1 antibody produced in rabbit - HPA039952 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PAAF1: <B>Cat. No. HPA039952</B>: Immunohistochemical staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.

Anti-PAAF1 (111-125) antibody produced in rabbit - SAB1102201 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human PAAF1 was separated on SDS-PAGE, blotted with Anti-PAAF1 (111-125) (<B>Cat. No. SAB1102201</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250

PAAF1 Antibody - N-terminal region (ARP66899_P050) - ARP66899_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PAAF1 Antibody - N-terminal region (OAAB02477) - OAAB02477 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

PAAF1-Antibody-N-term - AP5062a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of PAAF1 Antibody (N-term) (Cat. #AP5062a) in A549 cell line lysates (35ug/lane).PAAF1 (arrow) was detected using the purified Pab.

PAAF1 antibody - orb77802 from Biorbyt

Prices $425.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Western blot analysis of Hela and Jurkat cell lysate using PAAF1 antibody

PAAF1 Antibody - abx032396 from Abbexa

Host Rabbit
Clonality Polyclonal

PAAF1 Antibody - abx038032 from Abbexa

Host Rabbit
Clonality Polyclonal

PAAF1 antibody - 17650-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human kidney tissue slide using 17650-1-AP( PAAF1 Antibody) at dilution of 1:50 (under 10x lens)
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