UBE3C

Gene Symbol UBE3C
Entrez Gene 9690
Alt Symbol HECTH2
Species Human
Gene Type protein-coding
Description ubiquitin protein ligase E3C
Other Description ubiquitin-protein isopeptide ligase (E3)|ubiquitin-protein ligase E3C
Swissprots Q9UDU3 Q15386 A6NCP3 Q8TC15 Q96CR4 A4D235
Accessions AAD51453 EAL23922 EAX04567 EAX04568 EAX04569 Q15386 AA596042 AK127280 AK295138 BAG58161 BC014029 AAH14029 BC026241 AAH26241 CD366848 D13635 BAA02799 DB085748 DB454915 XM_005249564 XP_005249621 XM_011516714 XP_011515016 XR_927552 NM_014671 NP_055486
Function E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo. {ECO:0000269|PubMed:11278995, ECO:0000269|PubMed:12692129, ECO:0000269|PubMed:16341092, ECO:0000269|PubMed:16601690}.
Subcellular Location Nucleus {ECO:0000305}.
Tissue Specificity Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas. {ECO:0000269|PubMed:11278995, ECO:0000269|PubMed:12692129, ECO:0000269|PubMed:9575161}.
Top Pathways Ubiquitin mediated proteolysis

UBE3C (S-14) - sc-165851 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-UBE3C antibody - ab101512 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
IHC image of UBE3C staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101512, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-UBE3C antibody - ab180113 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-UBE3C antibody (ab180113) at 1/1000 dilution + HeLa cell line lysate at 35 µgdeveloped using the ECL technique

Anti-UBE3C antibody - ab68225 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-UBE3C antibody (ab68225) at 1/500 dilutionLane 1 : UBE3C transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-UBE3C antibody - ab172310 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-UBE3C antibody (ab172310) at 1 µg/mlLane 1 : Lysate from 293T cells transfected with UBE3CLane 2 : Lysate from untransfected 293T cellsLysates/proteins at 15 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-UBE3C Antibody (aa725-775) - LS-C290767 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-UBE3C Antibody (aa1-50) - LS-C205550 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
UBE3C antibody Western Blot of THP-1. Antibody dilution: 1 ug/ml.

Anti-UBE3C Antibody - LS-C335670 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using UBE3C antibody.

Anti-UBE3C Antibody (aa350-400) - LS-C290765 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human UBE3C by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-UBE3C antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-UBE3C Antibody (aa549-578) - LS-C204005 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
UBE3C Antibody western blot of HeLa cell line lysates (35 ug/lane). The UBE3C antibody detected the UBE3C protein (arrow).

UBE3C Antibody - NBP2-20790 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: UBE3C Antibody [NBP2-20790] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:500.

UBE3C Antibody - NBP1-81558 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: UBE3C Antibody [NBP1-81558] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

UBE3C Antibody - H00009690-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: UBE3C Antibody [H00009690-B01] - analysis of UBE3C expression in transfected 293T cell line by UBE3C MaxPab polyclonal antibody (B01). Lane 1: UBE3C transfected lysate (45.80 KDa). Lane 2: Non-transfected lysate.

UBE3C Antibody - H00009690-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: UBE3C Antibody [H00009690-B01P] - Analysis of UBE3C expression in transfected 293T cell line by UBE3C polyclonal antibody. Lane 1: UBE3C transfected lysate(44.44 KDa). Lane 2: Non-transfected lysate.

UBE3C Antibody - H00009690-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

UBE3C Antibody - H00009690-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-UBE3C antibody produced in rabbit - SAB2702106 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-UBE3C: <B>Cat. No. SAB2702106</B>: Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE3C antibody at a 1:500 dilution.

Anti-UBE3C antibody produced in mouse - SAB1406868 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-UBE3C: <B>Cat. No. SAB1406868</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~44.4 kDa using UBE3C transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-UBE3C antibody produced in rabbit - HPA039915 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-UBE3C: <B>Cat. No. HPA039915</B>: Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Anti-UBE3C antibody produced in rabbit - SAB1411691 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-UBE3C: <B>Cat. No. SAB1411691</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~45.8 kDa using UBE3C transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

UBE3C Antibody - N-terminal region (ARP43163_P050) - ARP43163_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

UBE3C-Antibody-Center - AP20457c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of lysates from Hela cell line, mouse brain tissue lysate, HepG2 cell line (from left to right), using UBE3CAntibody(Center)(Cat. #AP20457c). AP20457c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

UBE3C Antibody - middle region (OAAB17981) - OAAB17981 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

UBE3C Polyclonal Antibody - A6442 from ABclonal

Prices $130.00, $220.00, $380.00
Sizes 50 µl, 100 µl, 200 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using UBE3C antibody.

UBE3C Antibody - abx034815 from Abbexa

Host Rabbit
Clonality Polyclonal
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