Gene Symbol | UBE3C |
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Entrez Gene | 9690 |
Alt Symbol | HECTH2 |
Species | Human |
Gene Type | protein-coding |
Description | ubiquitin protein ligase E3C |
Other Description | ubiquitin-protein isopeptide ligase (E3)|ubiquitin-protein ligase E3C |
Swissprots | Q9UDU3 Q15386 A6NCP3 Q8TC15 Q96CR4 A4D235 |
Accessions | AAD51453 EAL23922 EAX04567 EAX04568 EAX04569 Q15386 AA596042 AK127280 AK295138 BAG58161 BC014029 AAH14029 BC026241 AAH26241 CD366848 D13635 BAA02799 DB085748 DB454915 XM_005249564 XP_005249621 XM_011516714 XP_011515016 XR_927552 NM_014671 NP_055486 |
Function | E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo. {ECO:0000269|PubMed:11278995, ECO:0000269|PubMed:12692129, ECO:0000269|PubMed:16341092, ECO:0000269|PubMed:16601690}. |
Subcellular Location | Nucleus {ECO:0000305}. |
Tissue Specificity | Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas. {ECO:0000269|PubMed:11278995, ECO:0000269|PubMed:12692129, ECO:0000269|PubMed:9575161}. |
Top Pathways | Ubiquitin mediated proteolysis |
UBE3C CRISPR/Cas9 KO Plasmid (h) - sc-410554 from Santa Cruz Biotechnology
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UBE3C HDR Plasmid (h) - sc-410554-HDR from Santa Cruz Biotechnology
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UBE3C Double Nickase Plasmid (h) - sc-410554-NIC from Santa Cruz Biotechnology
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UBE3C Double Nickase Plasmid (h2) - sc-410554-NIC-2 from Santa Cruz Biotechnology
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UBE3C CRISPR Activation Plasmid (h) - sc-410554-ACT from Santa Cruz Biotechnology
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UBE3C CRISPR Activation Plasmid (h2) - sc-410554-ACT-2 from Santa Cruz Biotechnology
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UBE3C Lentiviral Activation Particles (h) - sc-410554-LAC from Santa Cruz Biotechnology
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UBE3C Lentiviral Activation Particles (h2) - sc-410554-LAC-2 from Santa Cruz Biotechnology
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UBE3C siRNA (h) - sc-89538 from Santa Cruz Biotechnology
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UBE3C shRNA Plasmid (h) - sc-89538-SH from Santa Cruz Biotechnology
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UBE3C shRNA (h) Lentiviral Particles - sc-89538-V from Santa Cruz Biotechnology
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UBE3C RNAi - H00009690-R01 from Novus Biologicals
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MISSION® esiRNA - EHU021021 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22394 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22394 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR12702 from Sigma-Aldrich
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UBE3C siRNA (Human) - CRH6462 from Cohesion Biosciences
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UBE3C - MBS8237023 from MyBioSource
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shRNA set against Human UBE3C (NM_014671.2) - SHH439276 from Creative biogene
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Human UBE3C siRNA - orb266964 from Biorbyt
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UBE3C shRNA Plasmid (Human) - CHH6462 from Cohesion Biosciences
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UBE3C miRNA 3'UTR clone - MiUTR3H-13257 from Creative biogene
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