PRC1

Gene Symbol PRC1
Entrez Gene 9055
Alt Symbol ASE1
Species Human
Gene Type protein-coding
Description protein regulator of cytokinesis 1
Other Description anaphase spindle elongation 1 homolog|protein regulating cytokinesis 1
Swissprots B4DLR1 H9KV59 O43663 Q9BSB6 A6NC44
Accessions CAM84944 CAM84945 CAM84946 EAX02134 EAX02135 EAX02136 EAX02137 EAX02138 O43663 AF044588 AAC02688 AK297117 BAG59623 AK304094 BAG65000 AK312600 BC003138 AAH03138 BC005140 AAH05140 BG773441 BG823292 BU634391 BX457401 BX647317 DA655494 DQ892933 ABM83859 DQ896182 ABM87181 DW423775 XM_005254987 XP_005255044 XM_006720759 XP_006720822 XM_006720760 XP_006720823 XM_011522187 XP_011520489 XM_011522188 XP_011520490 XM_011522189 XP_011520491 XM_011522190 XP_011520492 XM_011522191 XP_011520493 XM_011522192 XP_011520494 NM_001267580 NP_001254509 NM_003981 NP_003972 NM_199413 NP_955445 NM_199414
Function Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. {ECO:0000269|PubMed:12082078, ECO:0000269|PubMed:15297875, ECO:0000269|PubMed:15625105, ECO:0000269|PubMed:16431929, ECO:0000269|PubMed:19468300, ECO:0000269|PubMed:20691902, ECO:0000269|PubMed:9885575}.
Subcellular Location Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Note=Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis.

PRC1 Antibody - 3639 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using PRC1 Antibody.

p-PRC1 (C-2) - sc-377544 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PRC1 phosphorylation in untreated (A,D), Ser/Thr induction cocktail (sc-362324) treated (B,E) and Ser/Thr induction cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-PRC1 (C-2): sc-377544 (A,B,C) and PRC1 (6G2): sc-56345 (D,E,F).

PRC1 (H-70) - sc-8356 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PRC1 (H-70): sc-8356. Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.

PRC1 (K-18) - sc-9342 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PRC1 (K-18): sc-9342. Western blot analysis of human recombinant PRC1 fusion protein.

PRC1 (6G2) - sc-56345 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PRC1 phosphorylation in untreated (A,D), Ser/Thr induction cocktail (sc-362324) treated (B,E) and Ser/Thr induction cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-PRC1 (C-2): sc-377544 (A,B,C) and PRC1 (6G2): sc-56345 (D,E,F).

p-PRC1 (Thr 481) - sc-11768 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PRC1 (phospho T481) antibody [EP1514Y] - ab62366 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1514Y
All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilutionLane 1 : HeLa cell lysates (untreated)Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazoleLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP labeled, at 1/2000 dilution

Anti-PRC1 antibody - ab140033 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence of PRC1 in mouse skeletal muscle tissue with ab140033 at 20 ug/mL.

Anti-PRC1 antibody - ab189887 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling PRC1 with ab189887 at 5 µg/ml.

Anti-PRC1 antibody - ab103096 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PRC1 antibody (ab103096) at 1/50 dilution + MDA-MB231 cell lysate at 35 µg

Anti-PRC1 antibody [16F2] - ab119338 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 16F2
Immunofluorescent analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) (ab119338) shows staining in U251 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 (ab119338) at a dilution of 1:20 over night at 4oC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Anti-PRC1 antibody [6G2] - ab39069 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6G2
Anti-PRC1 antibody [6G2] (ab39069) at 1 µg/ml + Hela cell extractsSecondarygoat anti-mouse secondary conjugated to HRPdeveloped using the ECL technique

Anti-PRC1 antibody [EP1513Y] - ab51248 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1513Y
Anti-PRC1 antibody [EP1513Y] (ab51248) at 1/10000 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Anti-PRC1 Antibody (phospho-Thr481) - LS-C291521 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PRC1 Antibody (aa1-150, clone 1D4B) - LS-C189752 from LifeSpan Bioscience

Prices $335.00
Sizes 25 µg
Host Mouse
Clonality Monoclonal
Clone 1D4B

Anti-PRC1 Antibody (clone 6G2) - LS-C41137 from LifeSpan Bioscience

Prices $285.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6G2
Hela cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody reactive against PRC1 (clone 6G2). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence

Anti-PRC1 Antibody (aa432-481) - LS-C199977 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HeLa cells, using PRC1 antibody.

Anti-PRC1 Antibody (phospho-Thr481, clone EP1514Y) - LS-C50129 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1514Y
Immunofluorescent staining of HeLa cells using anti-PRC1 antibody.

Anti-PRC1 Antibody (clone EP1513Y) - LS-C49919 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1513Y
IHC of paraffin-embedded human cervical carcinoma using anti-PRC1 antibody.

Anti-PRC1 Antibody (Internal) IHC-plus™ - LS-B8823 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PRC1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PRC1 Antibody (EP1513Y) - NB110-57434 from Novus Biologicals

Prices $409.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1513Y
Western Blot: PRC1 Antibody (EP1513Y) [NB110-57434] HeLa cells.

PRC1 Antibody - NBP1-88856 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PRC1 Antibody [NBP1-88856] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

PRC1 Antibody - NBP2-19925 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PRC1 Antibody [NBP2-19925] - Sample (30 ug of whole cell lysate) A: JurKat B: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.

PRC1 Antibody (16F2) - NB100-74508 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 16F2
Immunocytochemistry/Immunofluorescence: PRC1 Antibody (16F2) [NB100-74508] - Analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) shows staining in U251 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing PRC1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

PRC1 Antibody (6G2) - NB100-78485 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6G2
Western Blot: PRC1 Antibody (6G2) [NB100-78485] - la cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibody reactive against PRC1 (clone 6G2). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system
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