SF3B1

Gene Symbol SF3B1
Entrez Gene 23451
Alt Symbol Hsh155, MDS, PRP10, PRPF10, SAP155, SF3b155
Species Human
Gene Type protein-coding
Description splicing factor 3b, subunit 1, 155kDa
Other Description SAP 155|pre-mRNA processing 10|pre-mRNA splicing factor SF3b, 155 kDa subunit|spliceosome-associated protein 155|splicing factor 3B subunit 1|splicing factor 3b, subunit 1, 155kD
Swissprots E9PCH3 O75533
Accessions EAW70146 O75533 AF054284 AAC97189 AF070540 AAC28633 AF086296 AK057684 AK290470 BAF83159 AK294851 BAG57955 AK308122 AL137410 CAB70728 BC015530 AAH15530 BC029418 AAH29418 BC039020 BC046140 BC056155 AAH56155 BC064335 BC107883 AAI07884 BE168209 BE168220 CD692669 HY054349 XM_011510867 XP_011509169 XM_011510868 XP_011509170 XR_241300 XR_241302 NM_001005526 NP_001005526 NM_001308824 NP_001295753 NM_012433 NP_036565
Function Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Subcellular Location Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.
Top Pathways Spliceosome

SF3B1 (D7L5T) Rabbit mAb - 14434 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7L5T
Western blot analysis of extracts from various cell lines using SF3B1 (D7L5T) Rabbit mAb.

SAP 155 (G-14) - sc-132446 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SAP 155 (G-14): sc-132446. Western blot analysis of SAP 155 expression in non-transfected: sc-117752 (A) and mouse SAP 155 transfected: sc-110269 (B) 293T whole cell lysates and Jurkat (C), K-562 (D), Ramos (E) and MCF7 (F) nuclear extracts.

SAP 155 (N-13) - sc-132447 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SAP 155 (N-13): sc-132447. Western blot analysis of SAP 155 expression in non-transfected: sc-117752 (A) and mouse SAP 155 transfected: sc-110269 (B) 293T whole cell lysates and Jurkat (C), K-562 (D), Ramos (E) and MCF7 (F) nuclear extracts.

SAP 155 (P-25) - sc-102102 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
SAP 155 (P-25): sc-102102. Western blot analysis of SAP 155 expression in Jurkat whole cell lysate.

SAP 155 (D-12) - sc-132445 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SAP 155 (D-12): sc-132445. Western blot analysis of SAP 155 expression in Jurkat (A), K-562 (B) and Ramos (C) nuclear extracts.

Anti-SAP155 antibody - ab39578 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SAP155 antibody (ab39578) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-SAP155 antibody - ab66774 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SAP155 with ab66774 at 4-8µg/ml. Arrows indicate positively labelled hepatocytes. Magnification: 400X.

Anti-SAP155 antibody - ab50866 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Ab50866, at 4 µg/ml, staining SAP155 in paraffin embedded hepatocytes of human liver tissue by Immunohistochemistry.

Anti-SAP155 antibody - ab21843 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-SAP155 antibody (ab21843) at 1/1000 dilutionLane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ugLane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug SecondaryRabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

Anti-SAP155 antibody - ab66664 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SAP155 antibody (ab66664) at 0.3125 µg/mlLane 1 : MarkerLane 2 : Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-SAP155 antibody - ab116457 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-SAP155 antibody (ab116457) at 1/100 dilution + CEM cell line lysates at 35 µg

Anti-SF3B1 antibody [EPR10036(2)] - ab172633 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10036(2)
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling SF3B1 with ab172633 at a 1/50 dilution. DAPI nuclear staining (blue).

Anti-SF3B1 antibody [EPR11986] - ab172634 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11986
All lanes : Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilutionLane 1 : HeLa lysateLane 2 : Jurkat lysateLane 3 : K562 lysateLane 4 : Ramos lysateLysates/proteins at 10 µg per lane.

Anti-SF3B1 antibody [EPR11987(B)] - ab170854 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR11987(B)
Immunofluorescent analysis of HeLa cells labeling SF3B1 with ab170854 at 1/100 dilution.

Anti-SF3B1 Antibody (aa373-402, AP) - LS-C217461 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SF3B1 Antibody (aa287-336) - LS-C110560 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SF3B1 antibody LS-C110560 Western blot of HeLa lysate.

Anti-SF3B1 Antibody (aa373-402, APC) - LS-C217462 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SF3B1 Antibody (aa373-402, Biotin) - LS-C217463 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SF3B1 Antibody (aa373-402, FITC) - LS-C217465 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SF3B1 Antibody (aa373-402, PE) - LS-C217466 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SF3B1 Antibody (aa134-179) - LS-C313940 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SF3B1 Antibody (aa373-402) - LS-C325030 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SF3B1 Antibody (clone 1A5) - LS-C179400 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1A5

Anti-SF3B1 Antibody (aa200-250) - LS-C287826 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human SF3b155/SAP155 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-SF3b155/SAP155 used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

Anti-SF3B1 Antibody (aa374-402) - LS-C169005 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SF3B1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human esophagus carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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