PAPOLA

Gene Symbol PAPOLA
Entrez Gene 10914
Alt Symbol PAP
Species Human
Gene Type protein-coding
Description poly(A) polymerase alpha
Other Description PAP-alpha|polynucleotide adenylyltransferase alpha
Swissprots Q9BVU2 Q86TV0 P51003 Q8IYF5 Q86SX4
Accessions EAW81642 EAW81643 EAW81644 EAW81645 EAW81646 EAW81647 EAW81648 EAW81649 P51003 AA236200 AF002990 AK024849 AK057629 AK097832 AK295020 BAG58079 AK302406 BAG63716 AK302984 BAG64124 AK303402 BAG64456 AK307659 AK307680 AK316168 BAH14539 BC000927 AAH00927 BC015150 BC036014 AAH36014 BX089817 BX161482 CAD61935 BX248301 CAD62628 BX248753 CAD66560 CD655865 DB045100 DB483858 X76770 XM_005267281 XP_005267338 XM_005267282 XP_005267339 NM_001252006 NP_001238935 NM_001252007 NP_001238936 NM_001293627 NP_001280556 NM_001293628 NP_001280557 NM_001293632 NP_001280561 NM_032632 NP_116021
Function Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
Subcellular Location Cytoplasm. Nucleus. Note=The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.
Top Pathways mRNA surveillance pathway

PAP-α/β/γ (D-1) - sc-365607 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PAP-α/β/γ (D-1): sc-365607. Western blot analysis of PAP-α expression in non-transfected: sc-110760 (A) and human PAP-α transfected: sc-110765 (B) 293 whole cell lysates.

PAP-α/β/γ (T-20) - sc-20188 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PAP (H-300) - sc-32915 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PAP (H-300): sc-32915. Western blot analysis of PAP expression in non-transfected: sc-110760 (A) and human PAP transfected: sc-110765 (B) 293 whole cell lysates.

Anti-PAPOLA antibody - ab126934 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab126934 stained JEG3 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126934 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-PAPOLA antibody - ab170443 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded Human prostate carcinoma tissue labeling PAPOLA with ab170443 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-PAPOLA antibody - ab72492 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAPOLA antibody (ab72492) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg

Anti-PAPOLA antibody - ab72426 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa525-575) - LS-C93033 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa600-650) - LS-C93034 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa407-435, AP) - LS-C265533 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa407-435, APC) - LS-C265534 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa407-435, FITC) - LS-C265535 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa407-435, Biotin) - LS-C265536 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa407-435, Azide-free, HRP) - LS-C265537 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa407-435, PE) - LS-C265538 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa525-575) - LS-C287838 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa600-650) - LS-C287839 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (C-Terminus) - LS-C287840 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa414-443) - LS-C323776 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PAPOLA Antibody (aa407-435) - LS-C163357 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PAP Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human prostate carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

PAPOLA Antibody - NBP1-87345 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: PAPOLA Antibody [NBP1-87345] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

PAPOLA Antibody - NB100-55268 from Novus Biologicals

Prices $369.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: PAPOLA Antibody [NB100-55268] - Detection of Human PAPOLA on HeLa whole cell lysate using NB100-55268. PAPOLA was also immunoprecipitated by rabbit anti-PAPOLA antibody NB100-55267.

PAPOLA Antibody - NB100-55267 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PAPOLA Antibody [NB100-55267] - Detection of Human PAPOLA on HeLa whole cell lysate using NB100-55267. PAPOLA was also immunoprecipitated by rabbit anti-PAPOLA antibody NB100-55268.

PAPOLA Antibody - NBP2-19691 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PAPOLA Antibody [NBP2-19691] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.

PAPOA Polyclonal Antibody - bs-4966R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat brain labeled with Anti-PAPOA Polyclonal Antibody, Unconjugated (bs-4966R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Search more