RAD50

Gene Symbol RAD50
Entrez Gene 10111
Alt Symbol NBSLD, RAD502, hRad50
Species Human
Gene Type protein-coding
Description RAD50 homolog (S. cerevisiae)
Other Description DNA repair protein RAD50
Swissprots Q9UP86 Q92878 Q6GMT7 B9EGF5 Q6P5X3 O43254
Accessions EAW62328 EAW62329 EAW62330 Q92878 AF057299 AAD50325 AF057300 AAD50326 AK290597 BAF83286 AK307439 BC062603 AAH62603 BC073850 AAH73850 BC108282 AAI08283 BC136436 AAI36437 BC140005 AAI40006 DB112912 DQ655928 U63139 AAB07119 NM_005732 NP_005723 NM_133482
Function Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. {ECO:0000269|PubMed:10888888, ECO:0000269|PubMed:11741547, ECO:0000269|PubMed:15064416, ECO:0000269|PubMed:9590181, ECO:0000269|PubMed:9651580, ECO:0000269|PubMed:9705271}.
Subcellular Location Nucleus. Chromosome, telomere. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents.
Tissue Specificity Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts. {ECO:0000269|PubMed:8756642}.
Top Pathways Homologous recombination, Non-homologous end-joining

Phospho-Rad50 (Ser635) Antibody - 14223 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blotting analysis of extracts from 293 cells, untreated (-) or UV-treated (100 mJ, 2 hr; +) or HCT 116 cells, untreated (-) or neocarzinostatin-treated (NCS, 10μM, 1 hr; +) using Phospho-Rad50 (Ser635) Antibody (upper) or Rad50 Antibody #3427 (lower).

Rad50 Antibody - 3427 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Jurkat and K562 cells, using RAD50 Antibody.

Rad50 (C-20) - sc-18291 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Rad50 (G-2) - sc-74460 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rad50 (G-2): sc-74460. Western blot analysis of Rad50 expression in Jurkat (A), c4 (B) and L8 (C) whole cell lysates.

Rad50 (H-300) - sc-20155 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rad50 (H-300): sc-20155. Immunofluorescence stain-ing of formalin-fixed HeLa cells showing nuclear and cytoplasmic localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

Rad50 (N-15) - sc-33519 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Rad50 (13B3/2C6) - sc-56209 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Rad50 (13B3/2C6): sc-56209. Western blot analysis of Rad50 expression in HeLa (A), K-562 (B), MCF7 (C), Ramos (D), SW480 (E) and Jurkat (F) nuclear extracts.

Anti-Rad50 antibody - ab84397 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Rad50 by Immunohistochemistry.Sample: FFPE section of Human larynx squamous cell carcinoma.Antibody: ab84397 used at a dilution of 1/250.Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1/100.

Anti-Rad50 antibody - ab3622 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Lanes:1. IP from 1mg extract with 1.25g Rabbit anti-Rad 50 (exon 2-3) (ab3622)2. IP from 1mg extract with 1.25µg Rabbit anti-Rad 50 (exon 13-14) (ab3623)Sample: Nuclear extract from Hela cells for Western blot, affinity purified Rabbit anti-Rad 50 (ab3622) used at 1:4000 dilution

Anti-Rad50 antibody - ab3623 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Rad50 with ab3623 at 1/1000 (1µg/ml). Detection: DAB.

Anti-Rad50 antibody - ab65134 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad50 antibody (ab65134) at 1/500 dilutionLane 1 : Extracts from COLO205 cellsLane 2 : Extracts from 293 cellsLane 3 : Extracts from HUVEC cellsLane 4 : Extracts from HUVEC cells, plus immunising peptide

Anti-Rad50 antibody - ab183084 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rad50 antibody (ab183084) at 1/900 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : mouse NIH 3T3 cell lysateLane 5 : mouse RAW264.7 cell lysateLysates/proteins at 40 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutiondeveloped using the ECL technique

Anti-Rad50 antibody [13B3/2C6] - ab89 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 13B3/2C6

Anti-Rad50 antibody [20B5] - ab87918 from Abcam

Prices $392.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 20B5
IHC image of Rad50 staining in Human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87918, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Rad50 antibody [20B5] - ab119708 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 20B5
All lanes : Anti-Rad50 antibody [20B5] (ab119708) at 1/2000 dilutionLane 1 : HeLa cell lysateLane 2 : SK-N-MC cell lysateLane 3 : A431 cell lysateLane 4 : HepG2 cell lysateLane 5 : SK-BR3 cell lysateLane 6 : Ramos cell lysateLane 7 : H9C2 cell lysateLane 8 : L929 cell lysate

Anti-Rad50 antibody [EPR3466(2)] - ab124682 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3466(2)
ab124682 staining Rad50 in the U2OS cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 5% BSA for 60 minutes. Samples were incubated with undiluted primary antibody  for 2 hours. An undiluted FITC conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.See Abreview

Anti-Rad50 antibody - N-terminal - ab182088 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Rad50 antibody - N-terminal (ab182088) at 1 µg/ml + PANC1 whole cell lysate at 10 µg

Anti-RAD50 Antibody (aa1-425) - LS-C17557 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-RAD50 Antibody - LS-C17558 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RAD50 Antibody - LS-C17561 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAD50 Antibody (N-Terminus) - LS-C38701 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAD50 Antibody (aa1-425, clone 4H9) - LS-C53371 from LifeSpan Bioscience

Prices $445.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 4H9

Anti-RAD50 Antibody (aa1-425) - LS-C83536 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-RAD50 Antibody (C-Terminus) - LS-C2861 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
RAD50 Antibody - WB detection of Rad50 in HeLa nuclear extracts.

Anti-RAD50 Antibody (aa628-787) - LS-C93574 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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