GAD2

Gene Symbol GAD2
Entrez Gene 2572
Alt Symbol GAD65
Species Human
Gene Type protein-coding
Description glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
Other Description 65 kDa glutamic acid decarboxylase|GAD-65|Glutamate decarboxylase-2 (pancreas)|glutamate decarboxylase 2|glutamate decarboxylase 65 kDa isoform
Swissprots Q05329 Q9UD87
Accessions AAP88040 EAW86101 EAW86102 EAW86103 EAW86104 Q05329 AJ251501 CAB62572 AK127375 BAC86947 BC029517 AAH29517 BC039038 BC047899 BC126327 AAI26328 BC126329 AAI26330 BI438818 BP392604 HQ258311 ADR83065 M74826 AAA58491 M81882 AAA62367 X69936 NM_000818 NP_000809 NM_001134366 NP_001127838
Function Catalyzes the production of GABA.
Subcellular Location Cytoplasm, cytosol {ECO:0000269|PubMed:12356867}. Cytoplasmic vesicle {ECO:0000269|PubMed:12356867}. Cell junction, synapse, presynaptic cell membrane {ECO:0000269|PubMed:12356867}; Lipid-anchor {ECO:0000269|PubMed:12356867}. Golgi apparatus membrane {ECO:0000269|PubMed:12356867}; Peripheral membrane protein {ECO:0000269|PubMed:12356867}; Cytoplasmic side {ECO:0000269|PubMed:12356867}. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
Top Pathways Type I diabetes mellitus, GABAergic synapse, Taurine and hypotaurine metabolism, Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism

GAD2 (D5G2) XP ® Rabbit mAb - 5843 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5G2
Western blot analysis of extracts from neonatal mouse and fetal rat brain using GAD2 (D5G2) XP ® Rabbit mAb.

GAD2 Antibody - 3988 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from mouse and rat brain using GAD2 Antibody.

GAD-65 (A-3) - sc-377145 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAD-65 (A-3): sc-377145. Western blot analysis of GAD-65 expression in rat cerebellum tissue extract (A) and U-87 MG whole cell lysate (B).

GAD-65 (B96) - sc-53541 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Human
Clonality Monoclonal
GAD-65 (B96): sc-53541. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.

GAD-65 (GAD-6) - sc-32270 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of GAD-65 expression in rat brain tissue extract immunoprecipitated with GAD-65/67 (C-20): sc-7513 and detected with GAD-65 (GAD-6): sc-32270.

GAD-65 (N-19) - sc-7511 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GAD-65 (N-19): sc-7511. Western blot analysis of GAD-65 expression in HeLa (A) and IMR-32 (B) nuclear extracts.

GAD-65 (H-95) - sc-5601 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GAD-65 Antibody (H-95): sc-5601. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing weak cytoplasmic staining of exocrine glandular cells and cytoplasmic staining of Islets of Langerhans and pancreatic duct cells.

GAD-65 (61/8F9) - sc-130569 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

GAD-65 (N-GAD65) - sc-73650 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAD-65 (N-GAD65): sc-73650. Western blot analysis of GAD-65 expression in rat cerebellum tissue extract.

Anti-GAD65 antibody - ab203063 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat testis tissue labeling GAD65 with ab203063 at 1/200 dilution.

Anti-GAD65 antibody - ab139958 from Abcam

Prices $376.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
IHC image of GAD65 staining in human brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139958, 1 µg/mL, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GAD65 antibody - ab112497 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GAD65 antibody - ab75687 from Abcam

Prices $376.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal
ab75687, at 1/50 dilution, staining GAD65 in Human pancreas by immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Anti-GAD65 antibody [144] - ab85866 from Abcam

Prices $388.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 144

Anti-GAD65 antibody [GAD-6] - ab26113 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone GAD-6
Anti-GAD65 antibody [GAD-6] (ab26113) at 1/1000 dilution + mouse cerebellum at 0.75 µgSecondaryHRP horse anti-mouse IgG at 1/5000 dilution

Anti-GAD65 antibody, prediluted - ab15299 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab15299 staining GAD65 in human pancreas by Immunohistochemistry (FFPE-sections).

Anti-GAD65 antibody - Neuronal Marker - ab2348 from Abcam

Prices $376.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, staining GAD65 with ab2348.

Anti-GAD65 Antibody (Internal, clone EPR2553Y) - LS-C105442 from LifeSpan Bioscience

Prices $480.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2553Y
Western blot analysis on (A) fetal brain, (B) mouse brain and (C) rat brain lysates using anti-GAD2 antibody (LS-C105442), dilution 1:1000.

Anti-GAD65 Antibody (N-Terminus) - LS-C203192 from LifeSpan Bioscience

Prices $245.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal

Anti-GAD65 Antibody (Internal) - LS-C210107 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-GAD65 Antibody - LS-C137021 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GAD65 Antibody (aa109-138, AP) - LS-C214557 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAD65 Antibody (aa109-138) - LS-C159700 from LifeSpan Bioscience

Prices $295.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
GAD2 Antibody western blot of rat brain tissue lysates (35 ug/lane). The GAD2 antibody detected the GAD2 protein (arrow).

Anti-GAD65 Antibody (aa109-138, APC) - LS-C214558 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAD65 Antibody (aa109-138, Biotin) - LS-C214559 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more