NUDT16
| Gene Symbol | NUDT16 |
|---|---|
| Entrez Gene | 131870 |
| Alt Symbol | - |
| Species | Human |
| Gene Type | protein-coding |
| Description | nudix (nucleoside diphosphate linked moiety X)-type motif 16 |
| Other Description | IDP phosphatase|IDPase|U8 snoRNA-binding protein H29K|U8 snoRNA-decapping enzyme|inosine diphosphate phosphatase|m7GpppN-mRNA hydrolase|nucleoside diphosphate-linked moiety X motif 16|nudix motif 16 |
| Swissprots | E9PED4 Q96N82 Q96DE0 B4E3B4 F5GYJ1 |
| Accessions | EAW79210 Q96DE0 AK055827 BAB71024 AK091353 AK093567 AK295852 BAG58658 AK304650 BAG65426 BC009546 AAH09546 BC031215 AAH31215 BP199028 BP219080 CB121755 DC343245 NM_001171905 NP_001165376 NM_001171906 NP_001165377 NM_152395 NP_689608 NR_033268 |
| Function | RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal- dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxygu |
| Subcellular Location | Nucleus. Nucleus, nucleoplasm {ECO:0000250}. Nucleus, nucleolus. Cytoplasm. Note=Localized predominantly in the cytoplasm. Localized in nucleolus, and in a minor proportion in distinct foci in the nucleoplasm (By similarity). {ECO:0000250}. |
| Tissue Specificity | Expressed strongly in lung, kidney, adrenal gland, testis, heart and brain. {ECO:0000269|PubMed:20385596}. |
| Top Pathways | RNA degradation, Purine metabolism |
NUDT16 CRISPR/Cas9 KO Plasmid (h) - sc-414385 from Santa Cruz Biotechnology
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NUDT16 HDR Plasmid (h) - sc-414385-HDR from Santa Cruz Biotechnology
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NUDT16 Double Nickase Plasmid (h) - sc-414385-NIC from Santa Cruz Biotechnology
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NUDT16 Double Nickase Plasmid (h2) - sc-414385-NIC-2 from Santa Cruz Biotechnology
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NUDT16 CRISPR Activation Plasmid (h) - sc-414385-ACT from Santa Cruz Biotechnology
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NUDT16 CRISPR Activation Plasmid (h2) - sc-414385-ACT-2 from Santa Cruz Biotechnology
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NUDT16 Lentiviral Activation Particles (h) - sc-414385-LAC from Santa Cruz Biotechnology
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NUDT16 Lentiviral Activation Particles (h2) - sc-414385-LAC-2 from Santa Cruz Biotechnology
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NUDT16 siRNA (h) - sc-78514 from Santa Cruz Biotechnology
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NUDT16 shRNA Plasmid (h) - sc-78514-SH from Santa Cruz Biotechnology
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NUDT16 shRNA (h) Lentiviral Particles - sc-78514-V from Santa Cruz Biotechnology
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NUDT16 RNAi - H00131870-R01 from Novus Biologicals
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MISSION® esiRNA - EHU050241 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN15594 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND15594 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR11753 from Sigma-Aldrich
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NUDT16 siRNA (Human) - CRJ5075 from Cohesion Biosciences
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shRNA set against Human NUDT16 (NM_152395.2) - SHH356084 from Creative biogene
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NUDT16 shRNA Plasmid (Human) - CHJ5075 from Cohesion Biosciences
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