GAK

Gene Symbol GAK
Entrez Gene 2580
Alt Symbol DNAJ26, DNAJC26
Species Human
Gene Type protein-coding
Description cyclin G associated kinase
Other Description auxilin-2|cyclin-G-associated kinase
Swissprots Q5U4P5 Q9BVY6 O14976
Accessions EAW82644 EAW82645 EAW82646 O14976 AB208854 BAD92091 AK092587 AK097714 BAG53518 AK126735 BAC86662 AK127351 AK131464 BAD18609 AK299554 BAG61499 AK309413 BC000816 AAH00816 BC001290 BC008668 AAH08668 BC063606 AAH63606 BC085005 AAH85005 BC107076 AAI07077 BT007015 AAP35661 D88435 BAA22623 DA680073 XM_005272268 XP_005272325 XM_005272270 XP_005272327 XM_011513425 XP_011511727 XM_011513426 XP_011511728 XM_011513427 XP_011511729 XM_011513428 XP_011511730 XM_011513429 XP_011511731 XM_011513430 XP_011511732 XM_011513431 XP_011511733 XM_011513432 XP_011511734 XM_011513433 XP_011511735 XM_011513434 XP_011511736 NM_001286833 NP_001273762 NM_005255 NP_005246
Function Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. {ECO:0000269|PubMed:10625686}.
Subcellular Location Cytoplasm, perinuclear region {ECO:0000269|PubMed:10625686}. Golgi apparatus, trans-Golgi network {ECO:0000269|PubMed:10625686}. Cell junction, focal adhesion {ECO:0000305|PubMed:10625686}. Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
Tissue Specificity Ubiquitous. Highest in testis.

GAK (D-2) - sc-137053 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAK (D-2): sc-137053. Western blot analysis of GAK expression in non-transfected: sc-117752 (A) and truncated human GAK transfected: sc-111171 (B) 293T whole cell lysates.

GAK (G-10) - sc-137066 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAK (G-10): sc-137066. Immunofluorescence staining of methanol-fixed HeLa cells showing showing cytoplasmic and perinuclear staining.

GAK (R-360) - sc-7864 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GAK (R-360): sc-7864. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells.

Anti-GAK antibody - ab190231 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GAK antibody (ab190231) at 1/500 dilutionLane 1 : 293 cell lysateLane 2 : 293 cell lysate with synthesized peptide

Anti-GAK antibody [1C2] - ab115179 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C2
All lanes : Anti-GAK antibody [1C2] (ab115179) at 1 µg/mlLane 1 : Jurkat cell lysateLane 2 : Raji cell lysateLane 3 : MCF7 cell lysateLane 4 : U937 cell lysateLane 5 : HL60 cell lysateLane 6 : HepG2 cell lysateLane 7 : WR19L12a cell lysateLane 8 : NIH3T3 cell lysate

Anti-GAK antibody - C-terminal - ab135683 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GAK antibody - C-terminal (ab135683) at 1/100 dilutionLane 1 : 293 cell lysate, non-transfectedLane 2 : 293 cell lysate, transiently transfected with the GAK geneLysates/proteins at 2 µg per lane.

Anti-GAK protein antibody - ab86397 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-GAK protein antibody (ab86397) at 1/100 dilution + mouse heart tissue lysate at 12.5 µg

Anti-GAK Antibody (Gln129) - LS-C191920 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (clone 1C2) - LS-C179789 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C2

Anti-GAK Antibody (C-Terminus, AP) - LS-C277477 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (C-Terminus, APC) - LS-C277478 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (C-Terminus, FITC) - LS-C277480 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (C-Terminus, Biotin) - LS-C277479 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (C-Terminus, Azide-free, HRP) - LS-C277481 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (C-Terminus, PE) - LS-C277482 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (N-Terminus, AP) - LS-C277484 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (N-Terminus, APC) - LS-C277483 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (N-Terminus, Biotin) - LS-C277485 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (N-Terminus, PE) - LS-C277489 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (N-Terminus, FITC) - LS-C277486 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (N-Terminus, Azide-free, HRP) - LS-C277490 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody - LS-C310430 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody (aa80-129) - LS-C291974 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GAK Antibody - LS-C307635 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-GAK Antibody (aa15-45) - LS-C101154 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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