RAP2A

Gene Symbol RAP2A
Entrez Gene 5911
Alt Symbol K-REV, KREV, RAP2, RbBP-30
Species Human
Gene Type protein-coding
Description RAP2A, member of RAS oncogene family
Other Description RAP2, member of RAS oncogene family (K-rev)|ras-related protein Rap-2a
Swissprots P10114 Q5JSC1 Q5JSC2 B2RCJ1
Accessions CAA00806 EAX08974 P10114 AF205602 AAN71845 AF493914 AAM12628 AK090533 AK094586 AK311202 AK315139 BAG37588 BC022356 BC041333 AAH41333 BC047495 BC070031 AAH70031 CB215818 DA493957 DB480985 EU832309 ACE87566 EU832393 ACE86878 X12534 CAA31052 NM_021033 NP_066361
Function Small GTP-binding protein which cycles between a GDP- bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading. {ECO:0000269|PubMed:14966141, ECO:0000269|PubMed:15342639, ECO:0000269|PubMed:16246175, ECO:0000269|PubMed:16540189, ECO:0000269|PubMed:18930710, ECO:0000269|PubMed:20159449}.
Subcellular Location Recycling endosome membrane; Lipid-anchor; Cytoplasmic side. Midbody. Note=May also localize to the Golgi (PubMed:7962206) and the gelatinase-containing granules of neutrophils (PubMed:8391995). Colocalizes with RASGEF1B to midbody at telophase (PubMed:23894443). {ECO:0000269|PubMed:23894443, ECO:0000269|PubMed:7962206, ECO:0000269|PubMed:8391995}.

Rap 2 (124) - sc-164 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rap 2 (124): sc-164. Western blot analysis of Rap 2C expression in non-transfected 293T: sc-117752 (A), mouse Rap 2C transfected 293T: sc-122970 (B) and A-431 (C) whole cell lysates.

Rap 2 (FL-183) - sc-292458 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rap 2 (FL-183): sc-292458. Western blot analysis of Rap 2C expression in non-transfected: sc-117752 (A) and mouse Rap 2C transfected: sc-122971 (B) 293T whole cell lysates.

Rap 2 (V-19) - sc-1062 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rap 2 (V-19): sc-1062. Immunofluorescence staining of methanol-fixed A-431 cells showing membrane staining.

Rap 2 (12) - sc-136138 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Rap 2 (12): sc-136138. Western blot analysis of Rap 2 expression in A-431 whole cell lysate.

Anti-Rap2A antibody - ab49685 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Rap2A antibody (ab49685) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-RAP2 Antibody (aa140-190) - LS-C144134 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RAP2 Antibody (aa99-148) - LS-C151600 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RAP2 antibody Western Blot of MCF7.

Anti-RAP2 Antibody - LS-C335261 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of K562 cell line, using RAP2A antibody.

Anti-RAP2 Antibody (aa31-80) - LS-C151601 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RAP2 antibody Western Blot of 721_B lysate. Antibody Dilution: 1.0 ug/ml. RAP2A is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells. Antibody dilution: 1 ug/ml.

Anti-RAP2 Antibody (aa140-190) IHC-plus™ - LS-B7974 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RAP2 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7974 dilution 10 ug/ml.

Rap2A Antibody - NBP2-24574 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Rap2A Antibody [NBP2-24574] - analysis of RAP2A in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using RAP2A antibody at 0.1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Rap2A/B Antibody (455410) - MAB4698 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 455410
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat Rap2A/B Monoclonal Antibody (Catalog # MAB4698) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Rap2A and Rap2B (5 ng/lane) were included. A specific band for Rap2A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Rap2A Antibody - NBP1-98405 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

Rap2A Antibody - NBP1-98483 from Novus Biologicals

Prices $369.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western Blot: Rap2A Antibody [NBP1-98483] - Hela Cell Lysate 1.0ug/ml, Gel Concentration: 12%

Rap2A Antibody - NBP2-20074 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: Rap2A Antibody [NBP2-20074] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.

Rap2A Antibody - H00005911-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: Rap2A Antibody [H00005911-A01] - analysis of RAP2A expression in transfected 293T cell line by RAP2A polyclonal antibody (A01). Lane1: RAP2A transfected lysate (20.6 KDa). Lane2: Non-transfected lysate.

Rabbit Anti-Rap2A Polyclonal Antibody - bs-9872R from Bioss

Prices $245.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAP2A antibody produced in rabbit - SAB2700406 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-RAP2A: <B>Cat. No. SAB2700406</B>: Immunofluorescence analysis of methanol-fixed MCF-7, using RAP2A antibody at 1:500 dilution.

Anti-RAP2A antibody produced in rabbit - SAB2107824 from Sigma-Aldrich

Prices $341.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RAP2A: <B>Cat. No. SAB2107824</B>: Western blot analysis of RAP2A with RAP2A (RAP2A, member of RAS oncogene family) antibody against the N-terminal of RAP2A (50 μg) antibody at 1.0 μg/mL.

Anti-RAP2A (146-156) antibody produced in rabbit - SAB1104797 from Sigma-Aldrich

Prices $285.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human RAP2A was separated on SDS-PAGE, blotted with Anti-RAP2A (146-156) (<B>Cat. No. SAB1104797</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000<BR/>2. Antibody dilution 1:1,000 + RAP2A immunizing peptide (human, 146-156)

Anti-RAP2A antibody produced in rabbit - SAB2107823 from Sigma-Aldrich

Prices $341.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RAP2A: <B>Cat. No. SAB2107823</B>: Western blot analysis of RAP2A with RAP2A (RAP2A, member of RAS oncogene family) antibody against the C-terminal of RAP2A (50 μg) antibody at 1.0 μg/mL.

RAP2A antibody - C-terminal region (ARP64032_P050) - ARP64032_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RAP2A antibody - N-terminal region (ARP64033_P050) - ARP64033_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Human/Mouse/Rat Rap2A/B Antibody - MAB4698 from R&D Systems

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clone 455410
Detection of Human, Mouse, and Rat Rap2A/B by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat Rap2A/B Monoclonal Antibody (Catalog # MAB4698) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). For additional reference, recombinant human Rap2A and Rap2B (5 ng/lane) were included. A specific band for Rap2A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3 .

Human/Mouse/Rat Rap2A/B MAb (Clone 455410), Mouse IgG2B - MAB4698-SP from R&D Systems

Host Mouse
Clonality Monoclonal
Clone 455410
Detection of Human, Mouse, and Rat Rap2A/B by Western Blot.Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat Rap2A/B Monoclonal Antibody (Catalog # MAB4698) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Rap2A and Rap2B (5 ng/lane) were included. A specific band for Rap2A/B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
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