PTK6

Gene Symbol PTK6
Entrez Gene 5753
Alt Symbol BRK
Species Human
Gene Type protein-coding
Description protein tyrosine kinase 6
Other Description PTK6 protein tyrosine kinase 6|breast tumor kinase|protein-tyrosine kinase 6|protein-tyrosine kinase BRK|tyrosine-protein kinase BRK
Swissprots Q13882 Q58F01 B2RCR3 B4DW46
Accessions AAC34935 EAW75262 EAW75263 Q13882 AK124082 AK301364 BAG62908 AK301691 BAG63163 AK315232 BAG37660 BC035843 AAH35843 DA762278 X78549 CAA55295 XR_936592 NM_001256358 NP_001243287 NM_005975 NP_005966
Function Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells
Subcellular Location Cytoplasm. Nucleus. Cell projection, ruffle. Membrane {ECO:0000250}. Note=Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. Nuclear localization in epithelial cells of normal prostate but cytoplasmic localization in cancer prostate.
Tissue Specificity Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Not expressed in breast or ovarian tissue but expressed in high percentage of breast and ovarian cancers. Also overexpressed in some metastatic melanomas, lymphomas, colon cancers, squamous cell carcinomas and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 2 is present in prostate epithelial cell lines derived from normal prostate and prostate adenocarcinomas, as well as in a variety of cell lines. {ECO:0000269|PubMed:12833144, ECO:0000269|PubMed:15509496, ECO:0000269|PubMed:16651629, ECO:0000269|PubMed:9185712}.

Brk (C-18) - sc-1188 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Brk (C-18): sc-1188. Western blot analysis of Brk expression in T-47D (A), SW480 (B), T24 (C) and ZR-75-1 (D) whole cell lysates.

Brk (N-20) - sc-915 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Brk (N-20): sc-915. Western blot analysis of Brk expression in non-transfected: sc-117752 (A) and human Brk transfected: sc-112582 (B) 293T whole cell lysates.

Brk (5G1) - sc-66003 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Brk (5G1): sc-66003. Western blot analysis of Brk expression in OV-90 (A), HuT 78 (B), FCH (C) and MCF7 (D) whole cell lysates.

Brk (D-7) - sc-137045 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Brk (D-7): sc-137045. Western blot analysis of truncated human recombinant Brk fusion protein.

Brk (G-6) - sc-166171 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Brk (G-6): sc-166171. Western blot analysis of Brk expression in non-transfected: sc-117752 (A) and human Brk transfected: sc-112582 (B) 293T whole cell lysates.

Brk (D-6) - sc-166243 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Brk (D-6): sc-166243. Western blot analysis of Brk expression in non-transfected: sc-117752 (A) and human Brk transfected: sc-112582 (B) 293T whole cell lysates.

Anti-Brk (phospho Y447) antibody - ab138368 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Brk (phospho Y447) antibody (ab138368) at 1/500 dilution + 293 cell extract treated with EGF 200ng/ml for 30 min at 30 µg

Anti-Brk antibody - ab71515 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Brk antibody (ab71515) at 0.5 µg/mlLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysateLane 3 : 3T3 cell lysateLane 4 : Rat kidney cell lysate

Anti-Brk antibody - ab65221 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Brk antibody (ab65221) at 1/500 dilutionLane 1 : LOVO cell extractLane 2 : LOVO cell extract with immunising peptide at 10 µgLysates/proteins at 10 µg per lane.

Anti-Brk antibody - ab137563 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Brk antibody (ab137563) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-Brk antibody [2H12B8] - ab52273 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2H12B8
All lanes : Anti-Brk antibody [2H12B8] (ab52273) at 1/2000 dilutionLane 1 : Cell lysates prepared from Hela cellsLane 2 : Cell lysates prepared from A549 cellsLane 3 : Cell lysates prepared from MCF-7 cellsLysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG

Anti-Brk antibody - N-terminal - ab135811 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Brk antibody - N-terminal (ab135811) at 1/50 dilutionLane 1 : A375 cell line lysate Lane 2 : A375 cell line lysate Lysates/proteins at 35 µg per lane.

Anti-PTK6 / BRK Antibody (N-Terminus) - LS-C35197 from LifeSpan Bioscience

Prices $530.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PTK6 / BRK Antibody - LS-C77242 from LifeSpan Bioscience

Prices $635.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-PTK6 / BRK Antibody (clone 2F11) - LS-C133934 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2F11

Anti-PTK6 / BRK Antibody - LS-C146987 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PTK6 / BRK Antibody (aa402-451) - LS-C117803 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of NIH-3T3 cells, using Breast Tumor Kinase (Ab-447) Antibody. The picture on the right is treated with the synthesized peptide.

Anti-PTK6 / BRK Antibody (aa435-451) - LS-C188881 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PTK6 / BRK Antibody (aa508) - LS-C190176 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PTK6 / BRK Antibody (clone 2H12B8) - LS-C47211 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2H12B8

Anti-PTK6 / BRK Antibody (aa1-30, AP) - LS-C267514 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PTK6 / BRK Antibody (phospho-Tyr342) - LS-C152797 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
WB: IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk (Y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(Y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(Y342).

Anti-PTK6 / BRK Antibody (aa1-30, APC) - LS-C267515 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PTK6 / BRK Antibody (aa1-30, Biotin) - LS-C267516 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PTK6 / BRK Antibody (aa1-30, FITC) - LS-C267517 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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