| Gene Symbol | ANKRD27 |
|---|---|
| Entrez Gene | 84079 |
| Alt Symbol | PP12899, VARP |
| Species | Human |
| Gene Type | protein-coding |
| Description | ankyrin repeat domain 27 (VPS9 domain) |
| Other Description | VPS9 domain-containing protein|VPS9-ankyrin-repeat protein|Vps9 domain and ankyrin-repeat-containing protein|ankyrin repeat domain-containing protein 27 |
| Swissprots | Q96NW4 Q9H0I4 Q86UC3 Q8ND80 Q71MF5 |
| Accessions | Q96NW4 AA903176 AF447882 AAQ04657 AK054561 BAB70755 AL136784 CAB66718 AL834335 CAD39003 AM393863 CAL38738 BC015552 BC045605 BC050529 AAH50529 BI462844 XM_005259310 XP_005259367 XM_006723412 XP_006723475 NM_032139 NP_115515 |
| Function | May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (PubMed:16525121, PubMed:18477474). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (PubMed:23104059). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (By similarity). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (By similarity). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1 (PubMed:22705394). Required for the colocalization of VAMP7 and Rab21, probably on TGN sites |
| Subcellular Location | Early endosome {ECO:0000269|PubMed:16525121, ECO:0000269|PubMed:24856514}. Late endosome {ECO:0000269|PubMed:23104059}. Cytoplasmic vesicle membrane {ECO:0000269|PubMed:23104059}. Lysosome {ECO:0000269|PubMed:23104059}. Cell membrane {ECO:0000269|PubMed:23104059}. Melanosome {ECO:0000250|UniProtKB:Q3UMR0}. Note=Colocalizes with VAMP7 in transport vesicles in the shaft of hippoocampal neurons (By similarity). {ECO:0000250|UniProtKB:Q3UMR0}. |
ANKRD27 CRISPR/Cas9 KO Plasmid (h) - sc-413549 from Santa Cruz Biotechnology
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ANKRD27 HDR Plasmid (h) - sc-413549-HDR from Santa Cruz Biotechnology
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ANKRD27 Double Nickase Plasmid (h) - sc-413549-NIC from Santa Cruz Biotechnology
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ANKRD27 Double Nickase Plasmid (h2) - sc-413549-NIC-2 from Santa Cruz Biotechnology
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ANKRD27 CRISPR Activation Plasmid (h) - sc-413549-ACT from Santa Cruz Biotechnology
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ANKRD27 CRISPR Activation Plasmid (h2) - sc-413549-ACT-2 from Santa Cruz Biotechnology
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ANKRD27 Lentiviral Activation Particles (h) - sc-413549-LAC from Santa Cruz Biotechnology
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ANKRD27 Lentiviral Activation Particles (h2) - sc-413549-LAC-2 from Santa Cruz Biotechnology
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ANKRD27 siRNA (h) - sc-97420 from Santa Cruz Biotechnology
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ANKRD27 shRNA Plasmid (h) - sc-97420-SH from Santa Cruz Biotechnology
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ANKRD27 shRNA (h) Lentiviral Particles - sc-97420-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU017751 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3194 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3194 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR08384 from Sigma-Aldrich
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ANKRD27 siRNA (Human) - CRJ3292 from Cohesion Biosciences
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ANKRD27 - MBS8229189 from MyBioSource
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shRNA set against Human ANKRD27 (NM_032139.2) - SHH236750 from Creative biogene
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shRNA set against Human ANKRD27(NM_032139.2) - SHG060085 from Creative biogene
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ANKRD27 shRNA Plasmid (Human) - CHJ3292 from Cohesion Biosciences
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ANKRD27 miRNA 3'UTR clone - MiUTR1H-00392 from Creative biogene
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