LARGE

Gene Symbol LARGE
Entrez Gene 9215
Alt Symbol MDC1D, MDDGA6, MDDGB6
Species Human
Gene Type protein-coding
Description like-glycosyltransferase
Other Description acetylglucosaminyltransferase-like 1A|acetylglucosaminyltransferase-like protein|glycosyltransferase-like protein LARGE1
Swissprots Q17R80 Q9UGG3 Q9UGE7 O95461 B0QXZ7 Q9UGD1 Q9UH22 O60348 Q9UGZ8
Accessions CAI17890 CAQ09433 CAQ09434 CAQ09435 CAX15214 CAX15215 EAW60041 EAW60042 EAW60043 O95461 AB011181 BAA25535 AI167885 AI198348 AI199197 AJ007583 CAA07571 AK294766 BAH11875 AK295636 BAH12135 AK301808 BAH13559 AK310462 BC117425 AAI17426 BC126404 AAI26405 BF057493 CR456510 CAG30396 CU013109 CAK54540 CU013397 CAK54839 DR000293 HQ258512 ADR83266 KJ534877 AHW56517 KJ535033 AHW56672 KJ535073 AHW56712 KJ535074 AHW56713 XM_005261831 XP_005261888 XM_005261832 XP_005261889 XM_011530510 XP_011528812 XM_011530511 XP_011528813 XM_011530512 XP_011528814 XM_011530513 XP_011528815 XM_011530514 XP_011528816 NM_004737 NP_004728 NM_133642 NP_598397
Function Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine- beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:22223806). Phosphorylated O- mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:25279699). {ECO:0000269|PubMed:15661757, ECO:0000269|PubMed:15752776, ECO:0000269|PubMed:21987822, ECO:0000269|PubMed:22223806, ECO:0000269|PubMed:25138275, ECO:0000269|PubMed:25279697, ECO:0000269|PubMed:25279699}.
Subcellular Location Golgi apparatus membrane {ECO:0000269|PubMed:15661757, ECO:0000269|PubMed:15958417, ECO:0000269|PubMed:25279699}; Single-pass type II membrane protein {ECO:0000269|PubMed:15661757, ECO:0000269|PubMed:15958417}.
Tissue Specificity Ubiquitous. Highest expression in heart, brain and skeletal muscle. {ECO:0000269|PubMed:15752776}.

LARGE (C-17) - sc-46342 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LARGE (C-17): sc-46342. Western blot analysis of LARGE expression in NIH/3T3 whole cell lysate.

LARGE (Y-14) - sc-33435 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LARGE (Y-14): sc-33435. Western blot analysis of LARGE expression in C2C12 whole cell lysate.

LARGE (H-164) - sc-99164 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-LARGE antibody - C-terminal - ab187728 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LARGE antibody - C-terminal (ab187728) at 1/1000 dilutionLane 1 : Mouse thymus total cell extractLane 2 : HeLa total cell extractLane 3 : Jurkat total cell extract

Anti-LARGE Antibody (aa53-66) - LS-C112979 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-LARGE Antibody (aa683-732) - LS-C111103 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
LARGE antibody LS-C111103 Western blot of HepG2 cell lysate.

Anti-LARGE Antibody (aa421-433) - LS-C112978 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
LARGE antibody (1 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

LARGE Antibody - NBP1-59946 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

LARGE Antibody - NBP1-82983 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: LARGE Antibody [NBP1-82983] - Staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.

LARGE Antibody - NBP1-49865 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: LARGE Antibody [NBP1-49865] - Analysis of LARGE (aa421-433) in Human Kidney lysate (35ug protein in RIPA buffer) using NBP1-49865 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

LARGE Antibody - NBP1-49887 from Novus Biologicals

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-LARGE antibody produced in rabbit - SAB2105671 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-LARGE: <B>Cat. No. SAB2105671</B>: LARGE in HepG2 cell lysates with LARGE antibody at 1.0 μg/mL.

Anti-LARGE antibody produced in goat - SAB2501652 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-LARGE <B>Cat. No. SAB2501652</B>: (1 μg/mL) staining of Human Kidney lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

LARGE Antibody (OAEE00484) - OAEE00484 from Aviva Systems Biology

Prices $230.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone LARGE-02

LARGE antibody - middle region (ARP46489_P050) - ARP46489_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

LARGE-N-terminus-Antibody-N-Term-near - AF3174a from Abgent, a WuXi AppTec company

Prices $325.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

LARGE-aa421-433-Antibody-internal-region - AF3173a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF3173a (1 µg/ml) staining of Human Kidney lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP2B1 antibody - GTX112479 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-AP2B1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX112479

gamma Adaptin antibody [N1N3] - GTX114244 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-gamma Adaptin antibody [N1N3] used in Western blot. GTX114244

LARGE (aa421-433) antibody, Internal - GTX88124 from GeneTex

Prices $319.00
Sizes 100 μg
Host Goat
Clonality Polyclonal
Anti-LARGE (aa421-433) antibody, Internal used in Western blot. GTX88124

LARGE (N-terminus) antibody, N-term - GTX88123 from GeneTex

Prices $239.00
Sizes 100 μg
Host Goat
Clonality Polyclonal

LARGE antibody - orb20534 from Biorbyt

Prices $408.00
Sizes 100 μg
Host Goat
Clonality Polyclonal
orb20534 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

LARGE antibody - N-terminal region (OAEB00400) - OAEB00400 from Aviva Systems Biology

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

LARGE Antibody (OAEB01526) - OAEB01526 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

LARGE antibody - 70R-6856 from Fitzgerald

Host Rabbit
Clonality Polyclonal
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