Gene Symbol | NDFIP1 |
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Entrez Gene | 80762 |
Alt Symbol | N4WBP5 |
Species | Human |
Gene Type | protein-coding |
Description | Nedd4 family interacting protein 1 |
Other Description | NEDD4 family-interacting protein 1|Nedd4 WW domain-binding protein 5|breast cancer-associated protein SGA-1M|putative MAPK-activating protein PM13|putative NF-kappa-B-activating protein 164|putative NFKB and MAPK-activating protein |
Swissprots | Q8N2F9 B2RDB8 D3DQF0 Q9BT67 Q8N2E3 Q658T8 |
Accessions | EAW61888 EAW61889 Q9BT67 AA496123 AB097010 BAC77363 AB097037 BAC77390 AI929639 AK075495 BAC11652 AK075524 BAC11670 AK124884 AK315481 BAG37865 AL832993 CAH56294 AY192728 AAP13460 BC004317 AAH04317 BX411356 DA236494 HQ447371 ADQ31857 NM_030571 NP_085048 |
Function | Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate. {ECO:0000250, ECO:0000269|PubMed:19343052, ECO:0000269|PubMed:19706893, ECO:0000269|PubMed:20534535}. |
Subcellular Location | Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane {ECO:0000250}. Secreted. Note=Detected in exosomes and secreted via the exosomal pathway. |
Tissue Specificity | Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung. {ECO:0000269|PubMed:11748237}. |
Ndfip1 CRISPR/Cas9 KO Plasmid (h) - sc-403470 from Santa Cruz Biotechnology
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Ndfip1 HDR Plasmid (h) - sc-403470-HDR from Santa Cruz Biotechnology
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Ndfip1 Double Nickase Plasmid (h) - sc-403470-NIC from Santa Cruz Biotechnology
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Ndfip1 Double Nickase Plasmid (h2) - sc-403470-NIC-2 from Santa Cruz Biotechnology
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Ndfip1 CRISPR Activation Plasmid (h) - sc-403470-ACT from Santa Cruz Biotechnology
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Ndfip1 CRISPR Activation Plasmid (h2) - sc-403470-ACT-2 from Santa Cruz Biotechnology
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Ndfip1 Lentiviral Activation Particles (h) - sc-403470-LAC from Santa Cruz Biotechnology
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Ndfip1 Lentiviral Activation Particles (h2) - sc-403470-LAC-2 from Santa Cruz Biotechnology
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Ndfip1 siRNA (h) - sc-75886 from Santa Cruz Biotechnology
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Ndfip1 shRNA Plasmid (h) - sc-75886-SH from Santa Cruz Biotechnology
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Ndfip1 shRNA (h) Lentiviral Particles - sc-75886-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN15120 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND15120 from Sigma-Aldrich
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NDFIP1 siRNA (Human) - CRJ2905 from Cohesion Biosciences
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shRNA set against Human NDFIP1 (NM_030571.3) - SHH350288 from Creative biogene
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Human NDFIP1 siRNA - orb273406 from Biorbyt
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NDFIP1 shRNA Plasmid (Human) - CHJ2905 from Cohesion Biosciences
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NDFIP1 miRNA 3'UTR clone - MiUTR4H-TG05901 from Creative biogene
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