TIMM50

Gene Symbol TIMM50
Entrez Gene 92609
Alt Symbol TIM50, TIM50L
Species Human
Gene Type protein-coding
Description translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae)
Other Description Tim50-like protein|homolog of yeast Tim50|mitochondrial import inner membrane translocase subunit TIM50
Swissprots Q9H370 Q96FJ5 Q96GY2 Q3ZCQ8 Q330K1 Q6QA00
Accessions EAW56901 EAW56902 EAW56903 Q3ZCQ8 AK092396 AK097291 AK098197 AY444561 AAS68537 AY551341 AAT01208 BC009072 AAH09072 BC010736 AAH10736 BC014674 BC050082 AAH50082 BC121146 AAI21147 BC121147 AAI21148 BE386959 BI856163 BQ925812 BU540967 XM_011527491 XP_011525793 NM_001001563 NP_001001563
Function Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. {ECO:0000269|PubMed:15044455}.
Subcellular Location Mitochondrion inner membrane {ECO:0000269|PubMed:15044455, ECO:0000269|PubMed:16008839}; Single-pass membrane protein {ECO:0000269|PubMed:15044455, ECO:0000269|PubMed:16008839}.
Tissue Specificity Widely expressed. Expressed at higher level in brain, kidney and liver (at protein level). {ECO:0000269|PubMed:15044455, ECO:0000269|PubMed:16008839}.

Tim50 (C-9) - sc-393678 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Tim50 (C-9): sc-393678. Western blot analysis of Tim50 expression in T98G (A), Caki-1 (B) and MDA-MB-231 (C) whole cell lysates and rat heart (D) and human heart (E) tissue extracts. Note lack of reactivity in lane D.

Tim50 (G-20) - sc-55338 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Tim50 (G-20): sc-55338. Western blot analysis of Tim50 expression in rat heart tissue extract.

Tim50 (H-300) - sc-367629 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Tim50 (S-19) - sc-55340 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Tim50 (S-19): sc-55340. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells and Interstitial cells.

Anti-TIMM50 antibody - ab23938 from Abcam

Prices $391.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-TIMM50 antibody (ab23938) at 0.5 µg/mlPerformed under reducing conditions.

Anti-TIMM50 antibody [EPR5784] - ab109527 from Abcam

Prices $99.00, $385.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5784
All lanes : Anti-TIMM50 antibody [EPR5784] (ab109527) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : SH SY5Y cell lysateLane 3 : HepG2 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-TIMM50 antibody [EPR5785] - ab109436 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5785
All lanes : Anti-TIMM50 antibody [EPR5785] (ab109436) at 1/1000 dilutionLane 1 : 293T cell lysate Lane 2 : SH-SY5Y cell lysateLane 3 : HepG2 cell lysate Lane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.

Anti-TIMM50 antibody - C-terminal - ab177353 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-TIMM50 antibody - C-terminal (ab177353) at 1 µg/ml + HepG2 whole cell lysate at 10 µg

Anti-TIMM50 antibody - N-terminal - ab138772 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-TIMM50 antibody - N-terminal (ab138772) at 1/100 dilution + MDA-MB453 cell line lysates at 35 µg

Anti-TIMM50 Antibody (aa74-104, AP) - LS-C271512 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMM50 Antibody (aa74-104, APC) - LS-C271513 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMM50 Antibody (aa74-104, Biotin) - LS-C271514 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMM50 Antibody (aa74-104, FITC) - LS-C271515 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMM50 Antibody (aa74-104, Azide-free, HRP) - LS-C271516 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMM50 Antibody (aa74-104, PE) - LS-C271517 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMM50 Antibody (aa74-104) - LS-C325733 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TIMM50 Antibody (Internal, clone EPR5785) - LS-C138587 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5785
Western blot analysis on (A) 293T, (B) SH-SY5Y, (C) HepG2, and (D) HeLa cell lysates using anti-TIMM50 antibody.

Anti-TIMM50 Antibody (C-Terminus) - LS-C170658 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-TIMM50 Antibody (aa396-409) - LS-C55595 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody staining (0.5 ug/ml) of Human Heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-TIMM50 Antibody (aa74-104) - LS-C157198 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
TIMM50 Antibody western blot of MDA-MB453 cell line lysates (35 ug/lane). The TIMM50 antibody detected the TIMM50 protein (arrow).

Anti-TIMM50 Antibody (Internal, clone EPR5784) - LS-C138516 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5784
IHC of paraffin-embedded liver tissue using anti-TIMM50 antibody.

Anti-TIMM50 Antibody (aa25-56) - LS-C344325 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysates from HeLa, HepG2, SH-SY5Y cell line (from left to right) with TIMM50 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

TIMM50 Antibody - NBP2-38791 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: TIMM50 Antibody [NBP2-38791] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG

TIMM50 Antibody - NB100-1462 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: TIMM50 Antibody [NB100-1462] - NB100-1462 staining (0.5ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

TIMM50 Antibody (EPR5785) - NBP1-95783 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5785
Western Blot: TIMM50 Antibody (EPR5785) [NBP1-95783] (A) 293T, (B) SH-SY5Y, © HepG2, and (D) HeLa cell lysates.
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