NTMT1

Gene Symbol NTMT1
Entrez Gene 28989
Alt Symbol AD-003, C9orf32, HOMT1A, METTL11A, NRMT, NTM1A
Species Human
Gene Type protein-coding
Description N-terminal Xaa-Pro-Lys N-methyltransferase 1
Other Description N-terminal RCC1 methyltransferase|X-Pro-Lys N-terminal protein methyltransferase 1A|alpha N-terminal protein methyltransferase 1A|methyltransferase-like protein 11A
Swissprots A8K8G7 Q9BV86 Q9UI28 Q5SZB9 A8K4J2
Accessions CAD34801 EAW87893 EAW87894 EAW87895 Q9BV86 AF110776 AAF14859 AI802417 AK290957 BAF83646 AK292332 BAF85021 AK298840 BAG60968 AK307056 AK313829 BC001396 AAH01396 BC033234 AAH33234 BE392289 BF316784 BG468453 BI083998 BM009113 BM554138 BQ636193 BQ923165 CN406947 HY058177 XM_005251939 XP_005251996 NM_001286796 NP_001273725 NM_001286797 NP_001273726 NM_001286798 NP_001273727 NM_001286799 NP_001273728 NM_001286800 NP_001273729 NM_001286801 NP_001273730 NM_001286802 NP_001273731 NM_001286803 NP_001273732 NM_014064 NP_054783 NR_104596
Function Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1. {ECO:0000269|PubMed:20481588, ECO:0000269|PubMed:20668449, ECO:0000269|PubMed:24090352}.
Subcellular Location Nucleus {ECO:0000269|PubMed:20668449, ECO:0000269|PubMed:24090352}. Note=Predominantly nuclear.

NRMT (D9D6P) Rabbit mAb - 13432 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9D6P
Western blot analysis of extracts from various cell lines using NRMT (D9D6P) Rabbit mAb.

METTL11A (T-13) - sc-243462 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-METTL11A antibody - ab102664 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-METTL11A antibody (ab102664) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-METTL11A antibody - ab72660 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-METTL11A antibody (ab72660) at 1/500 dilution + Human liver lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-NTMT1 / C9orf32 Antibody - LS-C190122 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NTMT1 / C9orf32 Antibody - LS-C308958 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-NTMT1 / C9orf32 Antibody - LS-C346385 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of A549 cell using NTMT1 antibody. Blue: DAPI for nuclear staining.

Anti-NTMT1 / C9orf32 Antibody (aa1-50) - LS-C289958 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse NRMT by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Ramos (R; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NRMT antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. NRMT was also immunoprecipitated by rabbit anti-NRMT antibody BL11696, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

alpha-N-terminal Methyltransferase 1A/METTL11A Antibody - AF7159 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human alpha ‑N‑terminal Methyltransferase 1A/METTL11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha ‑N‑terminal Methyltransferase 1A/METTL11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

alpha-N-terminal Methyltransferase 1A/METTL11A Antibody - NBP1-89255 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: METTL11A Antibody [NBP1-89255] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Negative control (vector only transfected HEK293T lysate)<br/>Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415489)

alpha-N-terminal Methyltransferase 1A/METTL11A Antibody - H00028989-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: METTL11A Antibody [H00028989-B01] - C9orf32 MaxPab polyclonal antibody (B01), Lot # 08080 WULz. Western Blot analysis of C9orf32 expression in human liver.

METTL11A Polyclonal Antibody - bs-6057R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with METTL11A Polyclonal Antibody (bs-6057R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Anti-NTMT1 antibody produced in rabbit - SAB4301165 from Sigma-Aldrich

Prices $230.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NTMT1 antibody produced in rabbit - HPA020092 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-METTL11A: <B>Cat. No. HPA020092</B>: Immunohistochemical staining of human adrenal gland shows cytoplasmic positivity in cortical cells.

Anti-C9orf32 antibody produced in mouse - SAB1400511 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Polyclonal Anti-C9orf32, antibody produced in mouse, purified immunoglobulin: <B>Cat. No. SAB1400511</B>: Antibody concentration: 1.0 ~ 5.0 μg/mL. Specific band of ~25.387 kDa using human liver lysate.

METTL11A antibody - GTX120310 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-METTL11A antibody used in ICC/IF. GTX120310

Human alpha -N-terminal Methyltransferase 1A/ METTL11A Antibody - AF7159 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Detection of Human a-N-terminal Methyltransferase 1A/ METTL11A by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human a-N-terminal Methyltransferase 1A/METTL11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for a-N-terminal Methyltransferase 1A/ METTL11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .

Human METTL11A Affinity Purified Polyclonal Ab, Sheep IgG - AF7159-SP from R&D Systems

Host Sheep
Clonality Polyclonal
Detection of Human alpha -N-terminal Methyltransferase 1A/METTL11A by Western Blot.Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human alpha ‑N‑terminal Methyltransferase 1A/METTL11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7159) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha ‑N‑terminal Methyltransferase 1A/METTL11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

NTMT1 Polyclonal Antibody - A7098 from ABclonal

Prices $130.00, $220.00, $380.00
Sizes 50 µl, 100 µl, 200 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using NTMT1 antibody.

METTL11A antibody - 70R-18485 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

N-terminal Xaa-Pro-Lys N-methyltransferase 1 Antibody - abx109338 from Abbexa

Host Rabbit
Clonality Polyclonal

NTMT1 Antibody - abx005364 from Abbexa

Host Rabbit
Clonality Polyclonal
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