| Gene Symbol | ASTL |
|---|---|
| Entrez Gene | 431705 |
| Alt Symbol | SAS1B |
| Species | Human |
| Gene Type | protein-coding |
| Description | astacin-like metallo-endopeptidase (M12 family) |
| Other Description | astacin-like metalloendopeptidase|astacin-like metalloendopeptidase (M12 family)|oocyte astacin|ovastacin|sperm acrosomal SLLP1 binding |
| Swissprots | Q3KNT0 Q6HA08 |
| Accessions | EAW71389 Q6HA08 AJ537600 CAD61265 BC107126 AAI07127 BC107127 AAI07128 XM_011511205 XP_011509507 XM_011511206 XP_011509508 XM_011511207 XP_011509509 XM_011511208 XP_011509510 NM_001002036 NP_001002036 |
| Function | Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding (By similarity). {ECO:0000250}. |
| Subcellular Location | Cytoplasm {ECO:0000250}. Cell membrane {ECO:0000250}. Cytoplasmic granule {ECO:0000250}. Cytoplasmic vesicle, secretory vesicle {ECO:0000250}. Note=Secretory granules. Localizes to the peripheral cortical granules of ovulated eggs. Probably exocytosed from cortical granules during post- fertilization. Detected throughout the ooplasm of germinal vesicle stage oocytes in early bilaminar secondary follicles shortly after birth. Detected in the microvillar domain of the oolemma in arrested ovulated secondary oocytes and in the first polar body prior to fertilization. Upon fertilization, detected in the perivitelline space (PVS). Colocalizes with SPACA3 at the microvillar domain of the oolemma and in the perivitelline space (PVS) (By similarity). {ECO:0000250}. |
| Tissue Specificity | Expressed in promyelocytic leukemia HL-60 cells, Burkitt's lymphoma Raji cells, and also in some ovarian carcinomas. Not detected in normal tissues. {ECO:0000269|PubMed:15087446}. |
ASTL CRISPR/Cas9 KO Plasmid (h) - sc-416017 from Santa Cruz Biotechnology
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ASTL HDR Plasmid (h) - sc-416017-HDR from Santa Cruz Biotechnology
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ASTL Double Nickase Plasmid (h) - sc-416017-NIC from Santa Cruz Biotechnology
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ASTL Double Nickase Plasmid (h2) - sc-416017-NIC-2 from Santa Cruz Biotechnology
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ASTL CRISPR Activation Plasmid (h) - sc-416017-ACT from Santa Cruz Biotechnology
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ASTL CRISPR Activation Plasmid (h2) - sc-416017-ACT-2 from Santa Cruz Biotechnology
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ASTL Lentiviral Activation Particles (h) - sc-416017-LAC from Santa Cruz Biotechnology
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ASTL Lentiviral Activation Particles (h2) - sc-416017-LAC-2 from Santa Cruz Biotechnology
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ASTL siRNA (h) - sc-94887 from Santa Cruz Biotechnology
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ASTL shRNA Plasmid (h) - sc-94887-SH from Santa Cruz Biotechnology
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ASTL shRNA (h) Lentiviral Particles - sc-94887-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU016041 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3616 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3616 from Sigma-Aldrich
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ASTL siRNA (Human) - CRJ8220 from Cohesion Biosciences
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ASTL - MBS8227647 from MyBioSource
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shRNA set against Human Astl (NM_001002036.3) - SHH241718 from Creative biogene
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shRNA set against Human ASTL(NM_001002036.3) - SHG078447 from Creative biogene
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Human ASTL siRNA - orb278721 from Biorbyt
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ASTL shRNA Plasmid (Human) - CHJ8220 from Cohesion Biosciences
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ASTL miRNA 3'UTR clone - MiUTR1H-00646 from Creative biogene
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