MKRN1

Gene Symbol MKRN1
Entrez Gene 23608
Alt Symbol RNF61
Species Human
Gene Type protein-coding
Description makorin ring finger protein 1
Other Description E3 ubiquitin-protein ligase makorin-1|RING finger protein 61
Swissprots Q9UEZ7 Q59G11 Q9UHC7 Q6GSF1 A4D1T7 B3KXB4 Q256Y7 Q9H0G0 Q9UHW2
Accessions AAF18979 EAL24026 EAW83949 EAW83950 EAW83951 EAW83952 EAW83953 Q9UHC7 AB209298 BAD92535 AF117233 AAF17214 AF192784 AAF17487 AI828363 AK024987 AK027137 AK127030 BAG54426 AK297361 BAG59808 AK315552 BAG37929 AL136812 CAB66746 AM236048 CAJ84705 BC012398 BC025955 AAH25955 BC037400 AAH37400 BC051821 BC064838 AAH64838 BC127216 AAI27217 CA438497 DC400746 DQ892404 ABM83330 EU176516 ABW03317 XM_011515996 XP_011514298 XM_011515997 XP_011514299 XM_011515998 XP_011514300 NM_001145125 NP_001138597 NM_001291663 NP_001278592 NM_013446 NP_038474 NR_117084
Function E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. {ECO:0000269|PubMed:16785614, ECO:0000269|PubMed:19536131}.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:10843807, ECO:0000269|PubMed:16785614}.

Makorin-1 (C-20) - sc-67672 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Makorin-1 (C-20): sc-67672. Western blot analysis of Makorin-1 expression in non-transfected: sc-117752 (A) and human Makorin-1 transfected: sc-117304 (B) 293T whole cell lysates.

Makorin-1 (Z-21) - sc-133771 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Makorin-1 (Z-21): sc-133771. Western blot analysis of Makorin-1 expression in non-transfected: sc-117752 (A) and human Makorin-1 transfected: sc-117304 (B) 293T whole cell lysates.

Anti-MKRN1 antibody - ab121106 from Abcam

Prices $443.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and cytoplasm. Recommended concentration of ab121106 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Anti-MKRN1 antibody - ab139313 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-MKRN1 antibody (ab139313) at 1/500 dilutionLane 1 : Jurkat total cell extractLane 2 : Mouse thymus total cell extractLane 3 : Mouse brain total cell extract

Anti-MKRN1 antibody - ab57176 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57176 MKRN1 antibody at 1ug/ml.

Anti-MKRN1 antibody [3F9] - ab119096 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F9
All lanes : Anti-MKRN1 antibody [3F9] (ab119096) at 1/500 dilutionLane 1 : Lysate from HEK293T cells transfected with vector alone.Lane 2 : Lysate from HEK293T cells transfected with a vector expressing full length MKRN1.Lysates/proteins at 5 µg per lane.developed using the ECL technique

Anti-MKRN1 Antibody (C-Terminus) - LS-C72008 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MKRN1 Antibody (N-Terminus) - LS-C144835 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MKRN1 Antibody - LS-C124027 from LifeSpan Bioscience

Prices $575.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MKRN1 Antibody (aa371-420) - LS-C31990 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Jurkat Cell Lysate

Anti-MKRN1 Antibody (aa184-213, AP) - LS-C237672 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MKRN1 Antibody (aa101-150) - LS-C31947 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
A: Marker, B: Jurkat Cell Lysate

Anti-MKRN1 Antibody (aa184-213, APC) - LS-C237673 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MKRN1 Antibody (aa184-213, FITC) - LS-C237675 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MKRN1 Antibody (aa184-213, Biotin) - LS-C237674 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MKRN1 Antibody (aa184-213, PE) - LS-C237677 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MKRN1 Antibody (aa184-213, Azide-free, HRP) - LS-C248825 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MKRN1 Antibody (clone 1G6) - LS-C173276 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G6
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKRN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKRN1.

Anti-MKRN1 Antibody (aa365-441) - LS-C309009 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-MKRN1 Antibody (aa184-213) - LS-C323018 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MKRN1 Antibody (aa105-118) - LS-C154849 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
MKRN1 antibody HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with (0.3 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Anti-MKRN1 Antibody (aa184-213) - LS-C157888 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
MKRN1 Antibody western blot of A549 cell line lysates (35 ug/lane). The MKRN1 antibody detected the MKRN1 protein (arrow).

Anti-MKRN1 Antibody (C-Terminus) - LS-C286037 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human MKRN1 by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MKRN1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-MKRN1 Antibody (clone 3F9) - LS-C337479 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKRN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MKRN1.

Anti-MKRN1 Antibody (C-Terminus) - LS-C286709 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human MKRN1 by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-MKRN1 used at a dilution of 1:500. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.
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