RASGRP4

Gene Symbol RASGRP4
Entrez Gene 115727
Alt Symbol -
Species Human
Gene Type protein-coding
Description RAS guanyl releasing protein 4
Other Description RAS guanyl-releasing protein 4|guanyl nucleotide releasing protein 4
Swissprots Q96QN7 Q96QN5 C9J416 C0LTP3 C0LTP7 Q8N858 C0LTP4 Q96QN6 A6H8M4 C0LTP2 C9JHZ1 Q8TDF6 C0LTP5
Accessions EAW56794 Q8TDF6 AF448437 AAL87858 AK097292 AY048119 AAK85701 AY048120 AAK85702 AY048121 AAK85703 BC142721 BC146669 AAI46670 BC150202 AAI50203 BC151216 BQ028385 FJ768677 ACN82414 FJ768678 ACN82415 FJ768679 ACN82416 FJ768680 ACN82417 FJ768681 ACN82418 FJ768682 ACN82419 XR_935732 NM_001146202 NP_001139674 NM_001146203 NP_001139675 NM_001146204 NP_001139676 NM_001146205 NP_001139677 NM_001146206 NP_001139678 NM_001146207 NP_001139679 NM_170604 NP_733749 NM_052949 NM_170602 NM_170603
Function Functions as a cation- and diacylglycerol (DAG)- regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation. {ECO:0000269|PubMed:11880369, ECO:0000269|PubMed:11956218, ECO:0000269|PubMed:12493770, ECO:0000269|PubMed:18024961}.
Subcellular Location Cytoplasm. Cell membrane. Note=Recruited to membranes upon activation by DAG.
Tissue Specificity Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient. {ECO:0000269|PubMed:11880369, ECO:0000269|PubMed:11956218}.
Top Pathways MAPK signaling pathway

RasGRP4 (H-59) - sc-292930 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RasGRP4 (H-59): sc-292930. Western blot analysis of RasGRP4 expression in HEL 92.1.7 (A), Raji (B), Jurkat (C), THP-1 (D), AML-193 (E) and J774.A1 (F) whole cell lysates.

RasGRP4 (B-16) - sc-81900 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
RasGRP4 (B-16): sc-81900. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil tissue showing membrane and cytoplasmic localization.

RasGRP4 (E-13) - sc-131336 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RasGRP4 (E-13): sc-131336. Western blot analysis of RasGRP4 expression in mouse thymus tissue extract (A) and human PBL whole cell lysate (B).

Anti-RASGRP 4 antibody - ab96293 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab96293 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96293 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-RASGRP 4 antibody - ab57978 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
RASGRP 4 antibody (ab57978) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.

Anti-RASGRP 4 antibody - ab106188 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RASGRP 4 antibody - ab118735 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RASGRP 4 antibody (ab118735) at 1 µg/ml + Jurkat cell lysate at 10 µg

Anti-RASGRP4 Antibody (Internal) - LS-C145016 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RASGRP4 Antibody (aa574-674, Azide-free) - LS-C328479 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-RASGRP4 Antibody (aa149-198) - LS-C145724 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RASGRP4 antibody LS-C145724 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.

Anti-RASGRP4 Antibody (clone 6D3) - LS-C133832 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D3
Immunoperoxidase of monoclonal antibody to RASGRP4 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]

Anti-RASGRP4 Antibody (clone 1F3) - LS-C133831 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1F3
Immunoperoxidase of monoclonal antibody to RASGRP4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Anti-RASGRP4 Antibody (aa108-200) - LS-C185646 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded SW480 xenograft using RASGRP4 antibody at 1:100 dilution.

RASGRP 4 Antibody - NBP1-92317 from Novus Biologicals

Host Rabbit
Clonality Polyclonal
Western Blot: RASGRP 4 Antibody [NBP1-92317] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Human cell line RT-4

RASGRP 4 Antibody (1F3) - H00115727-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1F3

RASGRP 4 Antibody (6D3) - H00115727-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D3
Immunohistochemistry-Paraffin: RASGRP 4 Antibody (6D3) [H00115727-M02] - Analysis of monoclonal antibody to RASGRP4 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.

RASGRP 4 Antibody - H00115727-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot analysis of RASGRP4 expression in transfected 293T cell line by RASGRP4 MaxPab polyclonal antibody (B01). Lane1: RASGRP4 transfected lysate (74.90 KDa). Lane2: Non-transfected lysate.

RASGRP 4 Antibody - H00115727-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: RASGRP 4 Antibody [H00115727-D01P] - Analysis of RASGRP4 expression in transfected 293T cell line by RASGRP4 polyclonal antibody.Lane 1: RASGRP4 transfected lysate(74.90 KDa).Lane 2: Non-transfected lysate.

Monoclonal Anti-RASGRP4, (C-terminal) antibody produced in mouse - SAB1405343 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D3
<B>Immunohistochemistry</B><BR/>Monoclonal Anti-RASGRP4: <B>Cat. No. SAB1405343</B>: Antibody concentration: 3 μg/mL using human kidney tissue.

Monoclonal Anti-RASGRP4 antibody produced in mouse - WH0115727M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1F3
<B>Immunoblotting</B><BR/>Monoclonal Anti-RASGRP4, <B>Cat. No. WH0115727M1</B> used at the antibody concentration: 1 μg/mL. Specific band of ~37.11 kDa using immunogen protein lysate.

Anti-RASGRP4 antibody produced in rabbit - SAB1410200 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RASGRP4: <B>Cat. No. SAB1410200</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~74.9 kDa using RASGRP4 transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

RASGRP4 antibody - N-terminal region (ARP62124_P050) - ARP62124_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RASGRP4-Antibody-monoclonal-M02 - AT3581a from Abgent, a WuXi AppTec company

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6D3
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .

RASGRP4-Antibody-monoclonal-M01 - AT3580a from Abgent, a WuXi AppTec company

Prices $315.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1F3
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .

RASGRP4 antibody - orb69878 from Biorbyt

Prices $723.00
Sizes 100 μl
Host Rabbit
Clonality Polyclonal
Western Blot analysis of A:Jurkat whole cell lysate using RASGRP4 antibody
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