OSGEP

Gene Symbol OSGEP
Entrez Gene 55644
Alt Symbol GCPL1, KAE1, OSGEP1, PRSMG1
Species Human
Gene Type protein-coding
Description O-sialoglycoprotein endopeptidase
Other Description N6-L-threonylcarbamoyladenine synthase|hOSGEP|probable O-sialoglycoprotein endopeptidase|probable tRNA N6-adenosine threonylcarbamoyltransferase|probable tRNA threonylcarbamoyladenosine biosynthesis protein OSGEP|t(6)A synthase|t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEP|tRNA threonylcarbamoyladenosine biosynthesis protein OSGEP
Swissprots Q6IAC3 Q9NPF4
Accessions BAB33147 EAW66469 EAW66470 Q9NPF4 AB050442 BAB33172 AF052137 AJ271669 CAB71031 AK000418 BAA91150 AK054823 AK055492 BC032310 AAH32310 BX428958 CD365329 CR457232 CAG33513 DA068526 DQ894806 ABM85732 EU176277 ABW03728 XM_011536930 XP_011535232 XM_011536931 XP_011535233 XM_011536932 XP_011535234 NM_017807 NP_060277
Function Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. {ECO:0000255|HAMAP-Rule:MF_03180}.
Subcellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03180}. Nucleus {ECO:0000255|HAMAP-Rule:MF_03180, ECO:0000269|PubMed:22912744}.
Tissue Specificity Widely expressed at low level. Expressed in heart, placenta, liver, kidney, lung, brain, skeletal muscle and pancreas. {ECO:0000269|PubMed:12039036}.

Osgep (H-3) - sc-393199 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Osgep (H-3): sc-393199. Western blot analysis of Osgep expression in Ramos (A), HeLa (B), SK-BR-3 (C), JAR (D) and PC-3 (E) whole cell lysates and mouse lymph node tissue extract (F).

Osgep (Y-14) - sc-160634 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Osgep (Y-14): sc-160634. Western blot analysis of Osgep expression in Ramos (A), HeLa (B) and JAR (C) whole cell lysates and mouse brain tissue extract (D).

Osgep (H-247) - sc-366074 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Osgep (H-247): sc-366074. Western blot analysis of Osgep expression in Ramos (A), JAR (B), HeLa (C), PC-3 (D), SK-BR-3 (E) and PC-12 (F) whole cell lysates.

Osgep (R-15) - sc-160633 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Osgep (R-15): sc-160633. Western blot analysis of Osgep expression in HeLa (A) and SK-BR-3 (B) whole cell lysates and mouse brain tissue extract (C).

Osgep (N-17) - sc-160632 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-OSGEP antibody - ab96192 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-OSGEP antibody (ab96192) at 1/2000 dilution + Raji whole cell lysate at 30 µg

Anti-OSGEP antibody - ab101656 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-OSGEP antibody (ab101656) at 1/500 dilution + Human fetal liver lysate

Anti-OSGEP antibody - ab68742 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-OSGEP antibody (ab68742) at 1/500 dilutionLane 1 : OSGEP transfected 293T cell lysateLane 2 : non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-OSGEP antibody - ab98281 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-OSGEP antibody (ab98281) at 1 µg/ml + MCF7 cell lysate at 10 µg

Anti-OSGEP antibody [8B3] - ab119067 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8B3
All lanes : Anti-OSGEP antibody [8B3] (ab119067) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY OSGEP cDNALysates/proteins at 5 µg per lane.

Anti-OSGEP antibody [9E3] - ab119311 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9E3
All lanes : Anti-OSGEP antibody [9E3] (ab119311) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY OSGEP cDNALysates/proteins at 5 µg per lane.

Anti-OSGEP Antibody (clone 9E3) - LS-C173008 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9E3
IHC of paraffin-embedded Human Kidney tissue using anti-OSGEP mouse monoclonal antibody.

Anti-OSGEP Antibody (clone 8B3) - LS-C173007 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8B3
IHC of paraffin-embedded Human Kidney tissue using anti-OSGEP mouse monoclonal antibody.

Anti-OSGEP Antibody (aa143-192) - LS-C107985 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
OSGEP antibody LS-C107985 Western blot of 721_B cell lysate.

Anti-OSGEP Antibody (aa35-84) - LS-C107984 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
OSGEP antibody LS-C107984 Western blot of MCF7 cell lysate.

Anti-OSGEP Antibody (aa140-335) - LS-C185740 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded Colon ca using OSGEP antibody at 1:500 dilution.

OSGEP Antibody (9E3) - NBP2-00823 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 9E3
Western Blot: OSGEP Antibody (9E3) [NBP2-00823] Analysis of extracts (35ug) from 9 different cell lines by using anti-OSGEP monoclonal antibody.

OSGEP Antibody - NBP1-83378 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: OSGEP Antibody [NBP1-83378] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and subset of cells in renal glomeruli.

OSGEP Antibody - H00055644-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: OSGEP Antibody [H00055644-B01P] - Analysis of OSGEP expression in transfected 293T cell line by OSGEP polyclonal antibody. Lane 1: OSGEP transfected lysate(36.85 KDa). Lane 2: Non-transfected lysate.

Anti-OSGEP (301-315) antibody produced in rabbit - SAB1101882 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human OSGEP was separated on SDS-PAGE, blotted with Anti-OSGEP (301-315) (<B>Cat. No. SAB1101882</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + OSGEP immunizing peptide (human, 301-315)

Anti-OSGEP, (N-terminal) antibody produced in rabbit - SAB2105186 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-OSGEP, antibody produced in rabbit: <B>Cat. No. SAB2105186</B>: Immunoblotting analysis of OSGEP in MCF7 cell lysate with OSGEP anitbody at 1.0μg/mL.

Anti-OSGEP (316-330) antibody produced in rabbit - SAB1101883 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human OSGEP was separated on SDS-PAGE, blotted with Anti-OSGEP (316-330) (<B>Cat. No. SAB1101883</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + OSGEP immunizing peptide (human, 316-330)

Anti-OSGEP antibody produced in rabbit - HPA039751 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-OSGEP: <B>Cat. No. HPA039751</B>: Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules and subset of cells in renal glomeruli.

Anti-OSGEP antibody produced in rabbit - SAB2105187 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-OSGEP, antibody produced in rabbit: <B>Cat. No. SAB2105187</B>: Immunoblotting analysis of OSGEP in 721_B cell lysate with OSGEP anitbody at 1.0μg/mL.

Anti-OSGEP antibody produced in mouse - SAB1407680 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-OSGEP: <B>Cat. No. SAB1407680</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~36.9 kDa using OSGEP transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.
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