GFI1

Gene Symbol GFI1
Entrez Gene 2672
Alt Symbol GFI-1, GFI1A, SCN2, ZNF163
Species Human
Gene Type protein-coding
Description growth factor independent 1 transcription repressor
Other Description growth factor independence-1|growth factor independent protein 1|zinc finger protein 163|zinc finger protein Gfi-1
Swissprots Q99684 Q8N564
Accessions CDL93503 EAW73092 EAW73093 EAW73094 Q99684 AB209606 BAD92843 AK123009 BAG53846 AK313876 BC032751 AAH32751 BC074866 AAH74866 BC074867 AAH74867 BE242676 BM150826 BQ336135 DA927584 DB129173 DB137690 DQ891273 ABM82199 DQ894461 ABM85387 U67369 AAB37272 XM_005270749 XP_005270806 XM_011541245 XP_011539547 XM_011541246 XP_011539548 NM_001127215 NP_001120687 NM_001127216 NP_001120688 NM_005263 NP_005254
Function Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2- GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin- mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1- dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB p
Subcellular Location Nucleus {ECO:0000269|PubMed:11060035, ECO:0000269|PubMed:12874834, ECO:0000269|PubMed:18805794, ECO:0000269|PubMed:20547752, ECO:0000269|PubMed:8754800}. Note=Colocalizes with PIAS3 and RUNX1T1 in nuclear dots.

Gfi-1 (B-9) - sc-376949 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gfi-1 (B-9): sc-376949. Western blot analysis of Gfi-1 expression in THP-1 whole cell lysate.

Gfi-1 (M-19) - sc-6357 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Gfi-1 (M-19): sc-6357. Western blot analysis of Gfi-1 expression in non-transfected: sc-117752 (A) and human Gfi-1 transfected: sc-115318 (B) 293T whole cell lysates.

Gfi-1 (G-11) - sc-373960 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Gfi-1 (G-11): sc-373960. Western blot analysis of Gfi-1 expression in THP-1 whole cell lysate.

Gfi-1 (H-200) - sc-22796 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Gfi-1 (N-20) - sc-8558 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Gfi-1 (N-20): sc-8558. Western blot analysis of Gfi-1 expression in THP-1 whole cell lysate.

Gfi-1 (15-8) - sc-101053 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Gfi-1 (15-8): sc-101053. Western blot analysis of Gfi-1 expression in non-transfected: sc-117752 (A) and human Gfi-1 transfected: sc-115318 (B) 293T whole cell lysates.

Anti-GFI1 antibody - ab21061 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GFI1 antibody (ab21061) at 1 µg/mlLane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913) with Human GFI1 peptide (ab22461) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) Performed under reducing conditions.

Anti-GFI1 antibody - ab55108 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
GFI1 antibody (ab55108) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymph node.

Anti-GFI1 antibody - ab134284 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labelling GFI1 with ab134284 at 5&microg/ml. Primary incubation was followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-GFI1 antibody - ab107589 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GFI1 antibody (ab107589) at 6 µg/mlLane 1 : Human HepG2 cell lysateLane 2 : Human HepG2 cell lysate with Immunizing peptideLane 3 : Mouse RAW cell lysateSecondaryGoat anti-rabbit HRPdeveloped using the ECL technique

Anti-GFI1 antibody [5D7] - ab105918 from Abcam

Host Mouse
Clonality Monoclonal
Clone 5D7
All lanes : Anti-GFI1 antibody [5D7] (ab105918) at 1/500 dilutionLane 1 : HEK293 cell lysate.Lane 2 : GFI1-hIgGFc transfected HEK293 cell lysate.

Anti-GFI1 Antibody (Internal) - LS-C210001 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-GFI1 Antibody (C-Terminus) - LS-C209873 from LifeSpan Bioscience

Prices $405.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-GFI1 Antibody (aa32-81) - LS-C29853 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal Lung Lysate

Anti-GFI1 Antibody (N-Terminus) - LS-C30133 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
A: Marker, B: HepG2 Cell Lysate

Anti-GFI1 Antibody (aa1-92, Azide-free) - LS-C327435 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-GFI1 Antibody (aa1-349) - LS-C311570 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-GFI1 Antibody (clone 6C10) - LS-C340198 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6C10
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFI1.

Anti-GFI1 Antibody (N-Terminus) - LS-C345643 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of GFI1 Antibody with human Jurkat Whole Cell lysate.

Anti-GFI1 Antibody (aa1-180, clone 4E2) - LS-C339814 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4E2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFI1.

Anti-GFI1 Antibody (clone 5D7) - LS-C169248 from LifeSpan Bioscience

Prices $320.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5D7
Western blot using GFI1 monoclonal antibody against HEK293 (1) and GFI1(AA: 2-250)-hIgGFc transfected HEK293 (2) cell lysate.

Anti-GFI1 Antibody (aa1-180, clone 6H7) - LS-C339633 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6H7
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFI1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFI1.

Anti-GFI1 Antibody (aa1-180, clone 6E8) - LS-C339630 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6E8
IHC of paraffin-embedded Human Kidney tissue using anti-GFI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-GFI1 Antibody (clone 3G8) - LS-C104956 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3G8
Immunofluorescence of monoclonal antibody to GFI1 on HeLa cell (antibody concentration 10 ug/ml).

Anti-GFI1 Antibody (aa280-330) IHC-plus™ - LS-B7250 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GFI1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7250 concentration 5 ug/ml.
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