CHST8

Gene Symbol CHST8
Entrez Gene 64377
Alt Symbol GALNAC4ST1, GalNAc4ST, PSS3
Species Human
Gene Type protein-coding
Description carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8
Other Description GALNAC-4-ST1|GalNAc-4-O-sulfotransferase 1|N-acetylgalactosamine-4-O-sulfotransferase 1|carbohydrate sulfotransferase 8|galNAc4ST-1
Swissprots Q9H2A9 Q9H3N2
Accessions Q9H2A9 AB047801 BAB19806 AF300612 AAG39444 AF305781 AAL09373 BC011380 AAH11380 BC014250 AAH14250 BC018723 AAH18723 BI917117 BX354555 DB500322 DQ891438 ABM82364 DQ894614 ABM85540 XM_011527220 XP_011525522 XM_011527221 XP_011525523 XM_011527222 XP_011525524 XM_011527223 XP_011525525 XM_011527224 XP_011525526 XM_011527225 XP_011525527 XM_011527226 XP_011525528 NM_001127895 NP_001121367 NM_001127896 NP_001121368 NM_022467 NP_071912
Function Catalyzes the transfer of sulfate to position 4 of non- reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Required for biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Only active against terminal GalNAcbeta1,GalNAcbeta. Not active toward chondroitin. {ECO:0000269|PubMed:10988300, ECO:0000269|PubMed:11445554}.
Subcellular Location Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.
Tissue Specificity Predominantly expressed in pituitary gland. In brain, it is expressed in pituitary gland, cerebellum, medulla oblongata, pons, thalamus and spinal cord. Expressed in the epidermis. Expressed at lower level in lung, spleen, adrenal gland, placenta, prostate, testis, mammary gland and trachea. {ECO:0000269|PubMed:10988300, ECO:0000269|PubMed:11001942, ECO:0000269|PubMed:11445554, ECO:0000269|PubMed:22289416}.

GalNAc4ST-1 (C-20) - sc-49012 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

GalNAc4ST-1 (H-86) - sc-368462 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GalNAc4ST-1 (H-86): sc-368462. Western blot analysis of GalNAc4ST-1 expression in Hep G2 (A), SH-SY5Y (B), HeLa (C), NTERA-2 cl.D1 (D), NCI-H929 (E) and MCF7 (F) whole cell lysates.

Anti-CHST8 antibody - ab136034 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-CHST8 antibody (ab136034) at 1/500 dilution + HT29 cell lysate at 30 µg

Anti-CHST8 antibody - ab171322 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-CHST8 antibody (ab171322) at 1/100 dilution + HepG2 cell line lysates at 35 µg

Anti-CHST8 antibody - ab69064 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-CHST8 antibody (ab69064) at 1/500 dilutionLane 1 : CHST8 transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-CHST8 antibody - ab104923 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-CHST8 antibody (ab104923) at 0.25 µg/ml + HepG2 cell lysate at 10 µg

Anti-GALNAC4ST1 / CHST8 Antibody (aa219-247) - LS-C168766 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
CHST8 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The CHST8 antibody detected the CHST8 protein (arrow).

Anti-GALNAC4ST1 / CHST8 Antibody (aa341-390) - LS-C119806 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from K562 cells, using CHST8 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-GALNAC4ST1 / CHST8 Antibody (aa219-247, APC) - LS-C233837 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GALNAC4ST1 / CHST8 Antibody (aa219-247, FITC) - LS-C233838 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GALNAC4ST1 / CHST8 Antibody (aa219-247, Biotin) - LS-C233839 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GALNAC4ST1 / CHST8 Antibody (aa219-247, PE) - LS-C233840 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GALNAC4ST1 / CHST8 Antibody (aa219-247, AP) - LS-C249045 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GALNAC4ST1 / CHST8 Antibody (aa219-247, Azide-free, HRP) - LS-C249674 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GALNAC4ST1 / CHST8 Antibody (aa333-382) - LS-C291801 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GALNAC4ST1 / CHST8 Antibody (aa226-255) - LS-C320387 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-GALNAC4ST1 / CHST8 Antibody (aa201-250) - LS-C81440 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HepG2 cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%.

CHST8 Antibody - NBP1-83396 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: CHST8 Antibody [NBP1-83396] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli and endoplasmic reticulum.

CHST8 Antibody - NBP1-69273 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CHST8 Antibody [NBP1-69273] - Human Fetal Liver, Antibody Dilution: 1.0 ug/ml.

CHST8 Antibody - H00064377-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: CHST8 Antibody [H00064377-B01] - analysis of CHST8 expression in transfected 293T cell line by CHST8 MaxPab polyclonal antibody (B01). Lane 1: CHST8 transfected lysate (48.80 KDa). Lane 2: Non-transfected lysate.

Anti-CHST8 antibody produced in rabbit - HPA016004 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CHST8: <B>Cat. No. HPA016004</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate shows membranous and cytoplasmic positivity in glandular cells.

Anti-CHST8 antibody produced in rabbit - AV49723 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: HepG2 (<B>Cat. No. AV49723</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 0.25 μg/mL in cell type HepG2

Anti-CHST8, C-Terminal antibody produced in rabbit - SAB4502754 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CHST8, C-Terminal: <B>Cat. No. SAB4502754</B>: Immunoblotting analysis of extracts from K562 cells, using Anti-CHST8, C-Terminal antibody. The lane on the left was treated with the Anti-CHST8, C-Terminal antibody. The lane on the right (negative control) was treated with both Anti-CHST8, C-Terminal antibody and the synthesized immunogen peptide.

CHST8 antibody - middle region (ARP49723_P050) - ARP49723_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CHST8-Antibody-Center - AP12842c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
CHST8 Antibody (Center) (Cat. #AP12842c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CHST8 antibody detected the CHST8 protein (arrow).
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