Gene Symbol | KLF1 |
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Entrez Gene | 10661 |
Alt Symbol | CDAN4, EKLF, HBFQTL6, INLU |
Species | Human |
Gene Type | protein-coding |
Description | Kruppel-like factor 1 (erythroid) |
Other Description | Krueppel-like factor 1|erythroid Kruppel-like factor|erythroid krueppel-like transcription factor|erythroid-specific transcription factor EKLF|monoclonal antibody A3D8 |
Swissprots | Q13351 Q6PIJ5 Q92899 |
Accessions | AAB51173 AAC50562 ACJ26762 ACJ26763 ACM18112 ACM18113 ACM18114 ACM18115 ACM18116 AFW90650 AHA61453 AHA61454 EAW84323 Q13351 BC033580 AAH33580 BC040000 U65404 AAC51108 XM_011527642 XP_011525944 NM_006563 NP_006554 |
Function | Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation (By similarity). {ECO:0000250}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:21055716}. Note=Colocalizes with SUMO1 in nuclear speckles. {ECO:0000250}. |
Tissue Specificity | Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines. {ECO:0000269|PubMed:8924208, ECO:0000269|PubMed:9119377}. |
EKLF CRISPR/Cas9 KO Plasmid (h) - sc-402366 from Santa Cruz Biotechnology
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EKLF HDR Plasmid (h) - sc-402366-HDR from Santa Cruz Biotechnology
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EKLF Double Nickase Plasmid (h) - sc-402366-NIC from Santa Cruz Biotechnology
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EKLF Double Nickase Plasmid (h2) - sc-402366-NIC-2 from Santa Cruz Biotechnology
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EKLF CRISPR Activation Plasmid (h) - sc-402366-ACT from Santa Cruz Biotechnology
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EKLF CRISPR Activation Plasmid (h2) - sc-402366-ACT-2 from Santa Cruz Biotechnology
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EKLF Lentiviral Activation Particles (h) - sc-402366-LAC from Santa Cruz Biotechnology
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EKLF Lentiviral Activation Particles (h2) - sc-402366-LAC-2 from Santa Cruz Biotechnology
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EKLF siRNA (h) - sc-37831 from Santa Cruz Biotechnology
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EKLF shRNA Plasmid (h) - sc-37831-SH from Santa Cruz Biotechnology
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EKLF shRNA (h) Lentiviral Particles - sc-37831-V from Santa Cruz Biotechnology
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KLF1 RNAi - H00010661-R01 from Novus Biologicals
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MISSION® esiRNA - EHU018151 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN11243 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND11243 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR05415 from Sigma-Aldrich
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KLF1 siRNA (Human) - CRH7269 from Cohesion Biosciences
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EKLF CRISPR/Cas9 KO Plasmid (h2) - sc-402366-KO-2 from Santa Cruz Biotechnology
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EKLF HDR Plasmid (h2) - sc-402366-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human KLF1 (NM_006563.3) - SHH325703 from Creative biogene
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KLF1 shRNA Plasmid (Human) - CHH7269 from Cohesion Biosciences
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KLF1 miRNA 3'UTR clone - MiUTR1H-05353 from Creative biogene
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