TPX2

Gene Symbol TPX2
Entrez Gene 22974
Alt Symbol C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100
Species Human
Gene Type protein-coding
Description TPX2, microtubule-associated
Other Description TPX2, microtubule-associated protein homolog|TPX2, microtubule-associated, homolog|differentially expressed in cancerous and non-cancerous lung cells 2|differentially expressed in lung cells|hepatocellular carcinoma-associated antigen 519|hepatocellular carcinoma-associated antigen 90|preferentially expressed in colorectal cancer|protein fls353|restricted expression proliferation associated protein 100|restricted expression proliferation-associated protein 100|targeting protein for Xklp2
Swissprots Q9H1R4 Q9ULW0 Q9UL00 Q9NRA3 Q9Y2M1 Q9UFN9 Q96RR5
Accessions EAW76418 EAW76419 EAW76420 EAW76421 EAW76422 Q9ULW0 AB024704 BAA76931 AB027467 BAA85893 AF098158 AAF03248 AF146731 AAD33965 AF244547 AAF81695 AF287265 AAK83033 AK001391 BAG50902 AK093379 AK312549 BAG35446 AL117534 CAB55982 AY734680 AAU29203 BC004136 AAH04136 BC011357 AAH11357 BC020207 AAH20207 BM835456 BU181208 CB960160 JF432351 ADZ15568 XM_011528697 XP_011526999 XM_011528698 XP_011527000 XM_011528699 XP_011527001 XM_011528700 XP_011527002 NM_012112 NP_036244
Function Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. {ECO:0000269|PubMed:18663142, ECO:0000269|PubMed:19208764}.
Subcellular Location Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
Tissue Specificity Expressed in lung carcinoma cell lines but not in normal lung tissues.

TPX2 (D2R5C) XP ® Rabbit mAb - 12245 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2R5C
Western blot analysis of extracts from various cell lines using TPX2 (D2R5C) XP ® Rabbit mAb.

TPX2 (D9Y1V) Rabbit mAb - 12833 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9Y1V
Western blot analysis of extracts from various cell lines using TPX2 (D9Y1V) Rabbit mAb.

TPX2 Antibody - 8559 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using TPX2 Antibody.

TPX2 (B-5) - sc-376812 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TPX2 (B-5): sc-376812. Western blot analysis of TPX2 expression in K-562 whole cell lysate.

TPX2 (E-2) - sc-271570 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
TPX2 (E-2): sc-271570. Immunofluorescence staining of formalin-fixed HeLa cells showing cytoplasmic localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

TPX2 (C-21) - sc-26275 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TPX2 (C-21): sc-26275. Western blot analysis of TPX2 expression in K-562 whole cell lysate.

TPX2 (F-17) - sc-26274 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TPX2 (F-17): sc-26274. Western blot analysis of human recombinant TPX2 fusion protein (A) and HeLa (B), K-562 (C), SHP-77 (D), A549 (E) and NIH/3T3 (F) whole cell lysates.

TPX2 (H-300) - sc-32863 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
TPX2 (H-300): sc-32863. Western blot analysis of TPX2 expression in K-562 (A) and Jurkat (B) whole cell lysates.

TPX2 (K-14) - sc-26277 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TPX2 (K-14): sc-26277. Western blot analysis of TPX2 expression in NIH/3T3 nuclear extract.

TPX2 (P-17) - sc-26276 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TPX2 (P-17): sc-26276. Western blot analysis of TPX2 expression in SHP-77 whole cell lysate.

TPX2 (N-16) - sc-26273 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
TPX2 (N-16): sc-26273. Western blot analysis of TPX2 expression in K-562 whole cell lysate.

TPX2 (18D5) - sc-53775 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
TPX2 (18D5): sc-53775. Western blot analysis of TPX2 expression in HeLa (A), K-562 (B) and Jurkat (C) whole cell lysates.

Anti-TPX2 antibody - ab71816 from Abcam

Prices $379.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-TPX2 antibody (ab71816) at 1/100 dilution + human placenta lysate at 12.5 µg

Anti-TPX2 antibody - ab70237 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-TPX2 antibody (ab70237) at 0.1 µg/mlLane 1 : HeLa cell lysate at 5 µgLane 2 : HeLa cell lysate at 15 µgLane 3 : HeLa cell lysate at 50 µgdeveloped using the ECL technique

Anti-TPX2 antibody - ab126976 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-TPX2 antibody (ab126976) at 1/1000 dilution + HeLa whole cell lysate at 30 µgPerformed under reducing conditions.

Anti-TPX2 antibody - ab87324 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab87324, at 1/250 dilution, staining TPX2 in formalin-fixed, paraffin-embedded Human lung adenocarcinoma by Immunohistochemistry using DAB staining.

Anti-TPX2 antibody [18D5] - ab115628 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 18D5
ab115628, at 10 µg/ml, staining TPX2 in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry. Following primary incubation, slides were incubated with biotinylated goat anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-TPX2 antibody [18D5-1] - ab32795 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 18D5-1
ab32795 staining TPX2 in HeLa cells and mouse NIH-3T3 cells (fuzzier pattern, different from the high-quality sharp signal seen in Human cells), by immunofluorescence. optimal antibody dilution: 4µg/mloptimal fixation protocol: PFA/Triton fixation: 10 min room at room temperature, in 3,7 % PFA diluted in PHEM buffer (45  mM Hepes pH 6,9, 45 mM Pipes pH 6,9, 5 mM MgCl2, 10 mM EGTA) containing 0.2% Triton X-100, followed  by 3 washes in PBS    -  Alternative fixation protocol also gives good staining: 6 min in cold Methanol at -20°C,  then 3 washes in PBS. IF was performed following a standard protocol: Blocking, 30 min; primary antibody, 1 hr; secondary antibody, 45 min. All incubations were at 37 °C in PBS/ 0.1% Tween containing 3% BSA.

Anti-TPX2 Antibody - LS-C48678 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-TPX2 Antibody - LS-C76463 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-TPX2 Antibody (C-Terminus) - LS-C90644 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-TPX2 Antibody (aa150-200) - LS-C90645 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TPX2 Antibody (C-Terminus) - LS-C90646 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TPX2 Antibody - LS-C132596 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-TPX2 Antibody (aa1-220, clone 1D4B) - LS-C189761 from LifeSpan Bioscience

Prices $335.00
Sizes 25 µg
Host Mouse
Clonality Monoclonal
Clone 1D4B
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