WDYHV1

Gene Symbol WDYHV1
Entrez Gene 55093
Alt Symbol C8orf32
Species Human
Gene Type protein-coding
Description WDYHV motif containing 1
Other Description N-terminal Gln amidase|WDYHV motif-containing protein 1|nt(Q)-amidase|protein N-terminal glutamine amidohydrolase|protein NH2-terminal glutamine deamidase
Swissprots B4DE68 Q96HA8 Q9NW95
Accessions EAW92037 EAW92038 EAW92039 Q96HA8 AK001066 BAA91488 AK225643 AK293492 BAG56979 AU119047 BC008781 AAH08781 BG528966 HQ448315 ADQ32795 XM_006716597 XP_006716660 XM_011517146 XP_011515448 XM_011517147 XP_011515449 XM_011517148 XP_011515450 XM_011517149 XP_011515451 XM_011517150 XP_011515452 XM_011517151 XP_011515453 XR_928337 XR_928338 NM_001283024 NP_001269953 NM_001283027 NP_001269956 NM_018024 NP_060494
Function Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N- terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C- terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm, cytosol {ECO:0000250}. Nucleus {ECO:0000250}.

Anti-wdyhv1 antibody - ab79869 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-wdyhv1 antibody (ab79869) at 1/50 dilution + Mouse heart tissue lysate at 35 µg

Anti-wdyhv1 antibody - N-terminal - ab182530 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-wdyhv1 antibody - N-terminal (ab182530) at 1 µg/ml + HepG2 whole cell lysates at 10 µg

Anti-WDYHV1 Antibody (aa30-79) - LS-C201950 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human HepG2. WDYHV1 antibody dilution 1.0 ug/ml.

Anti-WDYHV1 Antibody (aa106-136) - LS-C97296 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with C8orf32 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

wdyhv1 Antibody - NBP1-86722 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: wdyhv1 Antibody [NBP1-86722] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Anti-WDYHV1 antibody produced in rabbit - HPA024823 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-WDYHV1: <B>Cat. No. HPA024823</B>: Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Anti-WDYHV1 (116-130) antibody produced in rabbit - SAB1102347 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human WDYHV1 was separated on SDS-PAGE, blotted with Anti-WDYHV1 (116-130) (<B>Cat. No. SAB1102347</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + WDYHV1 immunizing peptide (human, 116-130)

WDYHV1 Antibody - N-terminal region (ARP72013_P050) - ARP72013_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

WDYHV1 Antibody - middle region (OAAB01519) - OAAB01519 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

WDYHV1-Antibody-Center - AP2809c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of anti-WDYHV1 Antibody (Center) Pab (Cat.#AP2809c) in mouse heart tissue lysates (35ug/lane). WDYHV1(arrow) was detected using the purified Pab.

WDYHV1 antibody - orb155418 from Biorbyt

Prices $425.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal

WDYHV1 Antibody - abx036296 from Abbexa

Host Rabbit
Clonality Polyclonal

WDYHV1 (Center) antibody - AP12459PU-N from Acris Antibodies

Prices $330.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with C8orf32 antibody (Cat#AP12459PU-N) (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

WDYHV1 antibody - H00055093-B01P from Acris Antibodies

Prices $1,530.00
Sizes 500 µg
Host Mouse
Clonality Polyclonal

WDYHV1, Polyclonal Antibody - MBS8504342 from MyBioSource

Prices $330.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

WDYHV1 Antibody - E307071 from Enogene

Prices $275.00
Sizes 100ug/200ul

Rabbit Anti-Human WDYHV1 Polyclonal Antibody - DPABH-14084 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

WDYHV1 Polyclonal Antibody - PA5-12414 from Thermo Fisher Scientific

Prices $349.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue using a WDYHV1 polyclonal antibody (Product ## PA5-12414), followed by HRP-conjugated secondary antibody and DAB staining.

WDYHV1, Polyclonal Antibody - MBS9205634 from MyBioSource

Prices $155.00, $340.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal

Anti-WDYHV1 (center) antibody produced in rabbit - SAB1300703 from Sigma-Aldrich

Prices $299.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-WDYHV1 (Center) Antibody: <B>Cat. No. SAB1300703</B>: WDYHV1 in human breast carcinoma tissue with Anti-WDYHV1 (Center) Antibody at 40 μg/mL.

WDYHV1 Antibody,FITC conjugated - CSB-PA850306LC01HU from Cusabio

Host Rabbit
Clonality Polyclonal

WDYHV1 Antibody - CSB-PA850306LA01HU from Cusabio

Host Rabbit
Clonality Polyclonal

WDYHV1 Antibody,HRP conjugated - CSB-PA850306LB01HU from Cusabio

Host Rabbit
Clonality Polyclonal

WDYHV1 Antibody,Biotin conjugated - CSB-PA850306LD01HU from Cusabio

Host Rabbit
Clonality Polyclonal

Anti-WDYHV1 Antibody (N-Terminus) - LS-C378872 from LifeSpan Bioscience

Prices $285.00, $385.00
Sizes 50 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western blot. All lanes: WDYHV1 antibody at 6 ug/ml. Lane 1: Mouse heart tissue. Lane 2: MCF7 whole cell lysate. Lane 3: U251 whole cell lysate. Secondary antibody: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 24 kDa. Observed band size: 24 kDa.
Search more