Gene Symbol | WDYHV1 |
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Entrez Gene | 55093 |
Alt Symbol | C8orf32 |
Species | Human |
Gene Type | protein-coding |
Description | WDYHV motif containing 1 |
Other Description | N-terminal Gln amidase|WDYHV motif-containing protein 1|nt(Q)-amidase|protein N-terminal glutamine amidohydrolase|protein NH2-terminal glutamine deamidase |
Swissprots | B4DE68 Q96HA8 Q9NW95 |
Accessions | EAW92037 EAW92038 EAW92039 Q96HA8 AK001066 BAA91488 AK225643 AK293492 BAG56979 AU119047 BC008781 AAH08781 BG528966 HQ448315 ADQ32795 XM_006716597 XP_006716660 XM_011517146 XP_011515448 XM_011517147 XP_011515449 XM_011517148 XP_011515450 XM_011517149 XP_011515451 XM_011517150 XP_011515452 XM_011517151 XP_011515453 XR_928337 XR_928338 NM_001283024 NP_001269953 NM_001283027 NP_001269956 NM_018024 NP_060494 |
Function | Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N- terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C- terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine (By similarity). {ECO:0000250}. |
Subcellular Location | Cytoplasm, cytosol {ECO:0000250}. Nucleus {ECO:0000250}. |
WDYHV1 CRISPR/Cas9 KO Plasmid (h) - sc-412444 from Santa Cruz Biotechnology
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WDYHV1 HDR Plasmid (h) - sc-412444-HDR from Santa Cruz Biotechnology
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WDYHV1 Double Nickase Plasmid (h) - sc-412444-NIC from Santa Cruz Biotechnology
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WDYHV1 Double Nickase Plasmid (h2) - sc-412444-NIC-2 from Santa Cruz Biotechnology
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WDYHV1 CRISPR Activation Plasmid (h) - sc-412444-ACT from Santa Cruz Biotechnology
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WDYHV1 CRISPR Activation Plasmid (h2) - sc-412444-ACT-2 from Santa Cruz Biotechnology
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WDYHV1 Lentiviral Activation Particles (h) - sc-412444-LAC from Santa Cruz Biotechnology
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WDYHV1 Lentiviral Activation Particles (h2) - sc-412444-LAC-2 from Santa Cruz Biotechnology
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WDYHV1 siRNA (h) - sc-77664 from Santa Cruz Biotechnology
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WDYHV1 shRNA Plasmid (h) - sc-77664-SH from Santa Cruz Biotechnology
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WDYHV1 shRNA (h) Lentiviral Particles - sc-77664-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU144851 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22874 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22874 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR04835 from Sigma-Aldrich
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WDYHV1 siRNA (Human) - CRJ0462 from Cohesion Biosciences
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WDYHV1 - MBS8224168 from MyBioSource
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WDYHV1 shRNA Plasmid (Human) - CHJ0462 from Cohesion Biosciences
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WDYHV1 miRNA 3'UTR clone - MiUTR1H-01498 from Creative biogene
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