HERC5
| Gene Symbol | HERC5 |
|---|---|
| Entrez Gene | 51191 |
| Alt Symbol | CEB1, CEBP1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | HECT and RLD domain containing E3 ubiquitin protein ligase 5 |
| Other Description | E3 ISG15--protein ligase HERC5|HECT domain and RCC1-like domain-containing protein 5|cyclin-E-binding protein 1|hect domain and RLD 5|probable E3 ubiquitin-protein ligase HERC5 |
| Swissprots | Q69G20 B2RTQ1 Q9UII4 |
| Accessions | EAX06020 Q9UII4 AB027289 BAA88519 AI336946 AK093155 BAG52666 AK301756 BAG63217 AK302142 BAG63515 AV657231 AY337518 AAR00320 BC140716 AAI40717 XM_011532022 XP_011530324 XM_011532023 XP_011530325 NM_016323 NP_057407 |
| Function | Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant- negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15. {ECO:0000269|PubMed:16407192, ECO:0000269|PubMed:16815975, EC |
| Subcellular Location | Cytoplasm, perinuclear region {ECO:0000269|PubMed:20542004}. Note=Associated with the polyribosomes, probably via the 60S subunit. |
| Tissue Specificity | Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels. {ECO:0000269|PubMed:10581175, ECO:0000269|PubMed:15331633}. |
Herc5 CRISPR/Cas9 KO Plasmid (h) - sc-404993 from Santa Cruz Biotechnology
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Herc5 HDR Plasmid (h) - sc-404993-HDR from Santa Cruz Biotechnology
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Herc5 Double Nickase Plasmid (h) - sc-404993-NIC from Santa Cruz Biotechnology
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Herc5 Double Nickase Plasmid (h2) - sc-404993-NIC-2 from Santa Cruz Biotechnology
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Herc5 CRISPR Activation Plasmid (h) - sc-404993-ACT from Santa Cruz Biotechnology
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Herc5 CRISPR Activation Plasmid (h2) - sc-404993-ACT-2 from Santa Cruz Biotechnology
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Herc5 Lentiviral Activation Particles (h) - sc-404993-LAC from Santa Cruz Biotechnology
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Herc5 Lentiviral Activation Particles (h2) - sc-404993-LAC-2 from Santa Cruz Biotechnology
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Herc5 siRNA (h) - sc-62455 from Santa Cruz Biotechnology
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Herc5 shRNA Plasmid (h) - sc-62455-SH from Santa Cruz Biotechnology
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Herc5 shRNA (h) Lentiviral Particles - sc-62455-V from Santa Cruz Biotechnology
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HERC5 RNAi - H00051191-R01 from Novus Biologicals
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MISSION® esiRNA - EHU007021 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN9986 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND9986 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR02150 from Sigma-Aldrich
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HERC5 siRNA (Human) - CRH9594 from Cohesion Biosciences
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HERC5 - MBS8203798 from MyBioSource
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shRNA set against Human HERC5 (NM_016323.3) - SHH311637 from Creative biogene
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HERC5 shRNA Plasmid (Human) - CHH9594 from Cohesion Biosciences
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HERC5 miRNA 3'UTR clone - MiUTR3H-09865 from Creative biogene
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