POLI

Gene Symbol POLI
Entrez Gene 11201
Alt Symbol RAD30B, RAD3OB
Species Human
Gene Type protein-coding
Description polymerase (DNA directed) iota
Other Description DNA polymerase iota|RAD30 homolog B|eta2|polymerase (DNA-directed), iota
Swissprots Q9NYH6 Q9H0S1 Q8N590 Q9UNA4
Accessions AAM11872 EAW63000 EAW63001 Q9UNA4 AF140501 AAD50381 AF245438 AAF63383 AK093688 AK226057 AK301578 BAH13516 AL136670 CAB66605 AM392978 CAL37856 BC032617 BC032662 AAH32662 BM993293 BX649100 DA315805 DB450447 DQ890893 ABM81819 DQ896811 ABM87810 XM_005258188 XP_005258245 XM_005258191 XP_005258248 XM_005258192 XP_005258249 XM_006722378 XP_006722441 XM_011525796 XP_011524098 XM_011525797 XP_011524099 NM_007195 NP_009126
Function Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. {ECO:0000269|PubMed:11013228, ECO:0000269|PubMed:11251121, ECO:0000269|PubMed:11387224, ECO:0000269|PubMed:12410315, ECO:0000269|PubMed:14630940, ECO:0000269|PubMed:15199127, ECO:0000269|PubMed:15254543}.
Subcellular Location Nucleus {ECO:0000269|PubMed:12606586}. Note=Accumulates at replication forks after DNA damage.
Tissue Specificity Ubiquitous. Highly expressed in testis. {ECO:0000269|PubMed:10458907, ECO:0000269|PubMed:11387224}.
Top Pathways Fanconi anemia pathway

DNA pol ι (K-15) - sc-13832 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

DNA pol ι (N-19) - sc-13830 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

DNA pol ι (NN7) - sc-101026 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
DNA pol ι (NN7): sc-101026. Western blot analysis of DNA pol ι expression in non-transfected 293T: sc-117752 (A), human DNA pol ι transfected 293T: sc-115254 (B) and HeLa (C) whole cell lysates.

Anti-DNA Polymerase iota antibody - ab185686 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DNA Polymerase iota antibody (ab185686) at 1/500 dilutionLane 1 : BT474 cell lysateLane 2 : MCF7 cell lysateLane 3 : mouse testis lysate

Anti-DNA Polymerase iota antibody - ab169928 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Human breast carcinoma tissue labeling DNA Polymerase iota using ab169928 at 1/100 dilution.

Anti-DNA Polymerase iota antibody - ab123331 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-DNA Polymerase iota antibody (ab123331) at 1/100 dilution + HL60 cell line lysates at 35 µg

Anti-DNA Polymerase iota antibody - ab75256 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DNA Polymerase iota antibody [1H9] - ab157244 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H9
All lanes : Anti-DNA Polymerase iota antibody [1H9] (ab157244) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY DNA Polymerase iotaLysates/proteins at 5 µg per lane.

Anti-DNA Polymerase iota antibody - N-terminal - ab104319 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-DNA Polymerase iota antibody - N-terminal (ab104319) at 1 µg/ml + Human fetal lung lysate at 10 µg

Anti-POLI Antibody (aa690-706) - LS-C3374 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-POLI Antibody (aa690-715) - LS-C125466 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-POLI Antibody (aa641-690) - LS-C119910 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HeLa cells, using POLI Antibody. The lane on the right is treated with the synthesized peptide.

Anti-POLI Antibody (aa626-675) - LS-C200505 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HeLa cells using POLI antibody.

Anti-POLI Antibody (aa143-192) - LS-C116474 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
POLI antibody LS-C116474 Western blot of Fetal Lung lysate.

Anti-POLI Antibody (clone 5H9) - LS-C175630 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5H9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLI.

Anti-POLI Antibody (clone 5D11) - LS-C175632 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5D11
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLI.

Anti-POLI Antibody (clone 1H9) - LS-C175629 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLI.

Anti-POLI Antibody (aa673-701, AP) - LS-C284662 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLI Antibody (clone 5A4) - LS-C175633 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5A4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLI.

Anti-POLI Antibody (clone 2C10) - LS-C175634 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2C10
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLI.

Anti-POLI Antibody (aa673-701, APC) - LS-C284663 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLI Antibody (aa673-701, FITC) - LS-C284664 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLI Antibody (clone 4C12) - LS-C175635 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4C12
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLI.

Anti-POLI Antibody (aa673-701, Biotin) - LS-C284665 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLI Antibody (aa673-701, PE) - LS-C284668 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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