TRPV1

Gene Symbol TRPV1
Entrez Gene 7442
Alt Symbol VR1
Species Human
Gene Type protein-coding
Description transient receptor potential cation channel, subfamily V, member 1
Other Description OTRPC1|capsaicin receptor|osm-9-like TRP channel 1|transient receptor potential cation channel subfamily V member 1|transient receptor potential vanilloid 1a|transient receptor potential vanilloid 1b|vanilloid receptor subtype 1
Swissprots Q9H304 A2RUA9 Q8NER1 Q9NY22 Q3LU47 Q9H0G9 Q9NQ74 Q9H303
Accessions EAW90497 EAW90498 EAW90499 EAW90500 Q8NER1 AA700014 AF196175 AAG43466 AF196176 AAG43467 AF235160 AAN73432 AJ272063 CAB89866 AJ277028 CAB95729 AL136801 CAB66735 AM393687 CAL38563 AY131289 AAM89472 AY986821 AAX84657 BC132820 AAI32821 BC136633 AAI36634 BF308801 DQ177332 ABA06605 DQ177333 ABA06606 DQ898279 ABI58280 NM_018727 NP_061197 NM_080704 NP_542435 NM_080705 NP_542436 NM_080706 NP_542437
Function Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Can be activated by endogenous compounds, including 12- hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (By similarity). Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mil
Subcellular Location Cell junction, synapse, postsynaptic cell membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Cell projection, dendritic spine membrane {ECO:0000250}; Multi- pass membrane protein {ECO:0000250}. Cell membrane {ECO:0000269|PubMed:12077606}; Multi-pass membrane protein {ECO:0000269|PubMed:12077606}. Note=Mostly, but not exclusively expressed in postsynaptic dendritic spines. {ECO:0000250}.
Tissue Specificity Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level). {ECO:0000269|PubMed:11050376, ECO:0000269|PubMed:11243859, ECO:0000269|PubMed:14987252}.
Top Pathways Inflammatory mediator regulation of TRP channels, Neuroactive ligand-receptor interaction

VR1 (H-150) - sc-20813 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VR1 (H-150): sc-20813. Western blot analysis of VR1 expression in NIH/3T3 (A), heat shock-treated NIH/3T3 (B), HeLa (C) and heat shock-treated HeLa (D) whole cell lysates.

VR1 (D-20) - sc-12502 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VR1 (D-20): sc-12502. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of keratinocytes, Langerhans cells and melanocytes.

VR1 (N-15) - sc-12500 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VR1 (N-15): sc-12500. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane localization.

VR1 (P-19) - sc-12498 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VR1 (P-19): sc-12498. Western blot analysis of VR1 expression in untreated (A) and heat shock-treated (B) NIH/3T3 whole cell lysates.

VR1 (C-15) - sc-12503 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VR1 (C-15): sc-12503. Western blot analysis of VR1 expression in F9 whole cell lysate.

VR1 (R-130) - sc-28759 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VR1 (R-130): sc-28759. Western blot analysis of VR1 expression in HeLa (A), heat shock induced HeLa (B) and THP-1 (C) whole cell lysates.

Anti-VR1 antibody - ab63083 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab63083 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63083, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-VR1 antibody - ab74855 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab74855 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74855 at 1in100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was ab96899 Dylight® 488 goat anti-rabbit IgG  (H+L) preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-VR1 antibody - ab3487 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (frozen sections) analysis of human skin tissue sections labeling VR1 (red) with ab3487 at 1/250. A biotin-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

Anti-VR1 antibody - ab74813 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab74813 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74813 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-VR1 antibody - ab111973 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VR1 antibody (ab111973) at 1 µg/mlLane 1 : Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 10 µgLane 2 : Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-VR1 antibody - ab63103 from Abcam

Host Sheep
Clonality Polyclonal

Anti-VR1 antibody - ab74812 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-VR1 antibody - ab130438 from Abcam

Host Chicken
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody (C-Terminus) - LS-C21193 from LifeSpan Bioscience

Prices $515.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody (C-Terminus) - LS-C21194 from LifeSpan Bioscience

Prices $385.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody (C-Terminus) - LS-C21195 from LifeSpan Bioscience

Prices $415.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody - LS-C21197 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody (C-Terminus) - LS-C21198 from LifeSpan Bioscience

Prices $1,110.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody (C-Terminus) - LS-C21199 from LifeSpan Bioscience

Prices $560.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody (C-Terminus) - LS-C21200 from LifeSpan Bioscience

Prices $530.00
Sizes 50 µl
Host Guinea Pig
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody (aa817-838) - LS-C21202 from LifeSpan Bioscience

Prices $405.00
Sizes 50 µl
Host Guinea Pig
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody (N-Terminus) - LS-C21203 from LifeSpan Bioscience

Prices $405.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody (aa531-541) - LS-C77694 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-VR1 / TRPV1 Antibody (aa608-621) - LS-C77695 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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