SPC25

Gene Symbol SPC25
Entrez Gene 57405
Alt Symbol AD024, SPBC25, hSpc25
Species Human
Gene Type protein-coding
Description SPC25, NDC80 kinetochore complex component
Other Description 2600017H08Rik|SPC25, NDC80 kinetochore complex component, homolog|kinetochore protein Spc25|spindle pole body component 25 homolog
Swissprots Q9HBM1 A8K4X8 D3DPC0
Accessions AAY24098 EAX11288 EAX11289 EAX11290 Q9HBM1 AF225416 AAG09718 AK291093 BAF83782 AY456388 AAR88652 BC022255 AAH22255 CR457197 CAG33478 HQ447658 ADQ32143 XM_011511516 XP_011509818 XM_011546938 XP_011545240 XM_011546939 XP_011545241 XM_011546940 XP_011545242 NM_020675 NP_065726
Function Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. {ECO:0000269|PubMed:14699129, ECO:0000269|PubMed:14738735}.
Subcellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.

Spc25 (C-15) - sc-69332 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Spc25 (C-15): sc-69332. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing nuclear staining of cells in germinal centers and cells in non-germinal centers.

Spc25 (L-18) - sc-69334 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Spc25 (T-15) - sc-69335 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-SPC25 antibody - ab20679 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab20679 (1/200) staining SPC25 in assynchronous HeLa cells, in conjunction with a secondary goat anti-rabbit antibody conjugated to Cy3® (green). Cells were counterstained with DAPI to show the nucleus (red).See Abreview

Anti-SPC25 antibody - ab89028 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-SPC25 antibody (ab89028) at 1/500 dilution + human kidney tissue lysate at 50 µg

Anti-SPC25 antibody - ab116328 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-SPC25 antibody (ab116328) at 1 µg/ml + 721_B cell lysate at 10 µg

Anti-SPC25 Antibody - LS-C109454 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-SPC25 Antibody (aa87-114, AP) - LS-C270132 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPC25 Antibody (aa87-114, APC) - LS-C270134 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPC25 Antibody (aa87-114, Biotin) - LS-C270135 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPC25 Antibody (aa87-114, Azide-free, HRP) - LS-C270136 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPC25 Antibody (aa87-114, FITC) - LS-C270137 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPC25 Antibody (aa87-114, PE) - LS-C270138 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPC25 Antibody (aa79-128) - LS-C145476 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SPC25 antibody LS-C145476 Western blot of 721_B Cell lysate. Antibody concentration 1 ug/ml.

Anti-SPC25 Antibody (aa86-114) - LS-C325319 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SPC25 Antibody (clone 3D11) - LS-C340003 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3D11
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPC25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPC25.

Anti-SPC25 Antibody (aa87-114) - LS-C157073 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SPC25 Antibody western blot of ZR-75-1 cell line lysates (35 ug/lane). The SPC25 antibody detected the SPC25 protein (arrow).

Anti-SPC25 Antibody (clone 1F8) - LS-C340004 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F8
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPC25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPC25.

Anti-SPC25 Antibody (clone 2B7) - LS-C340005 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B7
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPC25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPC25.

Anti-SPC25 Antibody (clone 5E7) - LS-C340008 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5E7
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPC25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPC25.

Anti-SPC25 Antibody (clone 3A8) - LS-C340006 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3A8
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPC25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPC25.

SPC25 Antibody - NBP1-31580 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SPC25 Antibody [NBP1-31580] - Sample(30 ug whole cell lysate)A:A431 B:H1299 7.5% SDS PAGE, antibody diluted at 1:1000.

SPC25 Antibody - H00057405-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

SPC25 Polyclonal Antibody - bs-7716R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ANTI-SPC25 (CENTER) antibody produced in rabbit - SAB1304490 from Sigma-Aldrich

Prices $326.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SPC25 (Center): <B>Cat. No. SAB1304490</B>: SPC25 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35 μg/lane).
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