PREB

Gene Symbol	PREB
Entrez Gene	10113
Alt Symbol	SEC12
Species	Human
Gene Type	protein-coding
Description	prolactin regulatory element binding
Other Description	mammalian guanine nucleotide exchange factor mSec12\|prolactin regulatory binding-element protein\|prolactin regulatory element-binding protein
Swissprots	Q53SZ8 Q9HCU5 Q9UH94
Accessions	AAF74572 AAX93170 EAX00626 EAX00627 EAX00628 EAX00629 EAX00630 Q9HCU5 AA629936 AF203687 AAF19192 AF226684 AAG01692 AK001976 AK023064 BAB14385 AK023405 AK292178 BAF84867 BC002765 AAH02765 BC008658 BC012890 AAH12890 BC016472 AAH16472 BC016906 AAH16906 BC036185 BC041032 AAH41032 BG715668 DQ892120 ABM83046 XM_006711914 XP_006711977 XM_011532471 XP_011530773 XM_011532472 XP_011530774 XR_939648 XR_939649 NM_013388 NP_037520
Function	Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription (By similarity). Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER. {ECO:0000250}.
Subcellular Location	Nucleus {ECO:0000250}. Endoplasmic reticulum membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
Tissue Specificity	Ubiquitous. {ECO:0000269\|PubMed:10920239, ECO:0000269\|PubMed:12735795}.
Top Pathways	Protein processing in endoplasmic reticulum

PREB (S-13) - sc-107967 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-PREB antibody - ab42501 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PREB antibody (ab42501) at 0.5 µg/ml + Raji cell lysateSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PREB antibody - ab177045 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue labeling PREB with ab177045 at 1/100 dilution.

Anti-PREB antibody [EPR13252] - ab181212 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13252

Western blot analysis of immunoprecipitation pellet from Raji cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181212 at 1/50 dilution.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Anti-PREB antibody - N-terminal - ab171064 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PREB antibody - N-terminal (ab171064) at 1 µg/ml + Mouse liver lysate at 10 µg

Anti-PREB Antibody - LS-C9686 from LifeSpan Bioscience

Prices	$530.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PREB Antibody (aa137-384) - LS-C311850 from LifeSpan Bioscience

Prices	$250.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PREB Antibody (aa140-189) - LS-C29886 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

PREB antibody LS-C29886 was used in IHC to stain formalin-fixed, paraffin-embedded human heart.

Anti-PREB Antibody (N-Terminus) - LS-C170288 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

PREB Antibody - AF5557 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Goat
Clonality	Polyclonal

Western blot shows lysates of human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti‑Human PREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5557) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PREB at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

PREB Antibody - NBP1-87056 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

PREB Antibody - NBP1-87057 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: PREB Antibody [NBP1-87057] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).

PREB Antibody - H00010113-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Anti-PREB antibody produced in rabbit - HPA013582 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-PREB: <B>Cat. No. HPA013582</B>: Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Anti-PREB antibody produced in rabbit - HPA014133 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-PREB: <B>Cat. No. HPA014133</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

PREB antibody - N-terminal region (ARP34130_P050) - ARP34130_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Human PREB Antibody - AF5557 from R&D Systems

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Goat

Detection of Human PREB by Western Blot. Western blot shows lysates of human tonsil tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PREB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5557) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019 ). A specific band was detected for PREB at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .

Human PREB Affinity Purified Polyclonal Ab, Goat IgG - AF5557-SP from R&D Systems

Host	Goat
Clonality	Polyclonal

PREB antibody - 70R-8016 from Fitzgerald

Prices	$345.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

PREB antibody - 70R-19506 from Fitzgerald

Prices	$375.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

PREB Antibody - abx036141 from Abbexa

Host	Rabbit
Clonality	Polyclonal

PREB antibody - 10146-2-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 10146-2-AP( PREB Antibody) at dilution of 1:50 (under 10x lens)

PREB antibody - H00010113-A01 from Acris Antibodies

Prices	$300.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

PREB Antibody - 28-010 from ProSci

Prices	$315.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

PREB antibody - 10146-2-AP from Proteintech Group

Prices	$279.00
Sizes	con: 59 μg/150μl
Host	Rabbit
Clonality	Polyclonal

WB result of anti-PREB (10146-2-AP, 1:250) with HepG2 and HT1080 by Dr. Dzmitry Batrakou.

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