PRKAA1

Gene Symbol PRKAA1
Entrez Gene 5562
Alt Symbol AMPK, AMPKa1
Species Human
Gene Type protein-coding
Description protein kinase, AMP-activated, alpha 1 catalytic subunit
Other Description 5'-AMP-activated protein kinase catalytic subunit alpha-1|5'-AMP-activated protein kinase, catalytic alpha-1 chain|ACACA kinase|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1|AMPK subunit alpha-1|HMGCR kinase|acetyl-CoA carboxylase kinase|hydroxymethylglutaryl-CoA reductase kinase|tau-protein kinase PRKAA1
Swissprots Q86VS1 Q5D0E1 O00286 Q9UNQ4 A8MTQ6 Q13131 B2R7E1
Accessions EAW56000 EAW56001 Q13131 AB022017 BAA36547 AF100763 AAD43027 AK024252 AK307546 AK312947 BAG35788 BC012622 AAH12622 BC037303 AAH37303 BC048980 AAH48980 BQ049192 BQ716041 BX494823 CD512588 DB236105 JF432366 ADZ15583 U22456 AAA64850 Y12856 CAA73361 XM_006714482 XP_006714545 NM_006251 NP_006242 NM_206907 NP_996790
Function Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphoryl
Subcellular Location Cytoplasm {ECO:0000269|PubMed:15866171}. Nucleus {ECO:0000269|PubMed:15866171}. Note=In response to stress, recruited by p53/TP53 to specific promoters.
Top Pathways Glucagon signaling pathway, AMPK signaling pathway, Oxytocin signaling pathway, Non-alcoholic fatty liver disease (NAFLD), PI3K-Akt signaling pathway

Phospho-AMPKα (Thr172) (40H9) Rabbit mAb (Biotinylated) - 5256 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 40H9
Western blot analysis of extracts from C2C12 cells, untreated (-) or hydrogen peroxide-treated (+), using Phospho-AMPKα (Thr172) (40H9) Rabbit mAb (Biotinylated).

AMPKα (D5A2) Rabbit mAb (Biotinylated) - 4811 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5A2
Western blot analysis of extracts from HeLa and C2C12 cells using AMPKα (D5A2) Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for detection.

Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb - 4188 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (20 western blots), 300 µl (60 western blots)
Host Rabbit
Clonality Monoclonal
Clone D79.5E
Western analysis of extracts from SK-N-MC cells, untreated or AICAR-treated, using Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb (upper) or AMPKα Antibody #2532 (lower).

Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb - 2537 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 45F5
Western blot analysis of HEK293 cell lysates treated with λ phosphatase or AICAR, using Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb (upper) and AMPKα Antibody #2532 (lower).

Phospho-AMPKα1 (Ser485) Antibody - 4184 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HEK293 and C6 cells, λ phosphatase-treated or oligomycin-treated, using Phospho-AMPKα1 (Ser485) Antibody (upper) or AMPKα Antibody #2532 (lower).

Phospho-AMPKα (Thr172) (40H9) Rabbit mAb - 2535 from Cell Signaling Technology

Prices $109.00, $307.00, $719.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 40H9
Western blot analysis of extracts from C2C12 cells, untreated or oligomycin-treated (0.5 µM), using Phospho-AMPKα (Thr172) (40H9) Rabbit mAb (upper) or AMPKα Antibody #2532 (lower).

Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody - 4185 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HEK293 and C6 cells, λ phosphatase-treated or oligomycin-treated, using Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody (upper) or AMPKα Antibody #2532 (lower).

Phospho-AMPKα (Thr172) Antibody - 2531 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of untreated and AICAR-treated C2C12 cell extracts using Phospho-AMPKα (Thr172) Antibody.

AMPKα (23A3) Rabbit mAb - 2603 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 23A3
Western blot analysis of extracts from 293, COS, mouse brain and PC12 cells, using AMPKα (23A3) Rabbit mAb.

AMPKα Antibody - 2532 from Cell Signaling Technology

Prices $246.00, $596.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HEK293 cells, untreated or oligomycin-treated (0.5μM) for the indicated times, using AMPKα Antibody.

AMPKα (D5A2) Rabbit mAb - 5831 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5A2
Western blot analysis of extracts from HeLa, K-562, C6, and Neuro-2a cells using AMPKα (D5A2) Rabbit mAb.

AMPKα1 Antibody - 2795 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, HT29 and COS cells, using AMPKα1 Antibody.

AMPKα (F6) Mouse mAb - 2793 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone F6
Western blot analysis of extracts from various cell types using AMPKα (F6) Mouse mAb.

AMPKα (D63G4) Rabbit mAb - 5832 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D63G4
Western blot analysis of extracts from HeLa, K-562, C6, and Neuro-2a cells using AMPKα (D63G4) Rabbit mAb.

AMPKα1 (H-4) - sc-398861 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AMPK1 (H-4): sc-398861. Western blot analysis of AMPK1 expression in IB4 (A), MCF7 (B), Jurkat (C), HeLa (D) and K-562 (E) whole cell lysates.

AMPKα1 (C-20) - sc-19128 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AMPKα1 (C-20): sc-19128. Western blot analysis of AMPKα1 expression in Jurkat (A) and IB4 (B) whole cell lysates.

AMPKα1 (D-19) - sc-19126 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

AMPKα1/2 (D-6) - sc-74461 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AMPKα1/2 (D-6): sc-74461. Western blot analysis of AMPKα1/2 expression in HeLa (A), Hep G2 (B), MCF7 (C), K-562 (D) and MDCK (E) whole cell lysates.

AMPKα1/2 (H-300) - sc-25792 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
AMPKα1/2 (H-300): sc-25792. Immunofluorescence staining of methanol-fixed L8 cells showing cytoplasmic localization.

AMPKα1 (71.54) - sc-130394 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of AMPKα1/2 phosphorylation in untreated (A,C) and lambda protein phosphatase treated (B,D) C2C12 whole cell lysates. Antibodies tested include p-AMPKα1/2 (Thr 172): sc-33524 (A,C) and AMPKα1 (71.54): sc-130394 (B,D).

p-AMPKα1 (Ser 485) - sc-109907 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

p-AMPKα1/2 (Thr 172) - sc-33524 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of AMPKα1/2 phosphorylation in untreated (A,C) and lambda protein phosphatase treated (B,D) C2C12 whole cell lysates. Antibodies tested include p-AMPKα1/2 (Thr 172): sc-33524 (A,C) and AMPKα1 (71.54): sc-130394 (B,D).

p-AMPKα1/2 (Thr 183/172) - sc-101630 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-AMPKα1/2 (Thr 183/172). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic localization.

Anti-AMPK alpha 1 + AMPK alpha 2 antibody - ab131512 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol fixed HeLa cells labelling AMPK alpha 1 + AMPK alpha 2 with ab131512 at 1/100 dilution.

Anti-AMPK alpha 1 + AMPK alpha 2 antibody - ab39644 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab39644, at 1/50 dilution, staining AMPK alpha 1 + AMPK alpha 2 in Human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue.
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