| Gene Symbol | DPPA3 |
|---|---|
| Entrez Gene | 359787 |
| Alt Symbol | STELLA |
| Species | Human |
| Gene Type | protein-coding |
| Description | developmental pluripotency associated 3 |
| Other Description | developmental pluripotency-associated protein 3|stella-related protein |
| Swissprots | Q0P5U3 Q6JZS6 Q6W0C5 |
| Accessions | EAW88657 EAW88658 Q6W0C5 AY317075 AAQ84110 BC160067 AAI60067 NM_199286 NP_954980 |
| Function | Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. A |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:21421998}. Cytoplasm {ECO:0000269|PubMed:21421998}. Note=Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker. {ECO:0000250}. |
| Tissue Specificity | Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors. {ECO:0000269|PubMed:14654002, ECO:0000269|PubMed:14684992, ECO:0000269|PubMed:14990856}. |
STELLA CRISPR/Cas9 KO Plasmid (h) - sc-403261 from Santa Cruz Biotechnology
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STELLA HDR Plasmid (h) - sc-403261-HDR from Santa Cruz Biotechnology
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STELLA Double Nickase Plasmid (h) - sc-403261-NIC from Santa Cruz Biotechnology
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STELLA Double Nickase Plasmid (h2) - sc-403261-NIC-2 from Santa Cruz Biotechnology
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STELLA CRISPR Activation Plasmid (h) - sc-403261-ACT from Santa Cruz Biotechnology
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STELLA CRISPR Activation Plasmid (h2) - sc-403261-ACT-2 from Santa Cruz Biotechnology
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STELLA Lentiviral Activation Particles (h) - sc-403261-LAC from Santa Cruz Biotechnology
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STELLA Lentiviral Activation Particles (h2) - sc-403261-LAC-2 from Santa Cruz Biotechnology
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STELLA siRNA (h) - sc-72248 from Santa Cruz Biotechnology
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STELLA shRNA Plasmid (h) - sc-72248-SH from Santa Cruz Biotechnology
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STELLA shRNA (h) Lentiviral Particles - sc-72248-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU106871 from Sigma-Aldrich
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MISSION® esiRNA - EHU106221 from Sigma-Aldrich
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MISSION® esiRNA - EHU112011 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN7465 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND7465 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR04814 from Sigma-Aldrich
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DPPA3 siRNA (Human) - CRJ7597 from Cohesion Biosciences
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DPPA3 - MBS8237436 from MyBioSource
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shRNA set against Human DPPA3 (NM_199286.2) - SHH280745 from Creative biogene
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shRNA set against Human DPPA3(NM_199286.2) - SHH014851 from Creative biogene
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Human DPPA3 siRNA - orb278098 from Biorbyt
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DPPA3 shRNA Plasmid (Human) - CHJ7597 from Cohesion Biosciences
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DPPA3 miRNA 3'UTR clone - MiUTR1H-02982 from Creative biogene
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