Dorso-ventral axis formation



PathScan ® Phospho-EGF Receptor (Tyr1068) Sandwich ELISA Kit - 7240 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of A431 cells with EGF stimulates phosphorylation of EGF Receptor at Tyr1068, detected by PathScan® Phospho-EGF Receptor (Tyr1068) Sandwich ELISA kit #7240, but does not affect the level of total EGF Receptor detected by PathScan® Total EGF Receptor Sandwich ELISA kit #7250. OD 450 nm readings are shown in the top figure, while the corresponding Western blot using Phospho-EGF Receptor (Tyr1068) Antibody #2234 (right panel) or EGF Receptor Antibody #2232 (left panel), is shown in the bottom figure.

PathScan ® Phospho-EGF Receptor (Tyr845) Sandwich ELISA Kit - 7189 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 2: The relationship between protein concentration of lysates from untreated and EGF-treated A431 cells and kit assay optical density readings. After starvation, A431 cells (85% confluence) were treated with EGF (100 ng/ml) for 5 min at 37°C, and then lysed.

PathScan ® Phospho-EGF Receptor (Tyr1173) Sandwich ELISA Kit - 7187 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 2: The relationship between protein concentration of lysates from untreated and EGF-treated A431 cells and kit assay optical density readings. After starvation, A431 cells (85% confluence) were treated with EGF (100 ng/ml) for 5 min at 37°C, and then lysed.

PathScan ® Phospho-EGF Receptor (Tyr1068) Chemiluminescent Sandwich ELISA Kit - 7036 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. The relationship between protein concentration of lysates from A-431 cells, untreated or treated with hEGF #8916, and immediate light generation with chemiluminescent substrate. After starvation, A-431 cells (85% confluence) were treated with EGF (100 ng/ml, 5 min at 37°C) and then lysed. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® Phospho-EGF Receptor (panTyr) Sandwich ELISA Kit - 7911 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. Treatment of A-431 cells with hEGF #8916 stimulates tyrosine phosphorylation of EGF receptor detected by PathScan ® Phospho-EGF Receptor (panTyr) Sandwich ELISA Kit #7911, but does not affect the level of total EGF receptor detected by PathScan ® Total EGF Receptor Sandwich ELISA Kit #7250. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using EGF Receptor Antibody #2232 (left panel) or Phospho-EGF Receptor (Tyr1173) (53A2) Rabbit mAb #4407 (right panel) are shown in the bottom figure.

PathScan ® Total EGF Receptor Sandwich ELISA Kit - 7250 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 2: The relationship between protein concentration of lysates from untreated and EGF-treated A431 cells and kit assay optical density readings. After starvation, A431 cells (85% confluence) were treated with EGF (100 ng/ml) for 5 min at 37°C, and then lysed.

PathScan ® Total EGF Receptor Chemiluminescent Sandwich ELISA Kit - 7297 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. The relationship between protein concentration of lysates from A-431 cells, untreated or treated with hEGF #8916, and immediate light generation with chemiluminescent substrate. After starvation, A-431 cells (85% confluence) were treated with hEGF (100 ng/ml, 5 min at 37°C) and then lysed. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan ® EGF Receptor (E746-A750del Specific) Sandwich ELISA Kit - 8950 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)

PathScan ® EGF Receptor (L858R Mutant Specific) Sandwich ELISA Kit - 8951 from Cell Signaling Technology

Category ELISA Kit

HTScan® EGF Receptor Kinase Assay Kit - 7909 from Cell Signaling Technology

Category Assay Kit
Figure 1. EGFR kinase activity was measured in a radiometric assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 5 mM MgCl2, 5 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, 100 µM ATP, 250 µM PTP1B (Tyr66) Biotinylated Peptide (#1325) and variable amount of Recombinant EGFR. Reaction mixture incubated at room temperature for 10 minutes.

HTScan® EGFR Kinase Assay Kit - 7410 from Cell Signaling Technology

Category Assay Kit
Figure 5. EGF-R kinase activity was measured in a radioisotopic filtration assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 uM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 ug / 50 ul PEG20.000, Substrate:PolyEY, 10 ug/50 ul, Recombinant EGF-R: 5 Units/50 ul.

EGFR (ERBB) Human ELISA Kit - ab100505 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
Sensitivity < 4 pg/ml
Range 2.74 pg/ml - 2000 pg/ml
EGFR measured in cell lysates showing quantity (ng) per 1 million cells

EGFR (pS1070) + total EGFR Human ELISA Kit - ab126435 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pS1070) Human ELISA Kit - ab126434 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pY1045) + total EGFR Human ELISA Kit - ab126437 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 2 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pY1045) Human ELISA Kit - ab126436 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 2 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pY1068) + total EGFR Human ELISA Kit - ab126439 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 10 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126439.

EGFR (pY1068) Human ELISA Kit - ab126438 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 10 min. Cells were solubilized at 4 x 107 cells/ml in Lysis Buffer. Serial dilutions of lysates were analyzed using ab126438.

EGFR (pY1086) + total EGFR Human ELISA Kit - ab126441 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pY1086) Human ELISA Kit - ab126440 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126440.

EGFR (pY992) + total EGFR Human ELISA Kit - ab126443 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.

EGFR (pY992) Human ELISA Kit - ab126442 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126442.

EGFR (Tyr1068) Human In-Cell ELISA Kit - ab126420 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were stimulated by different concentrations of EGF for 10 min at 37°C.

EGFR (Tyr845) In-Cell ELISA Kit - ab126421 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests
A431 cells were stimulated by different concentrations of EGF for 10 min at 37°C.
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