MED1

Gene Symbol	MED1
Entrez Gene	5469
Alt Symbol	CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2
Species	Human
Gene Type	protein-coding
Description	mediator complex subunit 1
Other Description	ARC205\|PPAR binding protein\|PPAR-binding protein\|PPARG binding protein\|TR-interacting protein 2\|TRIP-2\|activator-recruited cofactor 205 kDa component\|mediator of RNA polymerase II transcription subunit 1\|p53 regulatory protein RB18A\|peroxisome proliferator-activated receptor-binding protein\|thyroid hormone receptor-associated protein complex 220 kDa component\|thyroid hormone receptor-associated protein complex component TRAP220\|thyroid receptor interacting protein 2\|thyroid receptor-interacting protein 2\|vitamin D receptor-interacting protein 230 kD\|vitamin D receptor-interacting protein complex component DRIP205
Swissprots	O75447 O43810 A2RRQ6 Q9HD39 Q6PK58 Q6P9H7 Q15648
Accessions	EAW60575 Q15648 AF055994 AAC39854 AF283812 AAF98352 AK299789 BAG61669 AK314185 BAG36866 BC006517 AAH06517 BC060758 AAH60758 BC131783 AAI31784 BP357374 JF432429 ADZ15646 L40366 AAC41736 Y13467 CAA73867 XM_005257465 XP_005257522 XM_006721957 XP_006722020 NM_004774 NP_004765
Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. {ECO:0000269\|PubMed:10406464, ECO:0000269\|PubMed:11867769, ECO:0000269\|PubMed:12037571, ECO:0000269\|PubMed:12218053, ECO:0000269\|PubMed:12556447, ECO:0000269\|PubMed:14636573, ECO:0000269\|PubMed:15340084, ECO:0000269\|PubMed:15471764, ECO:0000269\|PubMed:15989967, ECO:0000269\|PubMed:16574658, ECO:0000269\|PubMed:9653119}.
Subcellular Location	Nucleus {ECO:0000269\|PubMed:15471764, ECO:0000269\|PubMed:16314496, ECO:0000269\|PubMed:16574658}. Note=A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
Tissue Specificity	Ubiquitously expressed. {ECO:0000269\|PubMed:9444950, ECO:0000269\|PubMed:9653119}.

TRAP220 (C-19) - sc-5334 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

TRAP220 (C-19): sc-5334. Western blot analysis of TRAP220 expression in HeLa nuclear extract.

TRAP220 (H-7) - sc-74475 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

TRAP220 (H-7): sc-74475. Western blot analysis of TRAP220 expression in Jurkat (A) and Sol8 (B) whole cell lysates.

TRAP220 (M-255) - sc-8998 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

TRAP220 (M-255): sc-8998. Western blot analysis of TRAP220 expression in Sol8 whole cell lysate.

TRAP220 (S-19) - sc-5335 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

TRAP220 (S-19): sc-5335. Western blot analysis of TRAP220 expression in Jurkat whole cell lysate.

Anti-TRAP220/MED1 (phospho T1457) antibody - ab182660 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-TRAP220/MED1 (phospho T1457) antibody (ab182660) at 1/500 dilutionLane 1 : HUVEC cell extract, treated with serum with antigen-specific peptideLane 2 : HUVEC cell extract, treated with serum

Anti-TRAP220/MED1 (phospho T1457) antibody [EPR13269] - ab181103 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR13269

Immunohistochemical analysis of paraffin-embedded sections of Human thyroid gland carcinoma tissue labeling TRAP220/MED1 (phospho T1457) using ab181103 at a 1/250 dilution, followed by a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin.

Anti-TRAP220/MED1 (phospho T1457) antibody - ChIP Grade - ab60950 from Abcam

Prices	$401.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ab60950 at 1/50 - 1/100 dilution staining TRAP220/MED1 in human heart by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide

Anti-TRAP220/MED1 antibody - ab64965 from Abcam

Prices	$384.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-TRAP220/MED1 antibody (ab64965) at 1/500 dilutionLane 1 : extracts from Jurkat cells, without immunising peptideLane 2 : extracts from Jurkat cells, with immunising peptide

Anti-TRAP220/MED1 antibody - ab82461 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-TRAP220/MED1 antibody (ab82461) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-TRAP220/MED1 antibody - ab52065 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-TRAP220/MED1 antibody - ab58140 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Western blot against tagged recombinant protein immunogen using ab58140 TRAP220/MED1 antibody at 1ug/ml.

Anti-TRAP220/MED1 antibody - ab84386 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

ab84386 at 1/250 dilution staining TRAP220/MED1 in human breast tumor by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

Anti-TRAP220/MED1 antibody - ab61119 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ab61119, at a 1/50 dilution, staining human TRAP220/MED1 in HeLa cells with (+) or without (-) immunising peptide, using Immunofluorescence

Anti-MED1 / TRAP220 Antibody - LS-C192869 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts of Jurkat cell lines, using MED1 antibody.

Anti-MED1 / TRAP220 Antibody (aa1531-1581) - LS-C290523 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Goat
Clonality	Polyclonal

Detection of Human and Mouse CRSP1/TRAP220 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibodies: Affinity purified goat anti-CRSP1/TRAP220 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-MED1 / TRAP220 Antibody - LS-C331656 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts of Jurkat cell lines, using MED1 antibody.

Anti-MED1 / TRAP220 Antibody (phospho-Thr1457) - LS-C199486 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of paraffin-embedded human heart, using PPAR-BP (Phospho-Thr1457) Antibody. The sample on the right was incubated with synthetic peptide.

Anti-MED1 / TRAP220 Antibody (Pro697) - LS-C176817 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of TRAP220 (P697) pAb in paraffin-embedded human breast carcinoma tissue.

Anti-MED1 / TRAP220 Antibody (clone 2A2) - LS-C197960 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2A2

Immunofluorescence of monoclonal antibody to PPARBP on HeLa cell . [antibody concentration 10 ug/ml]

Anti-MED1 / TRAP220 Antibody - LS-C48658 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED1 / TRAP220 Antibody (Ser1451) - LS-C191909 from LifeSpan Bioscience

Prices	$345.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-MED1 / TRAP220 Antibody (aa31-358) - LS-C311817 from LifeSpan Bioscience

Prices	$250.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED1 / TRAP220 Antibody (aa1423-1472) - LS-C117699 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence of HeLa cells, using PPAR-BP (Ab-1457) Antibody. The picture on the right is treated with the synthesized peptide.

Anti-MED1 / TRAP220 Antibody (aa641-690) - LS-C119067 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of extracts from Jurkat cells, using MED1 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-MED1 / TRAP220 Antibody (C-Terminus) - LS-C286416 from LifeSpan Bioscience

Prices	$415.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Mouse CRSP1(TRAP220) by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-CRSP1(TRAP220) used at a dilution of 1:250.

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