Gene Symbol | DNA2 |
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Entrez Gene | 1763 |
Alt Symbol | DNA2L, hDNA2 |
Species | Human |
Gene Type | protein-coding |
Description | DNA replication helicase/nuclease 2 |
Other Description | DNA replication ATP-dependent helicase-like homolog|DNA replication ATP-dependent helicase/nuclease DNA2|DNA replication helicase 2 homolog|DNA2-like helicase |
Swissprots | Q8N346 Q7Z6H9 Q6PI80 Q5TC49 P51530 Q2NKM1 Q5TC50 Q6P455 |
Accessions | P51530 AK307939 AK308333 AK308358 BC017003 BC028188 AAH28188 BC041115 AAH41115 BC053574 AAH53574 BC063664 AAH63664 BC073945 BC111740 AAI11741 D42046 BAA07647 DC404138 XM_006717680 XP_006717743 XM_011539416 XP_011537718 XM_011539417 XP_011537719 XM_011539418 XP_011537720 XM_011539419 XP_011537721 NM_001080449 NP_001073918 NR_102264 |
Function | Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double- strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)- dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. Accord |
Subcellular Location | Nucleus. Mitochondrion. Note=Was initially reported to be exclusively mitochondrial (PubMed:18995831). However, it was later shown to localize both in mitochondrion and nucleus (PubMed:19487465). {ECO:0000269|PubMed:18995831, ECO:0000269|PubMed:19487465}. |
Top Pathways | DNA replication |
DNA2L CRISPR/Cas9 KO Plasmid (h) - sc-407704 from Santa Cruz Biotechnology
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DNA2L HDR Plasmid (h) - sc-407704-HDR from Santa Cruz Biotechnology
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DNA2L Double Nickase Plasmid (h) - sc-407704-NIC from Santa Cruz Biotechnology
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DNA2L Double Nickase Plasmid (h2) - sc-407704-NIC-2 from Santa Cruz Biotechnology
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DNA2L CRISPR Activation Plasmid (h) - sc-407704-ACT from Santa Cruz Biotechnology
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DNA2L CRISPR Activation Plasmid (h2) - sc-407704-ACT-2 from Santa Cruz Biotechnology
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DNA2L Lentiviral Activation Particles (h) - sc-407704-LAC from Santa Cruz Biotechnology
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DNA2L Lentiviral Activation Particles (h2) - sc-407704-LAC-2 from Santa Cruz Biotechnology
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DNA2L siRNA (h) - sc-90458 from Santa Cruz Biotechnology
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DNA2L shRNA Plasmid (h) - sc-90458-SH from Santa Cruz Biotechnology
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DNA2L shRNA (h) Lentiviral Particles - sc-90458-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU076881 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN7326 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND7326 from Sigma-Aldrich
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DNA2 siRNA (Human) - CRH1226 from Cohesion Biosciences
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DNA2 - MBS8209291 from MyBioSource
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shRNA set against Human DNA2 (NM_001080449.2) - SHH279037 from Creative biogene
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Human DNA2 siRNA - orb261728 from Biorbyt
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DNA2 shRNA Plasmid (Human) - CHH1226 from Cohesion Biosciences
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DNA2 miRNA 3'UTR clone - MiUTR3H-04430 from Creative biogene
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