Gene Symbol | HCRT |
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Entrez Gene | 3060 |
Alt Symbol | NRCLP1, OX, PPOX |
Species | Human |
Gene Type | protein-coding |
Description | hypocretin (orexin) neuropeptide precursor |
Other Description | orexin|prepro-orexin |
Swissprots | O43612 |
Accessions | AAD24459 EAW60810 O43612 AF041240 AAC39600 BC111915 AAI11916 NM_001524 NP_001515 |
Function | Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. |
Subcellular Location | Rough endoplasmic reticulum {ECO:0000250}. Cytoplasmic vesicle {ECO:0000250}. Cell junction, synapse {ECO:0000250}. Note=Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. {ECO:0000250}. |
Tissue Specificity | Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
Orexin-A CRISPR/Cas9 KO Plasmid (h) - sc-400357 from Santa Cruz Biotechnology
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Orexin-A HDR Plasmid (h) - sc-400357-HDR from Santa Cruz Biotechnology
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Orexin-A Double Nickase Plasmid (h) - sc-400357-NIC from Santa Cruz Biotechnology
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Orexin-A Double Nickase Plasmid (h2) - sc-400357-NIC-2 from Santa Cruz Biotechnology
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Orexin-A CRISPR Activation Plasmid (h) - sc-400357-ACT from Santa Cruz Biotechnology
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Orexin-A CRISPR Activation Plasmid (h2) - sc-400357-ACT-2 from Santa Cruz Biotechnology
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Orexin-A Lentiviral Activation Particles (h) - sc-400357-LAC from Santa Cruz Biotechnology
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Orexin-A Lentiviral Activation Particles (h2) - sc-400357-LAC-2 from Santa Cruz Biotechnology
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Orexin-A/B siRNA (h) - sc-42152 from Santa Cruz Biotechnology
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Orexin-A/B shRNA Plasmid (h) - sc-42152-SH from Santa Cruz Biotechnology
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Orexin-A/B shRNA (h) Lentiviral Particles - sc-42152-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN9929 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND9929 from Sigma-Aldrich
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HCRT siRNA (Human) - CRH2082 from Cohesion Biosciences
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HCRT - MBS8227805 from MyBioSource
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Orexin-A CRISPR/Cas9 KO Plasmid (h2) - sc-400357-KO-2 from Santa Cruz Biotechnology
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Orexin-A HDR Plasmid (h2) - sc-400357-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human HCRT (NM_001524.1) - SHH310885 from Creative biogene
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shRNA set against Human HCRT(NM_001524.1) - SHH115895 from Creative biogene
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Human HCRT siRNA - orb262584 from Biorbyt
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HCRT shRNA Plasmid (Human) - CHH2082 from Cohesion Biosciences
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HCRT miRNA 3'UTR clone - MiUTR1H-04410 from Creative biogene
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