IRAK1

Gene Symbol IRAK1
Entrez Gene 3654
Alt Symbol IRAK, pelle
Species Human
Gene Type protein-coding
Description interleukin-1 receptor-associated kinase 1
Other Description IRAK-1|Pelle homolog
Swissprots Q96C06 D3DWW4 D3DWW3 Q96RL2 P51617 Q7Z5V4
Accessions AAC08756 CCQ43144 EAW72760 EAW72761 EAW72762 EAW72763 EAW72764 EAW72765 P51617 AF346607 AAK62888 AM392624 CAL37502 AM393211 CAL38089 BC014963 AAH14963 BC054000 AAH54000 BE276817 DQ054788 AAY88246 EU831427 ACE87114 EU831520 ACE86439 L76191 AAC41949 XM_005274668 XP_005274725 XM_011531158 XP_011529460 NM_001025242 NP_001020413 NM_001025243 NP_001020414 NM_001569 NP_001560
Function Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor- signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation a
Subcellular Location Cytoplasm {ECO:0000269|PubMed:16690127}. Nucleus {ECO:0000269|PubMed:16690127}. Lipid droplet {ECO:0000250}. Note=Translocates to the nucleus when sumoylated. RSAD2/viperin recruits it to the lipid droplet (By similarity). {ECO:0000250}.
Tissue Specificity Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. {ECO:0000269|PubMed:11397809}.
Top Pathways Toxoplasmosis, Epstein-Barr virus infection, Tuberculosis, Measles, Pertussis

IRAK1 (D51G7) Rabbit mAb (Biotinylated) - 11123 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D51G7
Western blot analysis of extracts from various cell lines using IRAK1 (D51G7) Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for detection.

IRAK1 (D51G7) Rabbit mAb - 4504 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D51G7
Western blot analysis of extracts from RD, 293 and A20 cells using IRAK1 (D51G7) Rabbit mAb.

IRAK1 Antibody (Human Specific) - 4359 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, 293, and MCF7 cells using IRAK1 (Human Specific) Antibody.

Phospho-IRAK1 (Thr209) Antibody - 12756 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-IRAK1 (Thr209) Antibody - 4986 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-IRAK1 (Thr387) Antibody - 4365 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-IRAK1 (Ser376) Antibody - 4361 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

IRAK1 Antibody - 4362 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines, using IRAK1 Antibody.

p-IRAK-1 (Ser 376) - sc-325147 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

IRAK-1 (B-5) - sc-55530 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IRAK-1 (B-5): sc-55530. Western blot analysis of IRAK-1 expression in HeLa (A) and PMA treated K-562 (B) whole cell lysates and K-562 (C) and PMA treated K-562 (D) nuclear extracts.

IRAK-1 (C-19) - sc-1894 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
IRAK-1 (C-19): sc-1894. Western blot analysis of human recombinant IRAK-1 fusion protein.

IRAK-1 (C-2) - sc-5287 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IRAK-1 (C-2): sc-5287. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic and membrane staining of glandular cells and membrane staining of adipocytes.

IRAK-1 (F-4) - sc-5288 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
IRAK-1 (F-4): sc-5288. Western blot analysis of IRAK-1 expression in K-562 (A) and 3611-RF + PMA (B) nuclear extracts.

IRAK-1 (H-273) - sc-7883 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
IRAK-1 (H-273): sc-7883. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing membrane and cytoplasmic staining.

Anti-IRAK (phospho T100) antibody - ab63484 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This image shows human colon carcinoma tissue stained with ab63484 at 1/50 dilution; with and without the immunising phosphopeptide.

Anti-IRAK (phospho T387) antibody - ab139739 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IRAK (phospho T387) antibody (ab139739) at 1 µg/mlLane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell LysateLane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate with Human IRAK (phospho T387) peptide (ab174118) at 1 µg/mlLane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate with Human IRAK peptide (ab174119) at 1 µg/mlLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-IRAK (phospho T387) antibody - ab112042 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab112042 at 1/100 dilution staining IRAK in NIH3T3 cells by Immunofluorescence. The image on the right is treated with the synthesized phosphopeptide.

Anti-IRAK antibody - ab238 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IRAK antibody (ab238) at 1/2000 dilutionLane 1 : THP-1 (THP)whole cell lysateLane 2 : HeLa (HL) whole cell lysate

Anti-IRAK antibody - ab47843 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-IRAK antibody (ab47843) at 1/2000 dilutionLane 1 : THP whole cell lysateLane 2 : HeLa whole cell lysateLysates/proteins at 15 µg per lane.SecondaryHRP conjugated anti-rabbit IgG at a dilution of 1:10000

Anti-IRAK antibody - ab5522 from Abcam

Prices $379.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

Anti-IRAK antibody - ab55316 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
IRAK antibody (ab55316) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.

Anti-IRAK antibody - ab55318 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
IRAK antibody (ab55318) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lung.

Anti-IRAK antibody - ab62700 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab62700, at 1/50 dilution, staining IRAK in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.

Anti-IRAK antibody - ab59220 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-IRAK antibody (ab59220) at 1/500 dilutionLane 1 : Jurkat cell extractLane 2 : Jurkat cell extract with immunising peptide

Anti-IRAK antibody [3F7] - ab119289 from Abcam

Prices $384.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 3F7
ab119289 at 5 µg/ml staining IRAK in Formalin-Fixed, Paraffin-Embedded Human Placenta tissue by Immunohistochemistry.
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