CHAF1B

Gene Symbol CHAF1B
Entrez Gene 8208
Alt Symbol CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7
Species Human
Gene Type protein-coding
Description chromatin assembly factor 1, subunit B (p60)
Other Description CAF-1 subunit B|CAF-I 60 kDa subunit|CAF-I p60|M-phase phosphoprotein 7|chromatin assembly factor 1 subunit B|chromatin assembly factor I p60 subunit|human chromatin assembly factor-I p60 subunit
Swissprots Q13112 Q99548
Accessions BAA89426 BAA95549 EAX09741 Q13112 AJ001856 AK092781 AK313152 BAG35970 AL711314 BC001143 BC021218 AAH21218 JF432525 ADZ15742 U20980 AAA76737 X98262 CAA66915 XM_011529753 XP_011528055 XM_011529754 XP_011528056 XM_011529755 XP_011528057 NM_005441 NP_005432
Function Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. {ECO:0000269|PubMed:9813080}.
Subcellular Location Nucleus {ECO:0000269|PubMed:9614144}. Cytoplasm {ECO:0000269|PubMed:9614144}. Note=DNA replication foci. Cytoplasmic in M phase.

CAF-1 p60 (B-10) - sc-393662 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAF-1 p60 (B-10): sc-393662. Western blot analysis of CAF-1 p60 expression in HeLa (A) and Jurkat (B) whole cell lysates.

CAF-1 p60 (SS 53) - sc-56646 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAF-1 p60 (SS 53): sc-56646. Western blot analysis of CAF-1 p60 expression in HeLa (A) and Jurkat (B) nuclear extracts.

CAF-1 p60 (C-16) - sc-10982 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CAF-1 p60 (C-16): sc-10982. Western blot analysis of CAF-1 p60 expression in HeLa (A) whole cell lysate and Jurkat (B) nuclear extract.

CAF-1 p60 (H-80) - sc-367905 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CAF-1 p60 (H-80): sc-367905. Western blot analysis of CAF-1 p60 expression in mouse testis tissue extract.

Anti-p60 CAF1 antibody - ab72520 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling p60 CAF1 with ab7252 at 1/200 (1µg/ml). Detection: DAB.

Anti-p60 CAF1 antibody - ab168648 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-p60 CAF1 antibody (ab168648) at 1 µg/mlLane 1 : p60 CAF1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysate Lysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-p60 CAF1 antibody - ab72519 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation/ Western Blot of p60 CAF1. Lane 1: ab72519 at 3µg/mg Hela whole cell lysate (at 1mg for IP, 20% of IP loaded) Lane 2: Control IgG. A different antibody was used for the WB.

Anti-p60 CAF1 antibody [EPR6105] - ab109442 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6105
Overlay histogram showing HeLa cells stained with ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109442, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-p60 CAF1 antibody [SS 53] - ab8133 from Abcam

Prices $380.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone SS 53
ab8133 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/800 and incubated with the sample for 1 hour at 20°C.  A Cy3® conjugated donkey anti-mouse antibody diluted 1/400 was used as the secondary.See Abreview

Anti-CHAF1B / CAF1 Antibody (clone p60-24) - LS-C179638 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone p60-24

Anti-CHAF1B / CAF1 Antibody (C-Terminus) - LS-C80777 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
CHAF1B antibody LS-C80777 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.

Anti-CHAF1B / CAF1 Antibody (C-Terminus) - LS-C287916 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human CAF-1 p60 by Immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:200 (1 ug/ml).

Anti-CHAF1B / CAF1 Antibody (C-Terminus) - LS-C286473 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human CAF-1 p60 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:250.

Anti-CHAF1B / CAF1 Antibody - LS-C335645 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of MCF-7 cell using CHAF1B antibody. Blue: DAPI for nuclear staining.

Anti-CHAF1B / CAF1 Antibody (C-Terminus, clone EPR6105) - LS-C138618 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6105
IHC of paraffin-embedded human tonsil tissue using anti-CAF-1 p60 antibody.

Anti-CHAF1B / CAF1 Antibody (clone 4F7) - LS-C338213 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4F7
IHC of paraffin-embedded Human lymph node tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

Anti-CHAF1B / CAF1 Antibody (C-Terminus) - LS-C79303 from LifeSpan Bioscience

Prices $545.00
Sizes 20 µg
Host Rabbit
Clonality Polyclonal

Anti-CHAF1B / CAF1 Antibody (aa375-425) - LS-C287914 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-CHAF1B / CAF1 Antibody (clone SS 53) - LS-C2627 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
CAF-1 p60 (SS53) Antibody - Western Blot of: A. RKO cells with p150 ab, PCNA ab, CHAF1B Antibody LS-C2627 B. p150 RNAi knockdown

Anti-CHAF1B / CAF1 Antibody (N-Terminus) - LS-C111068 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CHAF1B antibody LS-C111068 Western blot of Fetal Liver lysate.

Anti-CHAF1B / CAF1 Antibody (aa44-93) - LS-C200195 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from RAW264.7cells using CAF1B antibody.

Anti-CHAF1B / CAF1 Antibody (aa71-120) - LS-C119007 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from Jurkat cells, using CAF1B Antibody. The lane on the right is treated with the synthesized peptide.

Anti-CHAF1B / CAF1 Antibody (clone SS 24 1-68) - LS-C2626 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
CAF-1 p60 Antibody (SS 24 1-68) - CAF-1 p60 (SS24 1-68) - Immunocytochemical staining of CAF-1 p60 in HeLa cells (Smith and Stillman 1991).

Anti-CHAF1B / CAF1 Antibody (clone 2G7) - LS-C198536 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2G7
Detection limit for recombinant GST tagged CHAF1B is 0.1 ng/ml as a capture antibody.

Anti-CHAF1B / CAF1 Antibody (Trp93) - LS-C177124 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of CAF-1 p60 (W93) pAb in extracts from Jurkat cells.
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