SMAD7

Gene Symbol SMAD7
Entrez Gene 4092
Alt Symbol CRCS3, MADH7, MADH8
Species Human
Gene Type protein-coding
Description SMAD family member 7
Other Description MAD (mothers against decapentaplegic, Drosophila) homolog 7|MAD homolog 8|Mothers against decapentaplegic, drosophila, homolog of, 7|SMAD, mothers against DPP homolog 7|hSMAD7|mothers against DPP homolog 8|mothers against decapentaplegic homolog 7
Swissprots Q6DK23 B7Z773 K7EQ10 O14740 O15105
Accessions AAL68977 EAW62931 O15105 AF010193 AAB81246 AF015261 AAB81354 AK131394 BAG54770 AK301535 BAH13509 BC074818 AAH74818 BC074819 AAH74819 BP363832 DA882147 NM_001190821 NP_001177750 NM_001190822 NP_001177751 NM_001190823 NP_001177752 NM_005904 NP_005895
Function Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A- PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity). {ECO:0000250}.
Subcellular Location Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 or induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta.
Tissue Specificity Ubiquitous with higher expression in the lung and vascular endothelium.
Top Pathways TGF-beta signaling pathway, Endocytosis, Hippo signaling pathway

Smad6/7 (N-19) - sc-7004 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad6/7 (N-19): sc-7004. Western blot analysis of non-transfected (A) and Smad7-transfected (B) COS cells.

Smad7 (B-8) - sc-365846 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad7 (B-8): sc-365846. Western blot analysis of Smad7 expression in A549 whole cell lysate.

Smad7 (H-79) - sc-11392 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Smad7 (H-79): sc-11392. Immunofluorescence staining of methanol-fixed A549 cells showing perinuclear localization.

Smad7 (P-20) - sc-9183 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Smad7 (Z8-B) - sc-101152 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Smad7 (Z8-B): sc-101152. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human placenta tissue showing nuclear localization.

Smad7 (G-23) - sc-134013 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Smad7 (G-23): sc-134013. Western blot analysis of Smad7 expression in Jurkat whole cell lysate.

Anti-MADH7 antibody - ab76498 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-MADH7 antibody (ab76498) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab52255)Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770)Lysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG VHH Single Domain Antibody (HRP) (ab191866) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MADH7 antibody - ab90086 from Abcam

Prices $378.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-MADH7 antibody (ab90086) at 1/500 dilution + HeLa cell lysate

Anti-MADH7 antibody - ab55493 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing A549 cells stained with ab55493 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55493, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-MADH7 antibody [EPR622] - ab124890 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR622
All lanes : Anti-MADH7 antibody [EPR622] (ab124890) at 1/1000 dilutionLane 1 : Human Uterus lysateLane 2 : Human Ovary Cancer lysateLane 3 : Human Fetal Lung lysateLane 4 : Human Skeletal Muscle lysateLane 5 : Human Kidney lysateLane 6 : Human Heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-MADH7 antibody - C-terminal - ab190987 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin fixed paraffin embedded rat brain labeling MADH7 with ab190987 at 1 µg/ml.

Anti-SMAD7 Antibody (aa12-18) - LS-C10232 from LifeSpan Bioscience

Prices $385.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD7 Antibody - LS-C48612 from LifeSpan Bioscience

Prices $410.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMAD7 Antibody (aa320-398) - LS-C39135 from LifeSpan Bioscience

Prices $530.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SMAD7 Antibody (aa12-29, Azide-free) - LS-C147986 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMAD7 Antibody (Internal) - LS-C137159 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD7 Antibody (aa230-426) - LS-C311805 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD7 Antibody - LS-C341084 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMAD7 Antibody (aa24-73) - LS-C29767 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal Lung Lysate

Anti-SMAD7 Antibody (N-Terminus) - LS-C79985 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane A: Marker. Lane B: Jurkat cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.

Anti-SMAD7 Antibody (Internal, clone EPR622) - LS-C138661 from LifeSpan Bioscience

Prices $480.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR622
Western blot analysis on (A) uterus, (B) ovary cancer, (C) fetal lung, (D) human skeletal muscle, (E) human kidney, and (F) human heart lysates using anti-Smad7 antibody.

Anti-SMAD7 Antibody (aa375-389) - LS-C313088 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
SMAD7 antibody. IHC(P): Rat Brain Tissue.

Anti-SMAD7 Antibody (aa36-50) IHC-plus™ - LS-B120 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SMAD7 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B120 concentration 10 ug/ml.

Anti-SMAD7 Antibody (clone 293039) - LS-C150142 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 293039
Smad7 in Human Liver Cancer Tissue. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 ug/ml Human/Mouse/Rat Smad7 Monoclonal Antibody overnight at 4°C. Tissue was stained with Anti-Mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).

Anti-SMAD7 Antibody (aa195-224) - LS-C161302 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Smad7 Antibody IHC of formalin-fixed and paraffin-embedded lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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