PGAM5

Gene Symbol PGAM5
Entrez Gene 192111
Alt Symbol BXLBV68
Species Human
Gene Type protein-coding
Description phosphoglycerate mutase family member 5
Other Description Bcl-XL-binding protein v68|serine/threonine-protein phosphatase PGAM5, mitochondrial
Swissprots A9LN06 C9IZY7 Q96JB0 Q96HS1
Accessions EAW54825 EAW54826 EAW54827 Q96HS1 AF357523 AAK60627 AK097688 BC008196 AAH08196 BC047906 CA307621 CR995429 D25830 DR155760 EU249757 ABX39494 NM_001170543 NP_001164014 NM_001170544 NP_001164015 NM_138575 NP_612642
Function Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2- dependent gene expression. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore. {ECO:0000269|PubMed:18387606, ECO:0000269|PubMed:19590015, ECO:0000269|PubMed:22265414}.
Subcellular Location Mitochondrion outer membrane {ECO:0000269|PubMed:18387606}; Single-pass membrane protein {ECO:0000269|PubMed:18387606}. Note=Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus.
Top Pathways TNF signaling pathway

PGAM5 (K-16) - sc-161156 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PGAM5 (K-16): sc-161156. Western blot analysis of PGAM5 expression in MCF7 whole cell lysate.

PGAM5 (E-13) - sc-161155 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PGAM5 (T-15) - sc-161157 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PGAM5 (T-15): sc-161157. Western blot analysis of PGAM5 expression in K-562 (A), MOLT-4 (B) and RT-4 (C) whole cell lysates.

Anti-PGAM5 antibody - ab126534 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent staining of Human cell line MCF-7 shows positivity in mitochondria. Recommended concentration of ab126534 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Anti-PGAM5 antibody - ab131552 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab131552 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab131552 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PGAM5 antibody - ab126505 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PGAM5 antibody (ab126505) at 1/250 dilutionLane 1 : RT 4 cell lysateLane 2 : U 251 MG cell lysateLane 3 : Human plasmaLane 4 : Human liver tissue lysateLane 5 : Human tonsil tissue lysate

PGAM5 Antibody - NBP1-92257 from Novus Biologicals

Prices $129.00, $419.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PGAM5 Antibody [NBP1-92257] - Analysis in human cell line A-549.

PGAM5 Antibody - NBP1-92258 from Novus Biologicals

Prices $129.00, $419.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal

Monoclonal Anti-PGAM5 antibody produced in mouse - AMAB90803 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone CL0624
<B>Immunohistochemistry</B><BR/>Monoclonal Anti-PGAM5 <B>Cat. No. AMAB90803</B>: Immunohistochemical staining of human kidney shows either strong or moderate cytoplasmic immunoreactivity in subsets of renal tubules.

Anti-PGAM5 (186-200) antibody produced in rabbit - SAB1103344 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human PGAM5 was separated on SDS-PAGE, blotted with Anti-PGAM5 (186-200) (<B>Cat. No. SAB1103344</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000<BR/>2. Antibody dilution 1:1,000 + PGAM5 immunizing peptide (human, 186-200)

Anti-PGAM5 antibody produced in rabbit - HPA036978 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PGAM5: <B>Cat. No. HPA036978</B>: Immunohistochemical staining of human uterus, pre-menopause shows strong cytoplasmic positivity in glandular cells.

Anti-PGAM5 polyclonal antibody - DPABH-22857 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Rabbit anti-Human PGAM5 polyclonal antibody - DPABH-22887 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Anti-PGAM5 (aa 100-200) polyclonal antibody - DPABH-25192 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Anti-PGAM5 Antibody - ABC517 from EMD Millipore

Prices $319.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blotting Analysis:<br />Representative lot data.<br />HT-29 cell lysate was probed with Cat. No. ABC517, Anti-PGAM5 (1.0 µg/mL). <br />Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.<br />Arrows indicate PGAM5 (~32/34 kDa)

PGAM5 antibody - GTX119887 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-PGAM5 antibody used in Western blot. GTX119887

Anti-PGAM5 antibody produced in rabbit - HPA036979 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PGAM5: <B>Cat. No. HPA036979</B>: Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in granular pattern in exocrine pancreas.

PGAM5 antibody (FITC) - orb190230 from Biorbyt

Prices $315.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal

PGAM5 antibody - orb185916 from Biorbyt

Prices $255.00, $340.00
Sizes 100 μg, 200 μg
Host Rabbit
Clonality Polyclonal

Anti-PGAM5 Antibody - ABC517 from Sigma-Aldrich

Prices $319.00
Sizes abc517
Clonality Polyclonal
Western Blotting Analysis:<br />Representative lot data.<br />HT-29 cell lysate was probed with Cat. No. ABC517, Anti-PGAM5 (1.0 µg/mL). <br />Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.<br />Arrows indicate PGAM5 (~32/34 kDa)

ANTI-PGAM5 (C-TERM) antibody produced in rabbit - SAB1304113 from Sigma-Aldrich

Prices $336.50
Sizes 400 µl
Clonality Polyclonal

PGAM5 Antibody (CL0624) - NBP2-52947 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone CL0624
Western Blot: PGAM5 Antibody (CL0624) [NBP2-52947] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4

PGAM5, Polyclonal Antibody - MBS7050831 from MyBioSource

Prices $160.00, $235.00
Sizes 50 µg, 100 µg
Host Rabbit
Clonality Polyclonal

PGAM5, Polyclonal Antibody - MBS7050832 from MyBioSource

Prices $160.00, $235.00
Sizes 50 µg, 100 µg
Host Rabbit
Clonality Polyclonal

PGAM5, Polyclonal Antibody - MBS7050833 from MyBioSource

Prices $160.00, $235.00
Sizes 50 µg, 100 µg
Host Rabbit
Clonality Polyclonal
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