DDX25

Gene Symbol	DDX25
Entrez Gene	29118
Alt Symbol	GRTH
Species	Human
Gene Type	protein-coding
Description	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Other Description	ATP-dependent RNA helicase DDX25\|DEAD (Asp-Glu-Ala-Asp) box polypeptide 25\|DEAD box protein 25\|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25\|gonadotropin-regulated testicular RNA helicase
Swissprots	Q86W81 Q5XVN2 Q9UHL0 Q8IYP1 B2R6Z0
Accessions	AAU84667 ABW87006 ABW87007 ACA64098 EAW67668 EAW67669 Q9UHL0 AF155140 AAF21371 AK295121 BAG58148 AK312772 BAG35637 BC031098 BC035388 AAH35388 BC050360 AAH50360 CD359894 CD657404 DC395868 XM_011542790 XP_011541092 XM_011542791 XP_011541093 NM_013264 NP_037396
Function	ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity). {ECO:0000250}.
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:10608860}. Nucleus {ECO:0000250}. Note=Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids (By similarity). {ECO:0000250}.
Tissue Specificity	Highly expressed in the Leydig and germ cells of the testis and weakly expressed in the pituitary and hypothalamus. {ECO:0000269\|PubMed:10608860, ECO:0000269\|Ref.2}.

DDX25 (F-10) - sc-271730 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

DDX25 (F-10): sc-271730. Western blot analysis of DDX25 expression in F9 (A), IMR-32 (B) and NTERA-2 cl.D1 (C) whole cell lysates.

DDX25 (G-4) - sc-166289 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

DDX25 (G-4): sc-166289. Western blot analysis of DDX25 expression in non-transfected: sc-117752 (A) and human DDX25 transfected: sc-112550 (B) 293T whole cell lysates.

DDX25 (C-15) - sc-51269 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

DDX25 (C-15): sc-51269. Western blot analysis of DDX25 expression in non-transfected: sc-117752 (A) and human DDX25 transfected: sc-112550 (B) 293T whole cell lysates.

DDX25 (N-20) - sc-51271 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

DDX25 (N-20): sc-51271. Western blot analysis of DDX25 expression in rat testis (A) and mouse testis (B) tissue extracts.

DDX25 (H-40) - sc-134871 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

DDX25 (H-40): sc-134871. Western blot analysis of DDX25 expression in mouse brain tissue extract.

Anti-DDX25 antibody - ab49340 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Lane 1 : MarkerLane 2 : Anti-DDX25 antibody (ab49340) at 0.5 µg/mlLane 1 : As aboveLane 2 : Jurkat cell lysate at 10 µgSecondaryLane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-DDX25 Antibody (aa355-404) - LS-C30163 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-DDX25 Antibody (aa180-229) - LS-C110154 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

DDX25 antibody LS-C110154 Western blot of HepG2 cell lysate.

DDX25 Antibody - NBP1-57341 from Novus Biologicals

Prices	$139.00, $329.00
Sizes	20 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal

DDX25 Antibody - NBP1-83329 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry-Paraffin: DDX25 Antibody [NBP1-83329] - Staining of human rectum shows strong nuclear positivity in glandular cells.

DDX25 Antibody - NBP1-83328 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: DDX25 Antibody [NBP1-83328] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-153

Anti-DDX25 antibody produced in rabbit - HPA020090 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-DDX25: <B>Cat. No. HPA020090</B>: Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.

Anti-DDX25 antibody produced in rabbit - HPA020137 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-DDX25: <B>Cat. No. HPA020137</B>: Immunohistochemical staining of human testis shows strong positivity in seminiferus duct cells.

Anti-DDX25 antibody produced in rabbit - AV36410 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell Type: Jurkat (<B>Cat. No. AV36410</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 0.5 μg/mL in cell type Jurkat

Anti-DDX25 antibody produced in rabbit - SAB2100551 from Sigma-Aldrich

Prices	$347.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-DDX25: <B>Cat. No.SAB2100551</B>: DDX25 in HepG2 cell lysate with DDX25 antibody at 1.0 μg/mL.

DDX25 antibody - middle region (ARP36409_P050) - ARP36409_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

DDX25 antibody - C-terminal region (ARP36410_P050) - ARP36410_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

DDX25 antibody, C-term - GTX47517 from GeneTex

Prices	$319.00
Sizes	50 μg
Host	Rabbit
Clonality	Polyclonal

Anti-DDX25 antibody, C-term used in Western blot. GTX47517

DDX25 antibody - 70R-4792 from Fitzgerald

Prices	$345.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

DDX25 antibody - 70R-16785 from Fitzgerald

Prices	$375.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

DDX25 antibody - 70R-4822 from Fitzgerald

Prices	$345.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

DDX25 antibody - 14370-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Human testis were subjected to SDS PAGE followed by western blot with 14370-1-AP(DDX25 Antibody) at dilution of 1:800

DDX25 Antibody - 28-491 from ProSci

Prices	$315.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Antibody used in WB on Human Jurkat 0.2-1 ug/ml.

DDX25 antibody - 14370-1-AP from Proteintech Group

Prices	$279.00
Sizes	con: 20 μg/150μl
Host	Rabbit
Clonality	Polyclonal

mouse testis tissue were subjected to SDS PAGE followed by western blot with 14370-1-AP(DDX25 antibody) at dilution of 1:500

DDX25 Antibody - GWB-MM382D from Genway Biotech

Prices	$404.00
Sizes	50 µg
Clonality	Polyclonal

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