AGAP2

Gene Symbol AGAP2
Entrez Gene 116986
Alt Symbol CENTG1, GGAP2, PIKE
Species Human
Gene Type protein-coding
Description ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Other Description Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2|GTP-binding and GTPase activating protein 2|arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2|centaurin, gamma 1|cnt-g1|phosphatidylinositol 3-kinase enhancer|phosphatidylinositol-3-kinase enhancer|phosphoinositide 3-kinase enhancer
Swissprots Q8IWU3 Q99490 A8K9F7 Q548E0 O00578
Accessions AAC39522 EAW97048 EAW97049 EAW97050 EAW97051 Q99490 AF384128 AAO39848 AF413077 AAL04171 AK122827 BAG53747 AK292672 BAF85361 AV740795 AY128689 AAM97540 BC028020 AAH28020 BE280072 BI755875 D79989 BAA11484 DB165417 XM_005268625 XP_005268682 XM_005268626 XP_005268683 XR_245891 NM_001122772 NP_001116244 NM_014770 NP_055585
Function GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. {ECO:0000269|PubMed:12640130, ECO:0000269|PubMed:14761976, ECO:0000269|PubMed:15118108, ECO:0000269|PubMed:16079295}.
Subcellular Location Isoform 1: Cytoplasm. Nucleus.
Tissue Specificity Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart. {ECO:0000269|PubMed:12640130, ECO:0000269|PubMed:14761976, ECO:0000269|PubMed:15118108, ECO:0000269|PubMed:16079295, ECO:0000269|PubMed:16150119}.
Top Pathways Endocytosis

PIKE (G-9) - sc-166864 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PIKE (G-9): sc-166864. Western blot analysis of PIKE expression in non-transfected: sc-117752 (A) and human PIKE transfected: sc-114780 (B) 293T whole cell lysates.

PIKE (D-19) - sc-55372 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PIKE (D-19): sc-55372. Western blot analysis of PIKE expression in non-transfected: sc-117752 (A) and human PIKE transfected: sc-114780 (B) 293T whole cell lysates.

PIKE (P-18) - sc-55376 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PIKE (P-18): sc-55376. Western blot analysis of PIKE expression in non-transfected: sc-117752 (A) and human PIKE transfected: sc-114780 (B) 293T whole cell lysates.

Anti-Centaurin gamma 1 antibody - ab105332 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Centaurin gamma 1 antibody (ab105332) at 1/500 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal liver lysateLane 3 : Human fetal heart lysate

Anti-Centaurin gamma 1 antibody - ab21271 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-Centaurin gamma 1 antibody (ab21271) at 0.5 µg/mlLane 2 : Anti-Centaurin gamma 1 antibody (ab21271) at 1 µg/mlLane 3 : Anti-Centaurin gamma 1 antibody (ab21271) at 2 µg/mlLane 1 : Mouse brain cell lysateLane 2 : Mouse brain cell lysateLane 3 : Mouse brain cell lysate

Anti-Centaurin gamma 1 antibody - ab51796 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Centaurin gamma 1 antibody (ab51796) at 1/10000 dilutionLane 1 : HEK293 cell lysate (empty vector)Lane 2 : HEK293 cell lysate transfected with Centaurin gamma 1 recombinant protein

Anti-Centaurin gamma 1 antibody - ab87643 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Centaurin gamma 1 antibody (ab87643) at 0.3 µg/ml + Human amygdala lysate at 35 µgdeveloped using the ECL technique

Anti-Centaurin gamma 1 antibody - ab110956 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (C-Terminus) - LS-C121282 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (Internal) - LS-C145163 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (aa719-768) - LS-C116484 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
AGAP2 / PIKE antibody LS-C116484 Western blot of THP-1 cell lysate.

Anti-AGAP2 / PIKE Antibody (aa850-877, AP) - LS-C254626 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (aa850-877, APC) - LS-C254627 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (aa850-877) - LS-C168676 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
CENTG1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The CENTG1 antibody detected the CENTG1 protein (arrow).

Anti-AGAP2 / PIKE Antibody (aa850-877, Biotin) - LS-C254628 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (aa850-877, FITC) - LS-C254629 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (aa850-877, Azide-free, HRP) - LS-C254630 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (aa850-877, PE) - LS-C254633 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (aa700-750) - LS-C290906 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (aa850-877) - LS-C320326 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AGAP2 / PIKE Antibody (aa450-500) - LS-C290904 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PIKE by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PIKE antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-AGAP2 / PIKE Antibody (aa20-60) - LS-C148542 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC ofAGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148542 (bottom left, right) at 5 ug/ml.

Anti-AGAP2 / PIKE Antibody (C-Terminus) IHC-plus™ - LS-B134 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.

Anti-AGAP2 / PIKE Antibody (aa1-836) IHC-plus™ - LS-B338 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.

Anti-AGAP2 / PIKE Antibody (aa450-500) - LS-C148580 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC ofAGAP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and LS-C148580 (bottom left, right) at 5 ug/ml.
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