AGAP2
| Gene Symbol | AGAP2 |
|---|---|
| Entrez Gene | 116986 |
| Alt Symbol | CENTG1, GGAP2, PIKE |
| Species | Human |
| Gene Type | protein-coding |
| Description | ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 |
| Other Description | Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2|GTP-binding and GTPase activating protein 2|arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2|centaurin, gamma 1|cnt-g1|phosphatidylinositol 3-kinase enhancer|phosphatidylinositol-3-kinase enhancer|phosphoinositide 3-kinase enhancer |
| Swissprots | Q8IWU3 Q99490 A8K9F7 Q548E0 O00578 |
| Accessions | AAC39522 EAW97048 EAW97049 EAW97050 EAW97051 Q99490 AF384128 AAO39848 AF413077 AAL04171 AK122827 BAG53747 AK292672 BAF85361 AV740795 AY128689 AAM97540 BC028020 AAH28020 BE280072 BI755875 D79989 BAA11484 DB165417 XM_005268625 XP_005268682 XM_005268626 XP_005268683 XR_245891 NM_001122772 NP_001116244 NM_014770 NP_055585 |
| Function | GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. {ECO:0000269|PubMed:12640130, ECO:0000269|PubMed:14761976, ECO:0000269|PubMed:15118108, ECO:0000269|PubMed:16079295}. |
| Subcellular Location | Isoform 1: Cytoplasm. Nucleus. |
| Tissue Specificity | Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart. {ECO:0000269|PubMed:12640130, ECO:0000269|PubMed:14761976, ECO:0000269|PubMed:15118108, ECO:0000269|PubMed:16079295, ECO:0000269|PubMed:16150119}. |
| Top Pathways | Endocytosis |
PIKE CRISPR/Cas9 KO Plasmid (h) - sc-405344 from Santa Cruz Biotechnology
|
||||||||
PIKE HDR Plasmid (h) - sc-405344-HDR from Santa Cruz Biotechnology
|
||||||||
PIKE Double Nickase Plasmid (h) - sc-405344-NIC from Santa Cruz Biotechnology
|
||||||||
PIKE Double Nickase Plasmid (h2) - sc-405344-NIC-2 from Santa Cruz Biotechnology
|
||||||||
PIKE CRISPR Activation Plasmid (h) - sc-405344-ACT from Santa Cruz Biotechnology
|
||||||||
PIKE CRISPR Activation Plasmid (h2) - sc-405344-ACT-2 from Santa Cruz Biotechnology
|
||||||||
PIKE Lentiviral Activation Particles (h) - sc-405344-LAC from Santa Cruz Biotechnology
|
||||||||
PIKE Lentiviral Activation Particles (h2) - sc-405344-LAC-2 from Santa Cruz Biotechnology
|
||||||||
PIKE siRNA (h) - sc-62812 from Santa Cruz Biotechnology
|
||||||||
PIKE shRNA Plasmid (h) - sc-62812-SH from Santa Cruz Biotechnology
|
||||||||
PIKE shRNA (h) Lentiviral Particles - sc-62812-V from Santa Cruz Biotechnology
|
||||||||
AGAP2 RNAi - H00116986-R03 from Novus Biologicals
|
||||||||
MISSION® esiRNA - EHU098821 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN2928 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND2928 from Sigma-Aldrich
|
||||||||
AGAP2 siRNA (Human) - CRJ4544 from Cohesion Biosciences
|
||||||||
AGAP2 - MBS8213851 from MyBioSource
|
||||||||
PIKE CRISPR/Cas9 KO Plasmid (h2) - sc-405344-KO-2 from Santa Cruz Biotechnology
|
||||||||
PIKE HDR Plasmid (h2) - sc-405344-HDR-2 from Santa Cruz Biotechnology
|
||||||||
shRNA set against Human AGAP2 (NM_014770.3) - SHH233126 from Creative biogene
|
||||||||
Human AGAP2 siRNA - orb275045 from Biorbyt
|
||||||||
AGAP2 shRNA Plasmid (Human) - CHJ4544 from Cohesion Biosciences
|
||||||||
AGAP2 miRNA 3'UTR clone - MiUTR3H-07714 from Creative biogene
|
||||||||
AGAP2 miRNA 3'UTR clone - MiUTR3H-07715 from Creative biogene
|