PIAS3

Gene Symbol PIAS3
Entrez Gene 10401
Alt Symbol ZMIZ5
Species Human
Gene Type protein-coding
Description protein inhibitor of activated STAT, 3
Other Description E3 SUMO-protein ligase PIAS3|protein inhibitor of activated STAT protein 3|zinc finger, MIZ-type containing 5
Swissprots Q9UFI3 Q9Y6X2
Accessions EAW71429 EAW71430 EAW71431 Q9Y6X2 AB021868 BAA78533 AK027557 BAG51345 AK125158 AK294231 BAG57533 AK304039 BAG64950 AL122061 CAB59241 AL133016 CAB61357 BC001154 AAH01154 BC030556 AAH30556 BE247489 BT007036 AAP35684 CR457090 CAG33371 DQ893542 ABM84468 DQ893796 ABM84722 DQ895037 ABM85963 EU176644 ABW03445 NM_006099 NP_006090
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. {ECO:0000269|PubMed:21965678, ECO:0000269|PubMed:9388184}.
Subcellular Location Cytoplasm. Nucleus. Note=Mainly cytoplasmic but shuttles between the nucleus and cytoplasm. Transported to the nucleus on interaction with phosphorylated STAT3. Colocalizes with GFI1 in nuclear dots (By similarity). {ECO:0000250}.
Tissue Specificity Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. {ECO:0000269|PubMed:9388184}.
Top Pathways Ubiquitin mediated proteolysis, Jak-STAT signaling pathway

SignalSilence ® PIAS3 siRNA II - 9031 from Cell Signaling Technology

Category siRNA
Prices $249.00
Sizes 300 µl (3 nmol)
Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® PIAS3 siRNA I #9073 (+), or SignalSilence ® PIAS3 siRNA II (+), using PIAS3 (D5F9) XP ® Rabbit mAb #9042 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The PIAS3 (D5F9) XP ® Rabbit mAb confirms silencing of PIAS3 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

SignalSilence ® PIAS3 siRNA I - 9073 from Cell Signaling Technology

Category siRNA
Prices $249.00
Sizes 300 µl (3 nmol)
Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® PIAS3 siRNA I (+), or SignalSilence ® PIAS3 siRNA II #9031 (+), using PIAS3 (D5F9) XP ® Rabbit mAb #9042 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The PIAS3 (D5F9) XP ® Rabbit mAb confirms silencing of PIAS3 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

PIAS 3 CRISPR/Cas9 KO Plasmid (h) - sc-401406 from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

PIAS 3 HDR Plasmid (h) - sc-401406-HDR from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

PIAS 3 Double Nickase Plasmid (h) - sc-401406-NIC from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

PIAS 3 Double Nickase Plasmid (h2) - sc-401406-NIC-2 from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

PIAS 3 CRISPR Activation Plasmid (h) - sc-401406-ACT from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

PIAS 3 CRISPR Activation Plasmid (h2) - sc-401406-ACT-2 from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

PIAS 3 Lentiviral Activation Particles (h) - sc-401406-LAC from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

PIAS 3 Lentiviral Activation Particles (h2) - sc-401406-LAC-2 from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

PIAS 3 siRNA (h) - sc-37005 from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

PIAS 3 shRNA Plasmid (h) - sc-37005-SH from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

PIAS 3 shRNA (h) Lentiviral Particles - sc-37005-V from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

PIAS3 RNAi - H00010401-R01V from Novus Biologicals

Category siRNA
Prices $399.00
Sizes 20 nmol
Species Reactivities Human
Western Blot: PIAS3 RNAi [H00010401-R01V] - Western blot anAncell line, cotransfected with PIAS3 Validated Chimera RNAi or non-transfected control. Blot probed with H00010401-M03. GAPDH (36.1 kDa) used as loading control.

PIAS3 RNAi - H00010401-R01 from Novus Biologicals

Category siRNA
Prices $399.00
Sizes 20 nmol
Species Reactivities Human

MISSION® esiRNA - EHU050391 from Sigma-Aldrich

Category siRNA

CompoZr® Knockout ZFN Kit - CKOZFN16775 from Sigma-Aldrich

Category ZFN Kit
Prices $4,999.00
Sizes 1 kt

CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND16775 from Sigma-Aldrich

Category ZFN Kit
Prices $3,999.00
Sizes 1 kt

PIAS3 siRNA (Human) - CRH7050 from Cohesion Biosciences

Category siRNA
Prices $340.00, $510.00
Sizes 15 nmol, 30 nmol
Species Reactivities Human

shRNA set against Human PIAS3(NM_006099.3) - SHR113024 from Creative biogene

Category siRNA
Species Reactivities Human

PIAS3 shRNA Plasmid (Human) - CHH7050 from Cohesion Biosciences

Category siRNA
Prices $500.00, $750.00
Sizes 150 µg, 300 µg
Species Reactivities Human

PIAS3 miRNA 3'UTR clone - MiUTR1H-07882 from Creative biogene

Species Reactivities Human
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