ZAP70

Gene Symbol	ZAP70
Entrez Gene	7535
Alt Symbol	SRK, STCD, STD, TZK, ZAP-70
Species	Human
Gene Type	protein-coding
Description	zeta-chain (TCR) associated protein kinase 70kDa
Other Description	70 kDa zeta-associated protein|70 kDa zeta-chain associated protein|syk-related tyrosine kinase|tyrosine-protein kinase ZAP-70|zeta-chain associated protein kinase, 70kD
Swissprots	Q6PIA4 A6NFP4 Q9UBS6 P43403 Q8IXD6
Accessions	AAX93187 EAX01918 EAX01919 P43403 AB083211 BAC43747 AK303341 BAG64404 AK310628 AK310637 AL832287 BC039039 AAH39039 BC053878 AAH53878 L05148 XM_005264015 XP_005264072 XM_006712728 XP_006712791 XM_011511783 XP_011510085 XR_923018 XR_923019 XR_923020 NM_001079 NP_001070 NM_207519 NP_997402
Function	Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, ad
Subcellular Location	Cytoplasm {ECO:0000269|PubMed:9813084}. Cell membrane {ECO:0000269|PubMed:9813084}; Peripheral membrane protein {ECO:0000269|PubMed:9813084}. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Colocalizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity). {ECO:0000250}.
Tissue Specificity	Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells. {ECO:0000269|PubMed:1423621, ECO:0000269|PubMed:16467082, ECO:0000269|PubMed:9378960}.
Top Pathways	Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, Primary immunodeficiency

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

Zap-70 CRISPR/Cas9 KO Plasmid (h) - sc-400826 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

Zap-70 HDR Plasmid (h) - sc-400826-HDR from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

Zap-70 Double Nickase Plasmid (h) - sc-400826-NIC from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

Zap-70 Double Nickase Plasmid (h2) - sc-400826-NIC-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

Zap-70 CRISPR Activation Plasmid (h) - sc-400826-ACT from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

Zap-70 CRISPR Activation Plasmid (h2) - sc-400826-ACT-2 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

Zap-70 Lentiviral Activation Particles (h) - sc-400826-LAC from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

Zap-70 Lentiviral Activation Particles (h2) - sc-400826-LAC-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

ZAP-70 siRNA (h) - sc-29526 from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

ZAP-70 shRNA Plasmid (h) - sc-29526-SH from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

ZAP-70 shRNA (h) Lentiviral Particles - sc-29526-V from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

ZAP70 RNAi - H00007535-R03 from Novus Biologicals Category siRNA Prices $399.00 Sizes 20 nmol Species Reactivities Human

ZAP70 RNAi - H00007535-R04 from Novus Biologicals Category siRNA Prices $399.00 Sizes 20 nmol Species Reactivities Human

MISSION® esiRNA - EHU091301 from Sigma-Aldrich Category siRNA Prices $140.00, $250.00 Sizes 20 µg, 50 µg

MISSION® siRNA - SIHK2490 from Sigma-Aldrich Category siRNA Prices $25.00 Sizes 0.25 nmol

MISSION® siRNA - SIHK2488 from Sigma-Aldrich Category siRNA Prices $25.00 Sizes 0.25 nmol

MISSION® siRNA - SIHK2489 from Sigma-Aldrich Category siRNA Prices $25.00 Sizes 0.25 nmol

CompoZr® Knockout ZFN Kit - CKOZFN23043 from Sigma-Aldrich Category ZFN Kit Prices $4,999.00 Sizes 1 kt

CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND23043 from Sigma-Aldrich Category ZFN Kit Prices $3,999.00 Sizes 1 kt

MISSION® 3′UTR Lenti GoClone™ - HUTR03417 from Sigma-Aldrich Category miRNA Prices $350.00 Sizes hutr03417

ZAP70 siRNA (Human) - CRH5148 from Cohesion Biosciences Category siRNA Prices $340.00, $510.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

ZAP70 - MBS8230282 from MyBioSource Prices $365.00, $515.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

Zap-70 CRISPR/Cas9 KO Plasmid (h2) - sc-400826-KO-2 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

Zap-70 HDR Plasmid (h2) - sc-400826-HDR-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

shRNA set against Human ZAP70 (NM_001079.3) - SHH448796 from Creative biogene Category siRNA Species Reactivities Human

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