RRAGB

Gene Symbol	RRAGB
Entrez Gene	10325
Alt Symbol	RAGB, bA465E19.1
Species	Human
Gene Type	protein-coding
Description	Ras-related GTP binding B
Other Description	GTP-binding protein ragB\|rag B\|ras-related GTP-binding protein B
Swissprots	Q8N3T0 Q15348 Q5VZM2 A8K042
Accessions	EAW93227 EAW93228 EAW93229 Q5VZM2 AK289407 BAF82096 AL831926 CAD38586 BC034726 AAH34726 BX641797 CD676755 DB246060 DB252202 EU831588 ACE87188 EU831673 ACE86507 X90530 CAA62132 XM_005261973 XP_005262030 XM_005261975 XP_005262032 XR_244464 XR_244465 XR_430513 XR_938361 NM_006064 NP_006055 NM_016656 NP_057740
Function	Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. {ECO:0000269\|PubMed:18497260, ECO:0000269\|PubMed:20381137, ECO:0000269\|PubMed:9394008}.
Subcellular Location	Cytoplasm {ECO:0000269\|PubMed:7499430}. Lysosome.
Top Pathways	mTOR signaling pathway

RagB (D18F3) Rabbit mAb - 8150 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D18F3

Western blot analysis of extracts from various cell lines using RagB (D18F3) Rabbit mAb.

RagB Antibody - 3830 from Cell Signaling Technology

Host	Rabbit
Clonality	Polyclonal

Rag A/B (G-20) - sc-248345 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Rag A/B (C-18) - sc-248347 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Rag B (N-12) - sc-169101 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-RRAGA + RRAGB antibody - ab91062 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-RRAGA + RRAGB antibody (ab91062) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg/ml per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-RRAGA + RRAGB antibody - ab77911 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-RRAGA + RRAGB antibody (ab77911) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-RRAGB antibody - ab103671 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RRAGB antibody (ab103671) at 1/500 dilution + Human placenta tissue lysate at 50 µg

Anti-RRAGB antibody - ab172555 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-RRAGB antibody (ab172555) at 1 µg/mlLane 1 : RRAGB transfected 293T cell line lysateLane 2 : 293T cell line lysate (non-transfected)Lysates/proteins at 15 µl per lane.SecondaryGoat anti-mouse HRP conjugated antibody at 1/2500 dilutiondeveloped using the ECL technique

Anti-RRAGB antibody - ab56633 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Western blot against tagged recombinant protein immunogen using ab56633 RRAGB antibody at 1ug/ml.

Anti-RRAGB / RAGB Antibody - LS-C131949 from LifeSpan Bioscience

Prices	$445.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RRAGB / RAGB Antibody (aa1-30, AP) - LS-C268591 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RRAGB / RAGB Antibody (aa1-30, APC) - LS-C268592 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RRAGB / RAGB Antibody (aa1-30, FITC) - LS-C268593 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RRAGB / RAGB Antibody (aa1-30, Biotin) - LS-C268594 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RRAGB / RAGB Antibody (aa1-30, Azide-free, HRP) - LS-C268595 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RRAGB / RAGB Antibody (aa1-30) - LS-C324831 from LifeSpan Bioscience

Prices	$435.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RRAGB / RAGB Antibody (aa1-30, PE) - LS-C268596 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RRAGB / RAGB Antibody (C-Terminus) - LS-C186793 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of Human PANC1. RRAGB antibody dilution 1.0 ug/ml.

Anti-RRAGB / RAGB Antibody (aa1-30) - LS-C158898 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

RRAGB Antibody western blot of 293 cell line lysates (35 ug/lane). The RRAGB antibody detected the RRAGB protein (arrow).

RRAGB Antibody - H00010325-D01 from Novus Biologicals

Prices	$399.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: RRAGB Antibody [H00010325-D01]

RRAGB Antibody - NBP1-85801 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: RRAGB Antibody [NBP1-85801] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10<br/>Lane 2: Negative control (vector only transfected HEK293T lysate)<br/>Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416897)

RRAGB Antibody - H00010325-D01P from Novus Biologicals

Prices	$399.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Western Blot: RRAGB Antibody [H00010325-D01P] - Analysis of RRAGB expression in mouse brain.

RRAGB Antibody - H00010325-B01P from Novus Biologicals

Prices	$359.00
Sizes	50 µg
Host	Mouse
Clonality	Polyclonal

Western Blot: RRAGB Antibody [H00010325-B01P] - Analysis of RRAGB expression in transfected 293T cell line by RRAGB polyclonal antibody. Lane 1: RRAGB transfected lysate(41.14 KDa). Lane 2: Non-transfected lysate.

RRAGB Antibody - H00010325-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

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