Gene Symbol | POLR1B |
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Entrez Gene | 84172 |
Alt Symbol | RPA135, RPA2, Rpo1-2 |
Species | Human |
Gene Type | protein-coding |
Description | polymerase (RNA) I polypeptide B, 128kDa |
Other Description | DNA-directed RNA polymerase I 135 kDa polypeptide|DNA-directed RNA polymerase I 135kDa polypeptide|DNA-directed RNA polymerase I subunit RPA2|RNA polymerase I subunit 2 |
Swissprots | B7Z823 B7Z6Y7 Q6ZRR2 Q2TAM4 F8W898 F5GZX4 Q9H9D3 Q585T5 Q9H9Y6 |
Accessions | AAX81999 EAW73590 EAW73591 EAW73592 EAW73593 Q9H9Y6 AA484631 AK001678 BAA91831 AK022533 BAB14082 AK022890 BAB14296 AK025574 BAB15175 AK128044 BAC87247 AK172817 AK294027 BAH11652 AK301178 BAH13423 AK302814 BAH13809 BC004882 AAH04882 BC073857 AAH73857 BC096748 AAH96748 BC110833 AAI10834 BC150139 BX647360 BX647683 NM_001137604 NP_001131076 NM_001282772 NP_001269701 NM_001282774 NP_001269703 NM_001282776 NP_001269705 NM_001282777 NP_001269706 NM_001282779 NP_001269708 NM_019014 NP_061887 |
Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). {ECO:0000250}. |
Subcellular Location | Nucleus, nucleolus {ECO:0000269|PubMed:11250903}. |
Top Pathways | Purine metabolism, RNA polymerase, Pyrimidine metabolism |
RPA135 CRISPR/Cas9 KO Plasmid (h) - sc-402818 from Santa Cruz Biotechnology
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RPA135 HDR Plasmid (h) - sc-402818-HDR from Santa Cruz Biotechnology
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RPA135 Double Nickase Plasmid (h) - sc-402818-NIC from Santa Cruz Biotechnology
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RPA135 Double Nickase Plasmid (h2) - sc-402818-NIC-2 from Santa Cruz Biotechnology
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RPA135 CRISPR Activation Plasmid (h) - sc-402818-ACT from Santa Cruz Biotechnology
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RPA135 CRISPR Activation Plasmid (h2) - sc-402818-ACT-2 from Santa Cruz Biotechnology
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RPA135 Lentiviral Activation Particles (h) - sc-402818-LAC from Santa Cruz Biotechnology
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RPA135 Lentiviral Activation Particles (h2) - sc-402818-LAC-2 from Santa Cruz Biotechnology
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RPA135 siRNA (h) - sc-36436 from Santa Cruz Biotechnology
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RPA135 shRNA Plasmid (h) - sc-36436-SH from Santa Cruz Biotechnology
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RPA135 shRNA (h) Lentiviral Particles - sc-36436-V from Santa Cruz Biotechnology
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RPA2 RNAi - H00006118-R01 from Novus Biologicals
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POLR1B RNAi - H00084172-R01 from Novus Biologicals
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MISSION® esiRNA - EHU082821 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17107 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17107 from Sigma-Aldrich
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POLR1B siRNA (Human) - CRJ3335 from Cohesion Biosciences
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RPA135 CRISPR/Cas9 KO Plasmid (h2) - sc-402818-KO-2 from Santa Cruz Biotechnology
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RPA135 HDR Plasmid (h2) - sc-402818-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human POLR1B (NM_019014.4) - SHH379332 from Creative biogene
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POLR1B shRNA Plasmid (Human) - CHJ3335 from Cohesion Biosciences
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POLR1B miRNA 3'UTR clone - MiUTR3H-04587 from Creative biogene
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POLR1B miRNA 3'UTR clone - MiUTR3H-04588 from Creative biogene
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